Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use

Hunter, A. R.; Archer, Charles W.; Walker, Peter S.; Blunn, GW

doi:10.1016/0142-9612(95)93256-d

Cited by 264 publications

(177 citation statements)

References 12 publications

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“…This incorporation causes the preosteoblasts to be stimulated into adult osteoblasts and increases proliferation of bone cells [60]. The crystal HA surface is required for faster osteoblast proliferation which is essential for stimulating the process of bone healing [61,62]. The HA incorporation improves the layer hydrophilicity compared to the SS316L, Ti6Al4V, and pure MFI coating.…”

Section: Drug-delivering Biomaterialsmentioning

confidence: 99%

Zeolite-based biomaterials for biomedical application: A review

Purnomo

Setyarini

Sulistyaningsih

2018

AIP Conference Proceedings

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Abstract. This review focuses on the latest reports of the use of zeolites, natural as well as synthetic, as biomedical materials. Discussion on biocompatibility, non-toxicity, zeolite for drug delivery materials, zeolite for bone implants and implant coatings, and the mechanical properties of polymer-zeolite composites are included. The section on the interaction between body tissues and zeolite-based biomaterials focuses on their biocompatibility, non-toxicity, and bioactivity. The next section concerns drug delivering biomaterials. Including delivery of a controlled drug, ionic removal, and synthesized drug delivering materials. The final section concern bone graft substitutes and implant-coating to improve corrosion resistance and osteointegration. The implant material system discussed in a further section is an investigation of the mechanical properties of high-density polyethylene(HDPE)-zeolite composites developed as skull bone implant materials.

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Section: Drug-delivering Biomaterialsmentioning

confidence: 99%

Zeolite-based biomaterials for biomedical application: A review

Purnomo

Setyarini

Sulistyaningsih

2018

AIP Conference Proceedings

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“…2 is not frequently measured, especially the early attachment phase characterized by t 1/2 and %I max, because cell-enumeration protocols are quite labor intensive (there are a variety of cell-enumeration methods available including dye techniques [67][68][69][70][71][72][73][74], autoradiography [75], light and electron microscopy [76][77][78], Coulter counting [79], hemocytometry [80], spectrometry [81,82], nuclei number [83], total DNA [84], total protein concentration [78,85] that may or may not give similar results, depending on cell number and specific experimental conditions). Instead, a variety of experimental shortcuts are taken, such as measuring attached-cell-number after some arbitrary cell-surface contact time [86][87][88][89].…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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“…A further drawback of optimizing PEEK for medical implants is the optical imaging of cells on PEEK surfaces due to auto-fl uorescence (9,10) . The detailed investigation of the fl uorescence of commercially available PEEK fi lms (VICTREX) and pure PEEK revealed that the broadband auto-fl uorescence is an inherent material property and not caused by any additive.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of PEEK substrates, the application of fl uorescence microscopy is critical because of the strong auto-fl uorescence of the polymeric material. Hunter et al (9) , investigated the attachment and proliferation of osteoblasts and fi broblasts on biomaterials for orthopedic use, and explicitly excluded PEEK from the immuno-fl uorescence study due to the prominent auto-fl uorescence of the material. To overcome the problem, Briem et al (10) replaced the immuno-fl uorescence stains with Giemsa stains when investigating the response of primary fi broblasts and osteoblasts to plasma-treated PEEK.…”

Section: Introductionmentioning

confidence: 99%

Nanostructuring polyetheretherketone for medical implants

Althaus¹,

Padeste

Köser

et al. 2012

European Journal of Nanomedicine

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Surface roughness is a vital factor for medical implants since the cells of the surrounding tissue interact with the underlying substrate on the micro-and nanometer scales. In order to improve the surface morphology of implants, appropriate large-area micro-and nanostructuring techniques have to be identifi ed being applicable to irregularly shaped structures. We demonstrate that plasma treatments of polyetheretherketone (PEEK) thin fi lms produce nanostructured surfaces in a reproducible manner. They are easily tailored by varying plasma intensity using oxygen and ammonia as process gases. It was observed that roughness and nanostructure density linearly depend on plasma intensity. Oxygen plasma turned out to exhibit a stronger effect compared to ammonia plasma at the same processing conditions. For cell interaction studies, the mean size of the nanostructures was intentionally varied between 10 nm and 100 nm. In vitro experiments revealed that human mesenchymal stem cells (hMSC) adhere inhomogenously on untreated PEEK fi lms, but the plasma treatment with oxygen or ammonia allows the hMSC to adhere and proliferate. Fluorescence microscopy of the cells on the PEEK fi lms turned out to be diffi cult because of the strong auto-fl uorescence of the PEEK substrate. Stains including the whole cell vital stain Calcein-AM allowed cell morphology studies on plasma-treated PEEK fi lms. In the case of the analysis of cell compartments such as the actin cytoskeleton, confocal laser scanning microscopy (CLSM) was successfully applied.

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Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use

Cited by 264 publications

References 12 publications

Zeolite-based biomaterials for biomedical application: A review

Zeolite-based biomaterials for biomedical application: A review

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Nanostructuring polyetheretherketone for medical implants

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