Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells

Li, Xuejun; Liu, Fei; Zhao, Li; Ye, Ningrong; Huang, Chunhai; Yuan, Xianrui

doi:10.1016/j.ijbiomac.2014.02.019

Cited by 29 publications

(11 citation statements)

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“…Before our study, it was reported that AM had anti-inflammatory activities in several studies 2 , 4 and even tumor suppressive effect in various cancer treatment such as lung cancer, advanced colon cancer and brain glioma. 5 – 7 , 31 Though not touched in the current study, significant STAT3 inactivation as well as NF-κB repression of AM can also be clue to the prevention of CAC, if we followed up colitis model for longer time. Since carcinogenic effect might be caused by chronic inflammation because inflammation caused mutation or instability of genome, 28 the significant anti-inflammatory actions of AM and/or TH can exert both the preventive effect of IBD relapse and possibly prevention of CAC.…”

Section: Discussionmentioning

confidence: 83%

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

et al. 2017

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Background/AimsIn inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of Atractylodes macrocephala (AM) or Taraxacum herba (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration.MethodsMurine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge. Acute colitis was induced with 7 days of dextran sulfate sodium (DSS) in male C57BL/6 mice, and extracts of AM and TH were administered for 2 weeks before DSS administration.ResultsIn vitro studies demonstrated that AM or TH treatment reduced LPS-induced COX-2 and tumor necrosis factor-α mRNA levels but increased heme oxygenase-1 (HO-1). Oral preadministration of AM and TH rescued mice from DSS-induced colitis by inhibiting inflammatory mediators via inactivated extracellular signal regulated kinase and repressed nuclear factor κB and signal transducer and activator of transcription 3, but the effect was weaker for sulfasalazine than that for the extracts. Anti-inflammatory activities occurred via the inhibition of macrophage and T lymphocyte infiltrations. Unlike sulfasalazine, which did not induce HO-1, TH extracts afforded significant HO-1 induction.ConclusionsBecause the AM or TH extracts were far superior in preventing DSS-induced colitis than sulfasalazine, AM or TH extracts can be considered natural agents that can prevent IBD relapse.

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Section: Discussionmentioning

confidence: 83%

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

et al. 2017

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“…There are two main apoptosis types including the extrinsic apoptosis, i.e., death receptor pathway, and the intrinsic apoptosis, i.e., mitochondrial pathway (Fan et al, 2016;Huang et al, 2015). It was already confirmed that the downregulation of the mitochondrial membrane potential (MMP) is the first step in mitochondrial apoptosis, which results in the permeabilization of the mitochondrial membrane and release of Cytochrome C from the mitochondria into the cytoplasm (Chen et al, 2018;Li et al, 2014). To identify whether 9za-mediated cell apoptosis was initiated by the mitochondrial pathway in NSCLC cells, JC-1 staining assay was adopted to detect MMP (Xie et al, 2018).…”

Section: Za Induces Mitochondria-mediated Apoptosis In Nsclc Cellsmentioning

confidence: 99%

A novel dual MEK/PDK1 inhibitor 9za retards the cell cycle at G₀/G₁ phase and induces mitochondrial apoptosis in non-small cell lung cancer cells

Liu

Chen

et al. 2020

PeerJ

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Background A novel dual MEK/PDK1 inhibitor named 9za has been synthesized by our research team. Preliminary study showed that 9za possessed potent cytotoxicity and proapoptosis in non-small cell lung cancer (NSCLC) cells. Nevertheless, the precise underlying mechanism is vague. Methods In this work, we adopted the MTT assay, the Cell Cycle Detection Kit, and the JC-1 staining assay to detect the cell viability, the cell cycle distribution and the mitochondrial membrane potential (MMP), respectively. Cell apoptosis was measured by the morphology observation under a light microscope, Annexin V-FITC/propidium iodide (PI) apoptosis detection and the colorimetric TUNEL assay. Western blot was used to monitor the cell cycle-, apoptosis-related proteins and relevant proteins involved in the signaling pathways. Results The MTT assay demonstrated that 9za sharply decreased the viability of NSCLC cells. Cell cycle analysis revealed that low concentrations of 9za arrested the cell cycle at the G0/G1 phase , which was further confirmed by the decreased levels of Cyclin D1, cyclin-dependent kinase 4 (CDK4) and cyclin-dependent kinase 6 (CDK6). Additionally, morphological observations, Annexin V-FITC/propidium iodide (PI) apoptosis analysis and TUNEL assays indicated that high concentrations of 9za induced cell apoptosis. Furthermore, the JC-1 staining assay revealed that the mitochondrial membrane potential was downregulated following 9za exposure. Western blot also showed that 9za markedly decreased the expression levels of total Bcl-2, Cytochrome C in the mitochondria and BCL2 associated X (BAX) in the cytoplasm. However, the levels of BAX in the mitochondria, Cytochrome C in the cytoplasm, active caspase-9, active caspase-3 and cleaved–PARP showed the opposite changes. Moreover, the dose-dependent decreased phosphorylation levels of PDK1, protein kinase B (Akt), MEK and extracellular signal regulated kinase 1/2 (ERK1/2) after 9za treatment verified that 9za was indeed a dual MEK/PDK1 inhibitor, as we expected. Compared with a single MEK inhibitor PD0325901 or a single PDK1 inhibitor BX517, the dual MEK/PDK1 inhibitor 9za could strengthen the cytotoxic and proapoptotic effect, indicating that the double blocking of the MEK and PDK1 signaling pathways plays stronger cell growth inhibition and apoptosis induction roles than the single blocking of the MEK or PDK1 signaling pathway in NSCLC cells. Our work elucidated the molecular mechanisms for 9za as a novel drug candidate against NSCLC.

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“…It is also popularly used as heath cultivating food. There are a large number of natural compounds that extracted from Atractylodes macrocephala , such as polysaccharides, sesquiterpenoids and volatile oils [ 13 ]. AT-II as the major sesquiterpenoids isolated from the dried Rhizome of Atractylodes macrocephala shows a wide range of biological and pharmacological activities, for example, against insomnia and anxiety, neuroprotective, platelet activation and anti-cancer effect [ 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Atractylenolide II Inhibits Proliferation, Motility and Induces Apoptosis in Human Gastric Carcinoma Cell Lines HGC-27 and AGS

Shen

Hongdan

2017

Molecules

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Atractylenolide II (AT-II) exhibits several biological and pharmacological functions, especially anti-cancer activity as the major sesquiterpene lactones isolated from Atractylodes macrocephala (also named Baizhu in Chinese). However, the effects and mechanisms of AT-II on human gastric cancer remain unclear. Cell Counting Kit-8 (CCK-8) assay, morphological changes, flow cytometry, wound healing assay and Western blot analysis were used to investigate the effects of AT-II on cell proliferation, apoptosis and motility of human gastric carcinoma cell lines HGC-27 and AGS. Our results indicated that AT-II could significantly inhibit cell proliferation, motility and induce apoptosis in a dose and time-dependent manner. Western blot analysis showed that the expression level of Bax was upregulated and the expression levels of B-cell lymphoma-2 (Bcl-2), phosphorylated-protein kinase B (p-Akt) and phosphorylated-ERK (p-ERK) were downregulated compared to control group. In conclusion, the findings suggested that AT-II exerted significant anti-tumor effects on gastric carcinoma cells by modulating Akt/ERK signaling pathway, which might shed light on therapy of gastric carcinoma.

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Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells

Cited by 29 publications

References 27 publications

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

A novel dual MEK/PDK1 inhibitor 9za retards the cell cycle at G₀/G₁ phase and induces mitochondrial apoptosis in non-small cell lung cancer cells

Atractylenolide II Inhibits Proliferation, Motility and Induces Apoptosis in Human Gastric Carcinoma Cell Lines HGC-27 and AGS

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Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells

Cited by 29 publications

References 27 publications

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

A novel dual MEK/PDK1 inhibitor 9za retards the cell cycle at G0/G1 phase and induces mitochondrial apoptosis in non-small cell lung cancer cells

Atractylenolide II Inhibits Proliferation, Motility and Induces Apoptosis in Human Gastric Carcinoma Cell Lines HGC-27 and AGS

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A novel dual MEK/PDK1 inhibitor 9za retards the cell cycle at G₀/G₁ phase and induces mitochondrial apoptosis in non-small cell lung cancer cells