Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses

Abstract

We quantitatively evaluated the adhesion of human osteoblasts on orthopedic metallic substrates (Ti6Al4V alloy) with various surface roughnesses at several times after inoculation and studied its correlation with qualitative changes in the expression of adhesion proteins and with parameters extensively describing the surface topographies. Cells were orientated in a parallel order on polished surfaces. This orientation was not affected by residual grooves after polishing. On sandblasted surfaces the cells never attained confluence and had a stellate shape, and the cell layer had no particular organization. Extracellular matrix (fibronectin, type I collagen, osteopontin) and cytoskeletal protein (actin, vinculin) orientation reflected the cell layer organization. In our experiment human osteoblasts expressed alpha3beta1 integrin but not alpha2beta1 integrin. In addition to currently analyzed roughness magnitude parameters, we calculated roughness organization parameters (fractal dimension parameters) of the substrates. We observed lower adhesion and proliferation on less organized surfaces (i.e., sandblasted ones). The significant statistical correlation observed between fractal dimension parameters (describing surface roughness organization) and cell parameters adds a new concept to the studies of substratum roughness influence on cell behavior. An attempt at modelization of the cell-surface interaction was made that includes the influence of fractal dimensions parameters.

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

“…The roughness of a biomaterial-based graft is an important factor due to its e ect on various cellular behaviors such as protein adsorption, cell attachment, and cell proliferation [12,13]. Similar to the results reported by Milleret et al [14], surface roughness increased with increasing constituent ber diameter (Figure 2).…”

Hydrofluoric acid etching of dental zirconia. Part 1: etching mechanism and surface characterization

“…This constitutes an interesting result given the high sensitivity of osteoblasts to roughness at different scales [7,9,[38][39][40][41] and the strong influence of fractal dimension on osteoblastic adhesion and differentiation [10]. Regarding the roughness parameters, although the lack of standardization in the measurements makes comparison difficult with other studies, the values of S a , S dr , S ds and S ci which were obtained here fall within the range of the reported values for commercial dental implants with proved high success rate [8,[42][43][44].…”

“…On the other hand, a complementary approach to the classical roughness parameters calculation is to perform a fractal analysis. It has been demonstrated that osteoblastic cells proliferation and adhesion is strongly correlated to fractal parameters [10]. There is therefore a strong interest in developing processes which allow controlling the roughness and the fractal dimension of a surface.…”

Microtopography of metal surfaces influence fibroblast growth by modifying cell shape, cytoskeleton, and adhesion

“…Their definition of surface morphology is based on the correlation of numerous measurements, such as roughness organization, rather that simple height averages; these measures were observed to elicit significant osteoblast reactivity. [29][30][31] Our definition is based on a culmination of visual and numerical characterizations, 9 coupled with correlation of these features to specific cellular features. In the instance of NS, the surface morphology is micro-spiked, produced by the protruding b-phase particles, and as our evidence indicates, it is the differing and critical aspect of this specific topography that affects the cells.…”