Attenuated infrared neuron stimulation response in cochlea of deaf animals may associate with the degeneration of spiral ganglion neurons

Xie, Bingbin; Dai, Chenyang; Li, Huawei

doi:10.1364/boe.6.001990

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…IR heat pulse stimuli evoke both excitatory and inhibitory responses in the semicircular canals irrespective of orientation of the optical stimulus relative to hair bundle orientation (Rajguru et al 2011), which is inconsistent with activation by thermal expansion of the endolymph or optoacoustic activation of MET currents. Although auditory hair cells are sensitive to the photoacoustic component of pulsed optical stimuli (Schultz et al 2012;Tan et al 2015;Thompson et al 2015;Xie et al 2015), the present work is focused on photothermal mechanisms, which dominate activation of the crista ampullaris in the IR stimulus range used in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…Summary and conclusion. It has been shown previously that optical heat pulse stimulation of the cochlea can lead to transduction through photoacoustic mechanical activation of hair cell MET currents (Schultz et al 2012;Tan et al 2015;Thompson et al 2015;Xie et al 2015). Unfortunately, hair cell photoacoustic sensitivity is usually completely absent in cases of severe auditory and vestibular loss, rendering the photoacoustic component of the response to optical stimulation unavailable as a means to restore cochlear or vestibular sensation.…”

Section: Resultsmentioning

confidence: 99%

“…Auditory and vestibular hair cells are very sensitive to pulsed IR stimulation. In the organ of Corti, pulsed IR stimulation evokes robust neural responses in the healthy hearing cochlea that have origins primarily in stimulation of sensory hair cells (Izzo et al 2006;Schultz et al 2012;Tan et al 2015;Thompson et al 2015;Xie et al 2015). Part of the response in cochlear hair cells is mechanical and arises from optoacoustic gating of MET channels (Fridberger and Ren 2006;Schultz et al 2012), whereas the remainder is thermal.…”

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confidence: 99%

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Heat pulse excitability of vestibular hair cells and afferent neurons

Rabbitt

Brichta²,

Tabatabaee

et al. 2016

Journal of Neurophysiology

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In the present study we combined electrophysiology with optical heat pulse stimuli to examine thermodynamics of membrane electrical excitability in mammalian vestibular hair cells and afferent neurons. We recorded whole cell currents in mammalian type II vestibular hair cells using an excised preparation (mouse) and action potentials (APs) in afferent neurons in vivo (chinchilla) in response to optical heat pulses applied to the crista (ΔT ≈ 0.25°C per pulse). Afferent spike trains evoked by heat pulse stimuli were diverse and included asynchronous inhibition, asynchronous excitation, and/or phase-locked APs synchronized to each infrared heat pulse. Thermal responses of membrane currents responsible for APs in ganglion neurons were strictly excitatory, with Q10 ≈ 2. In contrast, hair cells responded with a mix of excitatory and inhibitory currents. Excitatory hair cell membrane currents included a thermoelectric capacitive current proportional to the rate of temperature rise (dT/dt) and an inward conduction current driven by ΔT An iberiotoxin-sensitive inhibitory conduction current was also evoked by ΔT, rising in <3 ms and decaying with a time constant of ∼24 ms. The inhibitory component dominated whole cell currents in 50% of hair cells at -68 mV and in 67% of hair cells at -60 mV. Responses were quantified and described on the basis of first principles of thermodynamics. Results identify key molecular targets underlying heat pulse excitability in vestibular sensory organs and provide quantitative methods for rational application of optical heat pulses to examine protein biophysics and manipulate cellular excitability.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Heat pulse excitability of vestibular hair cells and afferent neurons

Rabbitt

Brichta²,

Tabatabaee

et al. 2016

Journal of Neurophysiology

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“…INS was previously used to evoke auditory responses oABRs in gerbils, guinea pigs 2 , 59 , 60 , mice 14 , and cats 61 . However, no successful stimulation of congenitally deaf animals has thus far been reported.…”

Section: Discussionmentioning

confidence: 99%

Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation

Tan

Jahan

et al. 2018

Sci Rep

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To determine whether responses during infrared neural stimulation (INS) result from the direct interaction with spiral ganglion neurons (SGNs), we tested three genetically modified deaf mouse models: Atoh1-cre; Atoh1 f/f (Atoh1 conditional knockout, CKO), Atoh1-cre; Atoh1 f/kiNeurog1 (Neurog1 knockin, KI), and the Vglut3 knockout (Vglut3 −/−) mice. All animals were exposed to tone bursts and clicks up to 107 dB (re 20 µPa) and to INS, delivered with a 200 µm optical fiber. The wavelength (λ) was 1860 nm, the radiant energy (Q) 0-800 µJ/pulse, and the pulse width (PW) 100–500 µs. No auditory responses to acoustic stimuli could be evoked in any of these animals. INS could not evoke auditory brainstem responses in Atoh1 CKO mice but could in Neurog1 KI and Vglut3 −/− mice. X-ray micro-computed tomography of the cochleae showed that responses correlated with the presence of SGNs and hair cells. Results in Neurog1 KI mice do not support a mechanical stimulation through the vibration of the basilar membrane, but cannot rule out the direct activation of the inner hair cells. Results in Vglut3 −/− mice, which have no synaptic transmission between inner hair cells and SGNs, suggested that hair cells are not required.

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“…Using these lower-cost diode systems (∼$20k), multiple groups published results demonstrating the efficacy of 1875 nm and 1450 nm light for eliciting neural activity. 9 , 22 , 26 , 27 , 30 – 32 , 36 – 51 Custom diode systems can be made for a few thousand dollars but require some proficiency in laser hardware and electronics. Other groups opted to use fiber lasers with tunable thulium fiber lasers centered around 2000 nm being the most common, but this wavelength may be too strongly absorbed by water in the tissue.…”

Section: Introductionmentioning

confidence: 99%