ATP Sulphurylase and<i>O</i>-Acetylserine Sulphydrylase in Isolated Mesophyll Protoplasts and Bundle Sheath Strands of S-deprived Maize Leaves

Passera, C.; Ghisi, R.

doi:10.1093/jxb/33.3.432

Cited by 41 publications

(29 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with the results ofGerwick et al (8) who detected only 2.4% of ATP sulfurylase in the mesophyll cells of Z. mays. In contrast, Passera and Ghisi (14) found 25 to 30% of total ATP sulfurylase activity in the mesophyll cells of Z. mays cv XL 72 A. The assay of marker enzymes for each cell type was not reported in the study.…”

Section: Discussionmentioning

confidence: 65%

“…Recently, a predominant localization ofenzymes ofassimilatory sulfate reduction in the bundle sheath cells was proposed (7,8,14). This pathway begins with the formation ofAPS2 from ATP and S042-via ATP sulfurylase (EC 2.7.7.4) (1,18 Kolibri) were imbibed for 24 h in aerated tap water at room temperature and then transferred to moist filter paper.…”

Section: Introductionmentioning

confidence: 99%

“…C4 plants are characterized by an intercellular compartmentation of CO2 and NO3-assimilation between mesophyll and bundle sheath cells (2,3,6,16). Recently, a predominant localization ofenzymes ofassimilatory sulfate reduction in the bundle sheath cells was proposed (7,8,14). This pathway begins with the formation ofAPS2 from ATP and S042-via ATP sulfurylase (EC 2.7.7.4) (1,18).…”

mentioning

confidence: 99%

“…In variety XL 72 A of Zea mays L., about 75% of total ATP sulfurylase was detected in this type of cell (14). Organic thiosulfate reductase/sulfite reductase were 2 to 3 times higher on a protein basis in bundle sheath extracts than in mesophyll extracts of crabgrass leaves (7).…”

mentioning

confidence: 99%

“…Organic thiosulfate reductase/sulfite reductase were 2 to 3 times higher on a protein basis in bundle sheath extracts than in mesophyll extracts of crabgrass leaves (7). In maize (cv XL 72 A), OASSase activity was found predominantly in the bundle sheath cells (14). Collec For the enzymic isolation procedure, incubation in the enzyme solution was 2.5 h in the light.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Intercellular Localization of Assimilatory Sulfate Reduction in Leaves of Zea mays and Triticum aestivum

Schmutz¹,

Brunold²

1984

Plant Physiol.

View full text Add to dashboard Cite

The intercellular distribution ofassimilatory sulfate reduction enzymes between mesophyll and bundle sheath cells was analyzed in maize (Zea mays L.) and wheat (Triticum aestivum L.) leaves. In maize, a C4 plant, 96 to 100% of adenosine 5'-phosphosulfate sulfotransferase and 92 to 100% of ATP sulfurylase activity (EC 2.7.7A) was detected in the bundle sheath cells. Sulfite reductase (EC 1.8.7.1) and O-acetyl-L-serine sulfhydrylase (EC 4.2.99.8) were found in both bundle sheath and mesophyll cell types. In wheat, a C3 species, ATP sulfurylase and adenosine 5'-phosphosulfate sulfotransferase were found at equivalent activities in both mesophyll and bundle sheath cells. Leaves of etiolated maize plants contained appreciable ATP sulfurylase activity but only trace adenosine 5'-phosphosulfate sulfotransferase activity. Both enzyme activities increased in the bundle sheath cells during greening but remained at negligible levels in mesophyll cells. In leaves of maize grown without addition of a sulfur source for 12 d, the specific activity of adenosine 5'-phosphosulfate sulfotransferase and ATP sulfurylase in the bundle sheath cells was higher than in the controls. In the mesophyll cells, however, both enzyme activities remained undetectable. The intercellular distribution of enzymes would indicate that the first two steps of sulfur assimilation are restricted to the bundle sheath cells of C4 plants, and this restriction is independent of ontogeny and the sulfur nutritional status of the plants.C4 plants are characterized by an intercellular compartmentation of CO2 and NO3-assimilation between mesophyll and bundle sheath cells (2,3,6,16). Recently, a predominant localization ofenzymes ofassimilatory sulfate reduction in the bundle sheath cells was proposed (7,8,14). This pathway begins with the formation ofAPS2 from ATP and S042-via ATP sulfurylase (EC 2.7.7.4) (1,18 Kolibri) were imbibed for 24 h in aerated tap water at room temperature and then transferred to moist filter paper. After 3 d in the dark, the seedlings were cultivated on quartz sand watered regularly with nutrient solution (23). Nutrient solution deficient in S was composed by replacing the SO42-salts by equimolar amounts of the corresponding Cl-salts. The plants were grown at 26°C, with a RH of 40% and a light intensity of 46 ,uE m-2 s-' provided by incandescent lamps (Philips TL 40 w).Cell Isolation. Mesophyll cells and bundle sheath strands were obtained either by a mechanical or by an enzymic isolation procedure. For both procedures, leaves were cut diagonally in approximately 1-mm-wide strips with a razor blade and then transferred to a medium for protoplast isolation according to Mills and Joy (12), containing 2% cellulase and 2% pectinase. Ten ml of medium were used per g leafstrips. The plant material was infiltrated by applying a vacuum. The incubation time was 15 min in the light for the mechanical procedure. The plant material was then homogenized using a Servall Omni Mixer (Ivan Sorvall, Norwalk, CT) for 10 s at 100 v (6,700 rpm) an...

show abstract

Section: Discussionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%