Attenuating effects of omega-3 fatty acids (Omegaven) on irradiation-induced intestinal injury in mice

Sun, Mingli; Pang, Lei; XuePeng, Ju; Sun, Hai-Gang; Yu, Jie-Ping; Zhao, Haishan; Yao, Weifan

doi:10.1016/j.fct.2013.11.051

Cited by 7 publications

(8 citation statements)

References 25 publications

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“…More recently, an omega-3 polyunsaturated fatty acid-derived metabolite, Resolvin E1 was documented to inhibit inflammatory responses and apoptotic damage in sepsis-induced cardiomyopathy, which is in accordance with our discovery . Further in line with our findings, Sun et al demonstrated that omega-3 fatty acids also possess the ability to ameliorate irradiation-induced gastrointestinal injury in BALB/c mouse models by diminishing TNF-α, IL-6 and MDA levels, as well as increasing SOD activity (Sun et al 2014). Altogether, these findings and data make it plausible to suggest that omega-3 FAs exhibit a protective effect against sepsis.…”

Section: Discussionsupporting

confidence: 90%

Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injury

Chen

Xie

Liu

et al. 2022

Mol Med

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Background Sepsis is a troublesome syndrome that can cause intestinal injury and even high mortality rates. Omega-3 fatty acids (FAs) are known to protect against intestinal damage. Accordingly, the current study set out to explore if omega-3 FAs could affect sepsis-induced intestinal injury with the involvement of the microRNA (miR)-1-3p/Notch3-Smad axis. Methods First, cecal ligation and perforation (CLP) was performed to establish septic mouse models in C57BL/6J mice, and mouse intestinal epithelial MODE-K cells were induced by lipopolysaccharide (LPS) to establish sepsis cell models. The CLP-induced septic mice or LPS-exposed cells were subjected to treatment with Omega-3 FAs and activin (Smad signaling activator), miR-1-3p inhibitor and over-expressed/short hairpin RNA (oe-/sh)-Notch3 to explore their roles in inflammation, intestinal oxidative stress and cell apoptosis. A dual-luciferase reporter gene assay was further performed to verify the regulatory relationship between miR-1-3p and Notch3. Results Omega-3 FAs inhibited CLP-induced intestinal injury and ameliorated LPS-induced intestinal epithelial cell injury by down-regulating miR-1-3p, as evidenced by decreased levels of tumor necrosis factor-α, interleukin-1β (IL-1β) and IL-6, in addition to diminished levels of reactive oxygen species, malondialdehyde levels and superoxide dismutase activity. Furthermore, miR-1-3p could down-regulate Notch3, which inactivated the Smad pathway. Conclusion Collectively, our findings indicated that omega-3 FAs elevate the expression of Notch3 by down-regulating miR-1-3p, and then blocking the Smad pathway to alleviate intestinal epithelial inflammation and oxidative stress injury caused by sepsis.

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Section: Discussionsupporting

confidence: 90%

Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injury

Chen

Xie

Liu

et al. 2022

Mol Med

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“…However, mice injected with 5-FU and fed a diet with n-3 showed decreased mucosal inflammation with maintenance of the villus and crypt length, making the MUC + n-3 mice similar to the CTL mice. It was previously described that n-3 FA consumption significantly attenuates intestinal injury [ 22 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Increased apoptosis occurs due to DNA condensation and the activation of caspases with decreased proliferation of villus cells [ 38 , 39 ]. By contrast, increased EPA or DHA concentrations in the membrane of cells reduce caspase 9 and 3 activation and decrease cytochrome c release, which regulates cellular oxidative stress and controls endothelial cell dysfunction [ 36 , 40 ]. Using a TUNEL assay, we observed a decreased rate of apoptotic cells in the ileum mucosa of n-3 treated mice that underwent mucositis compared to mice that did not receive n-3 FA.…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with omega-3 fatty acid attenuates 5-fluorouracil induced mucositis in mice

et al. 2015

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BackgroundStudies showed the positive effects of omega-3 fatty acid (n-3 FA) for the treatment of inflammatory bowel disease as it alleviated the symptoms and promoted better mucosal integrity. The objective of this study was to determine whether a diet with the addition of n-3 FA helps control the inflammation observed in 5-fluorouracil (5-FU) induced mucositis.MethodsBALB/c mice were randomly divided into four groups as follows: 1: control (CTL), fed a standard chow diet; 2: CTL + n-3 FA – n-3 FA, fed a diet with n-3; 3: mucositis (MUC), fed a standard chow diet and subjected to mucositis; and 4: MUC+ n-3 FA, fed a diet with n-3 FA and subjected to mucositis. On the 8th day, the animals of the MUC and MUC + n-3 FA groups received an intraperitoneal injection of 300 mg/kg 5-FU for mucositis induction. After 24 h or 72 h, all mice were euthanized and evaluated for intestinal permeability, bacterial translocation, intestinal histology and apoptosis.ResultsMice that received the diet with n-3 FA and a 5-FU injection showed less weight loss compared to the animals of the MUC group (p < 0.005). Decreased intestinal permeability and bacterial translocation were also observed in animals fed n-3 FA, and these mice underwent mucositis compared to the MUC group (p < 0.005). These data were associated with mucosal integrity and a reduced number of apoptotic cells in the ileum mucosa compared to the mice that received the control diet and 5-FU injection.ConclusionTogether, these results show that omega-3 fatty acid decreases the mucosal damage caused by 5-FU-induced mucositis.

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“…A few animal studies have explored the protective effects on PUFAs concerning irradiation (Tables 1 and 2). Injury of the gastrointestinal (GI) tract caused by irradiation reduces villi to crypt height ratio and increases pro-inflammatory cytokines in the GI tract (Sun et al 2014). PUFAs are known to protect against radiation-induced GI disorders such as ulcerative colitis or short bowel syndrome by reducing inflammation (Sun et al 2014).…”

Section: Test Subjectmentioning

confidence: 99%

“…Injury of the gastrointestinal (GI) tract caused by irradiation reduces villi to crypt height ratio and increases pro-inflammatory cytokines in the GI tract (Sun et al 2014). PUFAs are known to protect against radiation-induced GI disorders such as ulcerative colitis or short bowel syndrome by reducing inflammation (Sun et al 2014). In the study by Sun et al, mice were supplemented with or without PUFAs (Omegaven) and then subjected to 4 Gy/min rate of irradiation.…”

Section: Test Subjectmentioning

confidence: 99%

Low dose radiation, inflammation, cancer and chemoprevention

Rashid

Ramalingam

Moustaid‐Moussa

et al. 2018

International Journal of Radiation Biology

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Overall, LDR exerts mainly damaging effects through diverse cell and molecular mechanisms, with a few beneficial effects reported. In some cancers, surrounding adipose tissue of the breast may contribute to obesity-related cancer. Further, preclinical data suggest that anti-inflammatory dietary compounds such as PUFA and other dietary interventions may protect against radiation effects on cancer cells and cancer progression.

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Attenuating effects of omega-3 fatty acids (Omegaven) on irradiation-induced intestinal injury in mice

Cited by 7 publications

References 25 publications

Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injury

Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injury

Dietary supplementation with omega-3 fatty acid attenuates 5-fluorouracil induced mucositis in mice

Low dose radiation, inflammation, cancer and chemoprevention

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