Attempts to Modulate the Immune Response to a Hapten-Carrier Complex with Various Hapten-Containing Compounds

Abstract

The immune response to a hapten-carrier conjugate appears to be a complex phenomenon where reactions of the T-cell population are not restricted to the carrier and where the reactions of the B-cell population are not limited to the hapten determinant of the antigen molecule. To get a better understanding of the different cell interactions during the immune response to a hapten-carrier complex, the effects of immunogenic or tolerogenic injections of various hapten-containing compounds on the responses induced by immunization with the same hapten coupled to protein carriers were studied. The results indicate that T cells involved in delayed hypersensitivity and T cells involved in contact dermatitis could belong to distinct subclasses and confirm that hapten and carrier moieties of the antigen molecule could compete, probably at the macrophage level, for both delayed hypersensitivity to the carrier and antibody synthesis to the hapten.

Carrier and Hapten Functions in Immune Deviation

“…Similar results have been previously obtained by the intravenous injection of the hapten coupled to an unrelated protein carrier [13] or by the intravenous injection of dinitrobenzene sulfonate (DNBSO3) [12] just before immunization with hapten carrier protein in FCA. Variations in op posite directions of both cellular reactions to the pro tein carrier and humoral reactions to the hapten was previously described to reflect antigenic competition between hapten and carrier determinants of the anti gen molecule […”

Polyclonal Activation of Guinea Pig Spleen Lymphocytes

“…In the guinea pig, helper T cells have been already functionally dis sociated from T cells involved in delayed hypersensi tivity [Neveu and Borduas, 1974;Silver and Benacerraf, 1974], Similarly, T cells involved in delayed hy persensitivity and T cells involved in contact dermati tis could belong to distinct T cell subclasses [Neveu, 1979].…”

Delayed-Type Hypersensitivity as Revealed on the Footpads of Mice to Azobenzenearsonate-Acetyl Bovine Serum Albumin

“…Footpad reaction [4], ear swelling reaction [5], flank skin reaction [6], and percutaneous skin re action [7] have well been established for the induction of DTH in mice [8,9], rats [10,11] or guinea pigs [12,13]. There are varying test antigens: bovine serum albumin [14], ovalbumin [15], lysozyme [16], meth ylated human serum albumin [17], sheep red blood cells [18], and so forth [19]. Azobenzenearsonate-protein conjugate has been used in the immunochemical study of anti genic specificity [20] and considered to be a suitable antigen to induce delayed flank skin reaction in guinea pigs [21] or rats [22], Therefore, it appeared constructive to ex amine the DTH footpad reaction in mice to azobenzenearsonate-acetyl bovine serum al bumin (ABA-AcBSA) in detail.…”