Attachment of Con A or extracellular matrix initiates rapid sprouting by cultured leech neurons.

Chiquet, Matthias; Acklin, Susan E.

doi:10.1073/pnas.83.16.6188

Cited by 67 publications

(97 citation statements)

References 26 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The peripheral motile regions of leech neurites are rich in micro¢laments Retzius and anterior pagoda (AP) neurons were cultured on concanavalin A (ConA), on which they extend broad, veil-like neurites that allow for detailed analysis of the cytoskeleton (Chiquet & Acklin 1986;Neely 1993;Neely & Gesemann 1994). In phase contrast microscopy, phase-dark areas were often situated in the periphery of these lamellipodia and at the base of ¢lopodia (¢gure 1a and ¢gure 2a).…”

Section: R E Su Lt S (A)mentioning

confidence: 99%

Motile areas of leech neurites are rich in microfilaments and two actin–binding proteins: gelsolin and profilin

Neely

MaCaluso

1997

Proc. R. Soc. Lond. B

View full text Add to dashboard Cite

Cell motility is produced by changes in the dynamics and organization of actin ¢laments. The aim of the experiments described here was to test whether growing neurites contain two actin-binding proteins, gelsolin and pro¢lin, that regulate polymerization of actin and a¡ect non-neuronal cell motility. The distribution of gelsolin, pro¢lin and the micro¢laments was compared by immunocytochemistry of leech neurons growing in culture. We observed that micro¢laments are enriched in the peripheral motile areas of the neurites. Both gelsolin and pro¢lin are also concentrated in these regions. Gelsolin is abundant in ¢lopodia and is associated with single identi¢able micro¢lament bundles in lamellipodia. Pro¢lin is not prominent in ¢lopodia and shows a di¡use staining pattern in lamellipodia. The colocalization of gelsolin and pro¢lin in motile, micro¢lament-rich areas supports the hypothesis that they synergistically regulate the actin dynamics that underlie neurite growth.

show abstract

Section: R E Su Lt S (A)mentioning

confidence: 99%

Motile areas of leech neurites are rich in microfilaments and two actin–binding proteins: gelsolin and profilin

Neely

MaCaluso

1997

Proc. R. Soc. Lond. B

View full text Add to dashboard Cite

show abstract

“…Briefly, segmental ganglia were removed from the CNS of anaesthetized animals (chlorobutanol 0.15%; Sigma, Milan, Italy). Neuronal cell bodies of the ventral lateral packet were collected and plated onto concanavalin A (Sigma)-coated COC substrates [47]. Cells were maintained in Leibovitz-15 medium supplemented with 0.6% glucose, 2% fetal calf serum and gentamicin 200 mM (Sigma), at 208C for 2 -4 days.…”

Section: Leech Neuronal Cell Culture and Immunostainingmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

View full text Add to dashboard Cite

Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.

show abstract

“…The number of trials used for averaging was lower than for the records made from cells on Con A. Similar experiments were also made on AP and P cells. In culture they too branch differently on Con A and on leech laminin extract upon which they have finer processes (3 …”

mentioning

confidence: 99%

“…One example is the Retzius cell (1), which has a neuromodulatory function in vivo, secretes serotonin, and forms chemical synapses on P mechanosensory cells in culture. On Con A as substrate, Retzius cells sprout neurites with broad flattened growth cones and stout processes that tend to fasciculate, whereas on leech laminin extract as substrate, outgrowing neurites have small growth cones, and their processes are long and extremely fine with multiple branches (3,4).…”

mentioning

confidence: 99%

“…The extent of neurite outgrowth, the neurite branching pattern, and the shape of the growth cones are markedly dependent on the growth substrate. Neurons that grow only slowly on polylysine sprout processes rapidly and extensively on the plant lectin Con A or on a more natural substrate, a laminin-like molecule extracted from the extracellular matrix (ECM) of the leech central nervous system (3,4), referred to here as "leech laminin extract." Once it starts to grow, each type of cell has a characteristic fingerprint that depends on its identity and on the substrate.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Influence of substrate on the distribution of calcium channels in identified leech neurons in culture.

Ross

Aréchigá

Nicholls

1988

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The Retzius neuron from the leech, growing in culture on the plant lectin concanavalin A as substrate, produces broad flat growth cones and thick bundles of processes. The same cell extends rme straight processes with numerous branches when grown on a lanminin-like substrate extracted from leech central nervous system extracellular matrix, referred to as "leech laminin extract." Cells growing on these two different substrates also show marked differences in the pattern of Ca2" entry following evoked impulses, as detected optically by local changes in absorbance of the Ca2 -sensitive dye arsenazo m. Ca2+ enters the soma and initial segment of Retzius cells grown on both substrates. However, detectable Ca2+ entry only occurs into the processes of cells growing on leech laminin but not of those growing on concanavalin A. Optical recordings of changes in membrane potential made with the voltage-sensitive dye RH 155 taken from cells growing on either substrate indicate that a depolarization initiated in the soma spreads to the most distant processes with little or no distortion in amplitude or time course. This implies that all voltage-sensitive Ca2+ channels in the cell membrane are equally activated by depolarizing stimuli. Therefore, the fact that impulses evoke Ca2+ entry into processes of Retzius cells grown on leech laminin extract but not of cells grown on concanavalin A shows that the nature of the growth substrate can affect the number and distribution of their functional Ca2+ channels.

show abstract

Attachment of Con A or extracellular matrix initiates rapid sprouting by cultured leech neurons.

Cited by 67 publications

References 26 publications

Motile areas of leech neurites are rich in microfilaments and two actin–binding proteins: gelsolin and profilin

Motile areas of leech neurites are rich in microfilaments and two actin–binding proteins: gelsolin and profilin

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Influence of substrate on the distribution of calcium channels in identified leech neurons in culture.

Contact Info

Product

Resources

About