ATR FTIR Spectoscopy of Aqueous Cell Cultures v2

Spahiu, Emrulla; Dogan, Senol; Schnauß, Jörg; Gürsel, Mayda; Severcan, Feride

doi:10.17504/protocols.io.bdidi4a6

Cited by 2 publications

(1 citation statement)

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“…[53][54][55]. In the range of 3200 − 2800 cm − 1 symmetrical and asymmetrical expansions, O-H expansions, N-H expansions and vibrations are determined [56]. The hydroxyl (OH) group's peak at 1626 cm − 1 [52] and a peak at 3214 cm − 1 appertains the N-H groups.…”

Section: Thermal Behaviormentioning

confidence: 99%

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method 

Özkan

Türk

Çelik

2021

Preprint

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The present research demonstrates the synthesis and characterization of LaMnO3 perovskite powders using the sol-gel technique for dye-sensitive solar cell applications. With this respect, transparent solutions were prepared from La and Mn based precursors, distilled water and citric acid monohydrate. Ammonium hydroxide was incorporated into the La-Mn solution in order to neutralize/precipitate at 24oC for 1 hour in the air. The solution was allowed to evaporate on a hot plate device at 90 °C in the air. The obtained solutions were dried at 90 oC for 24 hours to form a xerogel structure, dried at 200 oC for 2 hours and consequently annealed at 500 and 850 oC for 2 hours in the air. Thermal, structural, microstructural, optical and magnetic properties of the powders were characterized through differential thermal analysis-thermogravimetry (DTA-TG), Fourier transform infrared (FTIR), X-ray diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), scanning electron microscopy (SEM), Malvern zeta sizer (PSD), UV-vis spectrometer and vibrating sample magnetometer (VSM). The obtained results indicate promise, especially the low band range, that LaMnO3 powders can be used in dye-sensitized solar cells and can positively affect performance and efficiency.

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Section: Thermal Behaviormentioning

confidence: 99%

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method 

Özkan

Türk

Çelik

2021

Preprint

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Activation of Macrophages by CpG DNA and LPS: an FTIR Spectroscopic Study

Spahiu

Dogan

Schnauß

et al. 2020

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The innate immune response triggered by CpG DNA can improve host survival following pathogen challenge. Whether CpG ODN-mediated immune activation leads to global molecular changes in cells that are detectable by FTIR spectroscopy is currently unknown. Here, we used Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) spectroscopy to monitor the molecular changes in murine macrophage RAW 264.7 cells upon activation with CpG DNA and lipopolysaccharide (LPS). By PCA analysis, we identified the sources of variation to follow with detailed spectral analysis. CpG DNA and LPS treatment increase the total nucleic acid concentration from the early periods post-activation, and DNA synthesis follows RNA synthesis. RNA-specific peak shows the activation state of macrophages in early periods post-treatment. CpG DNA and LPS result in an initial rapid increase in the total protein concentration, leveling off two hours post-activation. Both activated groups increase the concentration of fatty acids, triglycerides, and cholesterol, pointing out to a shared synthesis pathway and de novo lipogenesis. This study, for the first time, demonstrates the use of FTIR spectroscopy as an independent modality to monitor the activation dynamics of murine macrophages upon activation with CpG DNA and LPS.

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ATR FTIR Spectoscopy of Aqueous Cell Cultures v2

Abstract: ATR-FTIR Spectroscopy of aqueous cell culture samples is explained step-by-step. Infrared spectra acquisition, processing and analysis is included briefly.

Cited by 2 publications

References 0 publications

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method

Activation of Macrophages by CpG DNA and LPS: an FTIR Spectroscopic Study

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ATR FTIR Spectoscopy of Aqueous Cell Cultures v2

Abstract: ATR-FTIR Spectroscopy of aqueous cell culture samples is explained step-by-step. Infrared spectra acquisition, processing and analysis is included briefly.

Cited by 2 publications

References 0 publications

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel&nbsp;Method&nbsp;

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel&nbsp;Method&nbsp;

Activation of Macrophages by CpG DNA and LPS: an FTIR Spectroscopic Study

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Product

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Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method

Synthesis and Characterizations of LaMnO3 Perovskite Powders Using Sol-Gel Method