ATP sulfurylase activity of sulfate-reducing bacteria from various ecotopes

Abstract

Sulfate-reducing bacteria (SRB) are widespread in various ecotopes despite their growth and enzymatic features not compared. In this study, the enzymatic parameters of ATP sulfurylase in cell-free extracts of sulfate-reducing bacteria isolated from various ecotopes such as soils, corrosion products and human large intestine were determined. Comparative analysis of both enzyme characteristics and growth parameters were carried out and similar research has not been reported yet. The initial and maximum rates of enzymatic reaction catalyzed by ATP sulfurylase were significantly different (p < 0.05) in the bacterial strains isolated from various environmental ecotopes. The specific activity of this enzyme in sulfate-reducing bacteria was determined for corrosive and intestinal strains 0.98-1.56 and 0.98-2.26 U × mg −1 protein, respectively. The Michaelis constants were 1.55-2.29 mM for corrosive and 2.93-3.13 mM for intestinal strains and the affinity range were demonstrated. Based on cluster analysis, the parameters of physiological and biochemical characteristics of sulfate-reducing bacteria from different ecotopes are divided into 3 clusters corresponding to the location of their isolation (soils, heating systems and human intestine). Understanding the enzymatic parameters of the initial stages of sulfate consumption in the process of dissimilatory sulfate reduction will allow the development of effective methods for controlling the production of toxic metabolites, including hydrogen sulfide.

Hydrogen sulfide toxicity in the gut environment: Meta-analysis of sulfate-reducing and lactic acid bacteria in inflammatory processes

“…Sulfate is activated by ATP sulfurylase, a single ATP molecule is consumed and adenosine 5́-phosphosulfate (APS) is formed ( Fig. 5 ) [73] , [74] .

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“…Due to this, redox potential has to be increased. It is done by ATP sulfurylase, while consuming ATP [74] : SO 4 2− + ATP ↔ APS + PP i …”

Adenosine-5′-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

“…For intestinal strains of SRB, similar results were obtained for the first step of sulfate-reduction pathway, i.e., ATP sulfurylase. We discussed that this decrease in the maximum rate of the enzyme could show the inhibition of the enzymatic process of sulfate accumulation in the bacterial cells at the end of the reaction [39]. Therefore, it is possible that, in addition to competition for substrates with methanogens by the intestinal SRB strains, inhibition of the final stages of sulfate reduction may occur [71,72].…”

“…The sulfate reduction inhibition effects of the initial stage in SRB isolated from intestinal strains have also been shown. The process can be recognized as the unusual adaptation of SRB to the intestinal environment [ 39 ].…”

Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

“…[ 7 , 12 , 34 , 35 ]. The studies emphasize connections between the presence of SRB in the intestines and the prevalence of ailments, such as cholecystitis, brain abscesses and abdominal cavity ulcerative enterocolitis [ 7 , 11 , 36 , 37 ]. SRB are not the only organisms of the intestine producing H 2 S. Although SRB isolates coming from the intestine of humans have been reported to belong almost exclusively to Desulfovibrio sp., other genera are also part of the SRB microbiota: Desulfotomaculum, Desulfobulbus, Desulfomicrobium, Desulfomonas, Desulfonema [ 12 , 28 ].…”