Influence of substrate on the distribution of calcium channels in identified leech neurons in culture.

Ross, William N.; Aréchigá, Hugo; Nicholls, John G.

doi:10.1073/pnas.85.11.4075

Cited by 44 publications

(25 citation statements)

References 10 publications

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“…Different substrates alter both the ionic conductance properties and the overall morphology of leech Retzius neurones (Ross, Arechiga & Nicholls, 1988). Nerves grown on con A had broad, flat growth cones and (Jaffe & Poo, 1979;Patel & Poo, 1982;McCaig, 1986).…”

Section: Culturei) Nervis In An Applifi) Ei I Ctric F1111)mentioning

confidence: 99%

Electrical fields, nerve growth and nerve regeneration

McCaig

Rajnicek²

1991

Experimental Physiology

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The presence of voltage gradients within developing and damaged tissues led to the notion that the resultant electrical fields provide instructional cues to cells. Field effects on avian and amphibian neurones in vitro include increased differentiation, turning of neurites towards the cathode, increased rate of growth towards the cathode, resorption of anodefacing neurites, increased branching and increased filopodial activity. Electric fields enhance regeneration of damaged PNS and CNS neurones in animals as diverse as lampreys, frogs, rats and guinea‐pigs, but the mechanisms by which fields produce their effects are not understood. Further examination of the interaction of fields with intracellular elements, such as the cytoskeleton and second messenger systems, may offer some insight.

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Section: Culturei) Nervis In An Applifi) Ei I Ctric F1111)mentioning

confidence: 99%

Electrical fields, nerve growth and nerve regeneration

McCaig

Rajnicek²

1991

Experimental Physiology

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“…The use of Con A as a substrate significantly increased the proportion of neurones that extended neurites. Con A has disadvantages as a substrate, the most important of which is that it reduces the likelihood of formation of chemical synapses (Lin & Levitan, 1987;Ross, Arechiga & Nicholls, 1988). Of the conditions tested, by far the best outgrowth of neurites was seen when the cells were plated onto Falcon 3001 culture dishes coated with poly-L-lysine in the presence of CM.…”

Section: Culture Conditionsmentioning

confidence: 99%

Properties of an identified dopamine‐containing neurone in culture from the snail Helisoma

Harris¹,

Cottrell²

1995

Experimental Physiology

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SUMMARYThe giant neurone in the left pedal ganglion of the snail Helisoma trivoli'is is homologous with the giant dopaminergic neurone of Planorbis corneus, because the neurones have a very similar location and morphology, and react similarly with glyoxylic acid to produce an intense blue fluorescence, indicating the presence of dopamine. Each of these neurones is therefore referred to as a giant dopaminergic neurone, or GDN. Conditions for the extension of neurites and formation of chemical junctions in culture have been determined for the H. trivolvis GDN, and compared with other neurones from this species. The pattern of neurites that extended from the neurone was indistinguishable from that of another identified aminergic neurone, the large serotonergic neurone (LSN), but differed markedly from many other central neurones. However, the type of substrate also greatly affected the pattern of the neurites observed. Some of the electrical properties of the GDN in culture differed from those recorded in situ: peak spike amplitude was increased, spike half-width reduced and the firing pattern of the neurone was altered. However, the resting membrane potential was very similar. The GDN formed chemical and electrical junctions in culture. The chemical junctions formed were of the same type as those found in situ. They formed rapidly, within 18 h after plating, but were not stable and were lost within 48 h, to be replaced by a non-rectifying electrical junction. A chemical junction may form in either direction between the GDN and the LSN, but only rarely did such junctions allow transmission in both directions, as observed in situ. Experiments in which neurones were plated out at different times suggested that the direction of formation of the chemical junction was not dependent on the degree or state of neurite extension.

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“…For example, leech neurons in vitro show no selectivity for growth on two different substrates but the morphology of the neurites (Chiquet and Nicholls, 1987) and the localization of Ca*+ channels on the neurites (Ross et al, 1988) differ depending on the substrate. Similarly, neurites of sympathetic neurons in vitro express either axonal or dendritic properties depending on the substrate (Lein and Higgins, 1989).…”

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confidence: 99%

Neurite growth on different substrates: permissive versus instructive influences and the role of adhesive strength

et al. 1992

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Growing axons use environmental cues to guide them to their targets. One class of cues is thought to be adhesion molecules on cells and in the extracellular matrix that axons interact with as they grow to their targets. In choosing between two possible pathways, the relative adhesiveness of the two substrates could be an important factor in controlling neurite growth. We conducted experiments in vitro to study how naturally occurring adhesion molecules influence neurite growth. Neurite growth rates, the degree of neurite fasciculation, the choices neurites make between two substrates, and the relative adhesiveness of different substrates were examined. We found that the relative adhesiveness of a substrate was a poor predictor of either axon growth rate or the degree of fasciculation. Furthermore, neurites showed little selectivity between three different naturally occurring substrates, L1, N-cadherin, and laminin. These results suggest that some adhesion molecules may serve as permissive substrates in that they can define axonal pathways but they do not provide information about which path to take at a choice point or about which direction to go along the path. Finally, these results suggest that substrates in vivo may not exert their effects on axon guidance principally via relative adhesiveness.

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Influence of substrate on the distribution of calcium channels in identified leech neurons in culture.

Cited by 44 publications

References 10 publications

Electrical fields, nerve growth and nerve regeneration

Electrical fields, nerve growth and nerve regeneration

Properties of an identified dopamine‐containing neurone in culture from the snail Helisoma

Neurite growth on different substrates: permissive versus instructive influences and the role of adhesive strength

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