Attachment of human bone cells to tissue culture polystyrene and to unmodified polystyrene: the effect of surface chemistry upon initial cell attachment

Steele, John G.; McFarland, Clive D.; Dalton, B. A.; Johnson, Graham; Evans, Margaret D. M.; Howlett, C. R.; Underwood, P.A.

doi:10.1163/156856293x00339

Cited by 130 publications

(68 citation statements)

References 42 publications

(50 reference statements)

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“…The topographical features of a scaffold have a significant effect in tissue engineering and it has been demonstrated that the surface roughness of scaffolds affects cell behaviour, including cell attachment, proliferation and differentiation (Chen et al, 2007;Naji and Harmand, 1990;Meyer et al, 1993;Steele et al, 1993;Chu et al, 1999;Xu et al, 2004b). Moreover, the topographical features are described to have a positive effect on axonal orientation, where the physical guidance of axons is a vital component of nerve repair.…”

Section: Topographical Modification Of Conductive Scaffoldsmentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

603

389

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Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

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Section: Topographical Modification Of Conductive Scaffoldsmentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

603

389

View full text Add to dashboard Cite

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“…Bone-derived cells were prepared from explant cultures as described previously, 22 using bone collected during surgery from patients under 12 years of age who were not suffering from conditions known to affect bone metabolism. Explant cultures were established in 25-cm 2 flasks (Nunc, Roskilde, Denmark) in culture medium 199 supplemented with 10% (v/v) fetal bovine serum (FBS; PA Biologicals, Sydney, NSW, Australia), 2 mM glutamine, 30 g/mL penicillin, 100 g/mL streptomycin, and 50 g/mL ascorbate.…”

Section: Cell Culturementioning

confidence: 99%

“…Since in media containing FBS, HBDC attachment to glass is due primarily to Vn adsorbed from the serum, 22 this substrate was used as a positive control. As shown in Figure 6, HBDC attached and spread on glass within 2 h of being seeded in medium containing 10% normal FBS.…”

Section: Receptor Expression In Attached Cellsmentioning

confidence: 99%

Protein adsorption and cell attachment to patterned surfaces

McFarland

Thomas

DeFilippis

et al. 2000

J. Biomed. Mater. Res.

Self Cite

142

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To better understand the events involved in the generation of defined tissue architectures on biomaterials, we have examined the mechanism of attachment of human bone-derived cells (HBDC) to surfaces with patterned surface chemistry in vitro. Photolithography was used to generate alternating domains of N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) and dimethyldichlorosilane (DMS). At 90 min after seeding, HBDC were localized preferentially to the EDS regions of the pattern. Using sera specifically depleted of adhesive glycoproteins, this spatial organization was found to be mediated by adsorption of vitronectin (Vn) from serum onto the EDS domains. In contrast, fibronectin (Fn) was unable to adsorb in the face of competition from other serum components. These results were confirmed by immunostaining, which also revealed that both Vn and Fn were able to adsorb to EDS and DMS regions when coated from pure solution, i.e., in the absence of competition. In this situation, each protein was able to mediate cell adhesion across a range of surface densities. Cell spreading was constrained on the EDS domains, as indicated by cell morphology and the lack of integrin receptor clustering and focal adhesion formation. This spatial constraint may have implications for the subsequent expression of differentiated function.

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“…15 However, it is now understood that the surface properties of biomaterials play a critical role in the establishment of cell-biomaterial interfaces. In vitro cytocompatibility studies are increasingly concerned with the influence of surface topography, [16][17][18] surface charge, [19][20][21] and consecutive adsorption of proteins 7,8,[22][23][24][25][26] on cell attachment and proliferation. Many studies demonstrate that the surface topography is an important parameter to consider.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses

Anselme

Bigerelle

Noël

et al. 2000

J. Biomed. Mater. Res.

441

197

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We quantitatively evaluated the adhesion of human osteoblasts on orthopedic metallic substrates (Ti6Al4V alloy) with various surface roughnesses at several times after inoculation and studied its correlation with qualitative changes in the expression of adhesion proteins and with parameters extensively describing the surface topographies. Cells were orientated in a parallel order on polished surfaces. This orientation was not affected by residual grooves after polishing. On sandblasted surfaces the cells never attained confluence and had a stellate shape, and the cell layer had no particular organization. Extracellular matrix (fibronectin, type I collagen, osteopontin) and cytoskeletal protein (actin, vinculin) orientation reflected the cell layer organization. In our experiment human osteoblasts expressed alpha3beta1 integrin but not alpha2beta1 integrin. In addition to currently analyzed roughness magnitude parameters, we calculated roughness organization parameters (fractal dimension parameters) of the substrates. We observed lower adhesion and proliferation on less organized surfaces (i.e., sandblasted ones). The significant statistical correlation observed between fractal dimension parameters (describing surface roughness organization) and cell parameters adds a new concept to the studies of substratum roughness influence on cell behavior. An attempt at modelization of the cell-surface interaction was made that includes the influence of fractal dimensions parameters.

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Attachment of human bone cells to tissue culture polystyrene and to unmodified polystyrene: the effect of surface chemistry upon initial cell attachment

Cited by 130 publications

References 42 publications

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Protein adsorption and cell attachment to patterned surfaces

Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses

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