Attachment of sponge cells to collagen substrata: Effect of a collagen assembly factor

Abstract

Collagen, isolated from the sponge Geodia cydonium in the absence of denaturing agents, had the typical amino acid composition and was associated with the carbohydrates galactose and glucose. The resulting individual fibrils with a diameter of 23 nm, displayed a 19.5 nm periodicity with one intraperiod band. A collagen assembly factor (CAF) was identified in and partially purified from the extracellular space. The CAF reacted with antibodies against intact Geodia cells but not with antibodies against Geodia lectin and Geodia aggregation factor. In the presence of the CAF, the collagen fibrils reconstituted collagen bundles in an ordered sequence of events, which were followed by electron-microscopical and biochemical methods. Bundle formation was not dependent on the presence of the homologous lectin, glycoconjugates or aggregation factor. Homologous cells (Geodia archaeocytes) were determined to attach only to those Geodia collagen substrates that contained CAF. The attachment of these cells did not require fibronectin or Geodia lectin. Homologous glycoconjugates or NaOH-treated collagen inhibited cell attachment. Collagen from the sponge Chondrosia reniformis, even in the presence of Geodia CAF, was no appropriate substrate for Geodia cell attachment. Whether collagen is a component of cell-matrix interactions in sponge systems also in vivo is discussed.

Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (Chondrilla caribensis and Aplysina fulva): Physicochemical Properties and In Vitro Biological Analysis

“…These findings are in agreement with [19] who also observed that trypsin digestion at an optimum alkaline pH range resulted in a higher amount of Col. Barros et al (2015) also successfully extracted Col/gelatin using water acidified with carbon dioxide. Interestingly, previous works have stated that Col from marine sponge was insoluble in dilute acid pH mediums [21,33]. However, this behavior was not found in the present study with the use of slightly acidic pH (P4), possibly due to the presentation of the samples (fine powder).…”

Origin of Metazoa: Sponges as Living Fossils

“…However, a new class of collagens has been identified in fungi which are thought to have formed by convergence [47]. Primitive fibrillar collagens have been visualized in a series of species in sponges, including Chondrosia reniformis [48] and G. cydonium [49]. The 20-to 25-nm-diameter fibrils from G. cydonium display a 19.5-nm periodicity with one intraperiod band.…”

“…A collagen assembly factor has been identified from the ECM material which causes a reconstitution of collagen bundles in an ordered sequential event (Fig. 1c) [49]. Exposito and Garrone [50] were the first to identify a collagen of this type on cDNA level.…”