Attenuation of spatial memory in 5xFAD mice by targeting cholinesterases, oxidative stress and inflammatory signaling using 2-(hydroxyl-(2-nitrophenyl)methyl)cyclopentanone

Ullah, Rahim; Ali, Gowhar; Subhan, Fazal; Khan, Ajmal; Halim, Sobia Ahsan; Naveed, Muhammad; Kalsoom, Saima; Al‐Harrasi, Ahmed

doi:10.1016/j.intimp.2021.108083

Cited by 9 publications

(3 citation statements)

References 88 publications

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“…We also examined the potential benefits of oral STX on hippocampal-dependent spatial memory. Several groups have previously reported behavioral deficits in aged 5XFAD mice using Morris Water Maze (MWM) tests ( Gu et al, 2018 ; Kang et al, 2018 ; Ullah et al, 2021 ), although these effects tend to be more subtle than in other behavioral assays. In female mice that were tested at 7–8 months of age, we did not detect any genotype differences between vehicle-treated 5XFAD and wild type animals in either the visible or hidden platform assays ( Fig.…”

Section: Resultsmentioning

confidence: 99%

The novel estrogen receptor modulator STX attenuates Amyloid-β neurotoxicity in the 5XFAD mouse model of Alzheimer's disease

Quinn¹,

Harris²,

Hack³

et al. 2022

Neurobiology of Disease

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Section: Resultsmentioning

confidence: 99%

The novel estrogen receptor modulator STX attenuates Amyloid-β neurotoxicity in the 5XFAD mouse model of Alzheimer's disease

Quinn¹,

Harris²,

Hack³

et al. 2022

Neurobiology of Disease

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“…Acetylthiocholine iodide (ATchI) and butyrylthiocholine iodide (BTchI) were used as substrates, and degradation of these substrates led to the formation of 5-thio-2-nitrobenzoate, which reacted with 5, 5'-dithiobis (2-nitro-benzoate (DTNB) forming a yellow color complex, which was assayed at 412 nm wavelength through a microplate reader. 30 The rate of absorbance (V = ΔAB/Δt) showed the enzyme activity (%) and inhibition by test/control samples and was calculated as follows, Enzyme inhibition ð%Þ¼ 100 enzyme activity % ð Þ…”

Section: In Vitro Studies In Vitro Cholinesterase Inhibition Assaymentioning

confidence: 99%

“…The Acacia stenophylla bark contains cyclic ketone derivatives, it has been reported that these derivatives reduce cholinesterases activities, Aβ plaques formation, neuroinflammation, and oxidative stress in the mice model of Alzheimer's disease. 30,72 The possible mechanism of action of Acacia stenophylla bark extracts to ameliorate amnesia is their anti-cholinesterase and anti-oxidant activities and the anti-inflammatory, anti-oxidant, and anti-cholinesterase properties of their active constituents derivatives. The schematic representation of ASEE and ASB extracts is shown in Figure 9.…”

mentioning

confidence: 99%

The Neuroprotective Propensity of Organic Extracts of Acacia stenophylla Bark and Their Effectiveness Against Scopolamine-/Diazepam-Induced Amnesia in Mice

Shah¹,

Iqbal²,

Alshehri³

et al. 2022

JIR

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Background: Alzheimer's disease (AD) is a neurodegenerative disorder that is more prevalent in the elderly. There is extensive literature on using Acacia species against central nervous system disorders, although Acacia stenophylla has not been investigated for any neuroprotective potential. The purpose of the study was to elucidate the ameliorative effect of ethyl acetate (ASEE) and butanol (ASB) extracts from the bark of A. stenophylla on memory deficits and cognitive dysfunction in scopolamine-or diazepam-induced amnesia in mice. Methods: The phytochemical constituents of the extracts of A. stenophylla were determined by GC-MS and the in vitro anticholinesterase plus antioxidant activities were also evaluated. Anti-amnesic effects were determined employing the open field test, elevated plus maze (EPM), Morris water maze (MWM), and Y-maze paradigms. Results: The in vitro cholinesterase assays disclosed a concentration-dependent inhibition of both AChE and BuChE with IC 50 values of 28.48 and 44.86 µg/mL for the ASEE extract and 32.04 and 50.26 µg/mL for the ASB extract against AChE and BuChE respectively. DPPH and H 2 O 2 antioxidant assays revealed respective IC 50 values for the ASEE extract of 28.04 and 59.84 µg/mL, plus 32.77 and 64.65 µg/mL for ASB extract. The findings revealed that both extracts possessed substantial antioxidant properties. Furthermore, these fractions restored scopolamine-and diazepam-induced memory deficits in a dose-dependent manner, as observed by a significant decrease in the transfer latency in EPM, reduction in escape latency, added time spent in the target quadrant in the MWM, and elevated spontaneous alternation behavior (SAB) in the Y-maze test. Conclusion:The ameliorative effect of A. stenophylla on scopolamine-and diazepam-induced amnesia can be attributed to antioxidant and anticholinesterase activity. Consequently, the use of A. stenophylla might be exploited in the alleviation of oxidative stress and the management of AD.

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Cassia artemisiodes attenuates nociceptive and diabetes-induced neuropathic pain modalities apropos antioxidant and anti-inflammatory mechanisms

Ullah

Badshah

Ali

et al. 2022

Biomedicine & Pharmacotherapy

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Attenuation of spatial memory in 5xFAD mice by targeting cholinesterases, oxidative stress and inflammatory signaling using 2-(hydroxyl-(2-nitrophenyl)methyl)cyclopentanone

Cited by 9 publications

References 88 publications

The novel estrogen receptor modulator STX attenuates Amyloid-β neurotoxicity in the 5XFAD mouse model of Alzheimer's disease

The novel estrogen receptor modulator STX attenuates Amyloid-β neurotoxicity in the 5XFAD mouse model of Alzheimer's disease

The Neuroprotective Propensity of Organic Extracts of Acacia stenophylla Bark and Their Effectiveness Against Scopolamine-/Diazepam-Induced Amnesia in Mice

Cassia artemisiodes attenuates nociceptive and diabetes-induced neuropathic pain modalities apropos antioxidant and anti-inflammatory mechanisms

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