ATP Mimics pH-Dependent Dual Peroxidase-Catalase Activities Driving H
            <sub>2</sub>
            O
            <sub>2</sub>
            Decomposition

Shi, Ying; Tang, Menghuan; Sun, Chaoqun; Pan, Yadi; Liu, Li; Long, Yijuan; Zheng, Huzhi

doi:10.31635/ccschem.019.20190017

Cited by 25 publications

(17 citation statements)

References 44 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, there were differences between extracellular and intracellular ATP levels, as well as PANX1 expression. We suggest that excessive extracellular H 2 O 2 levels in the medium of the in vitro model (Fig 1A) could cause ATP hydrolysis, and therefore reduces its extracellular levels, which is consistent with previous findings [47]. Taken together, we confirmed that bleaching gels can cause pain transmission of DPSCs through intracellular ROS.…”

Section: Discussionsupporting

confidence: 91%

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

et al. 2021

View full text Add to dashboard Cite

Background Bleaching is widely accepted for improving the appearance of discolored teeth; however, patient compliance is affected by bleaching-related complications, especially bleaching sensitivity. This study aimed to investigate the role of reactive oxygen species (ROS) in cytotoxicity and pain conduction activated by experimental tooth bleaching. Methods Dental pulp stem cells with or without N-acetyl-L-cysteine (NAC), an ROS scavenger, were cultured on the dentin side of the enamel/dentin disc. Subsequently, 15% (90 min) and 40% (30 min) bleaching gels were painted on the enamel surface. Cell viability, intracellular ROS, Ca2+, adenosine triphosphate (ATP), and extracellular ATP levels were evaluated using the Cell Counting Kit-8 assay, 2’,7’-dichlorodihydrofluorescein diacetate, CellROX, fura-3AM fluorescence assay, and ATP measurement kit. The rat incisor model was used to evaluate in vivo effects after 0, 1, 3, 7, and 30 days of bleaching. Changes in gene and protein expression of interleukin 6 (IL-6), tumor necrosis factor-alpha (TNFα), transient receptor potential ankyrin 1 (TRPA1), and Pannexin1 (PANX1) in dental pulp stem cells and pulp tissue were detected through RT-PCR, western blotting, and immunofluorescence. Results The bleaching gel suppressed dental pulp stem cell viability and extracellular ATP levels and increased intracellular ROS, Ca2+, and intracellular ATP levels. The mRNA and protein expression of IL-6, TNFα, TRPA1, and PANX1 were up-regulated in vitro and in vivo. Furthermore, the 40% gel had a stronger effect than the 15% gel, and NAC ameliorated the gel effects. Conclusions Our findings suggest that bleaching gels induce cytotoxicity and pain conduction in dental pulp stem cells via intracellular ROS, which may provide a potential therapeutic target for alleviating tooth bleaching nociception.

show abstract

Section: Discussionsupporting

confidence: 91%

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The mismatched base pairs could add flexibility to the duplex, and the increased flexibility allowed for the formation of circular assemblies, therefore inhibiting the assembly process. [ 79 ] These results indicated that the lengths of sticky ends play a key role in the mechanical properties of self‐assembled hydrogels. In another work, Liu et al further demonstrated that the thermal stability of gels increased with the increased length of sticky ends, while the base mismatch greatly reduced the thermal stability of gels.…”

Section: Methods To Regulate and Control The Mechanical Properties In...mentioning

confidence: 98%

“…Liu et al explained this phenomenon in another work. [ 79 ] When the monomer length is too short, the resulted duplex is not sufficiently stable at room temperature; when the monomer length is too long, the hairpin structure within the molecule is more stable than duplex, and the formation of hairpin results in self‐termination during the polymerization process. The above investigation leads to the conclusion that adjusting the length of sticky ends is to adjust the energy barrier required for the deconstruction of DNA hydrogel, thus affecting its stability and further the mechanical properties of the hydrogel.…”

Section: Methods To Regulate and Control The Mechanical Properties In...mentioning

confidence: 99%

DNA Hydrogels in the Perspective of Mechanical Properties

Cao

Xie

Song

2022

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Tailoring the mechanical properties has always been a key to the field of hydrogels in terms of different applications. Particularly, DNA hydrogels offer an unambiguous way to precisely tune the mechanical properties, largely on account of their programmable sequences, abundant responding toolbox, and various ligation approaches. In this review, DNA hydrogels from the perspective of mechanical properties, from a synthetic standpoint to different applications, are introduced. The relationship between the structure and their mechanical properties in DNA hydrogels and the methods of regulating the mechanical properties of DNA hydrogels are specifically summarized. Furthermore, several recent applications of DNA hydrogels in relation to their mechanical properties are discussed. Benefiting from the tunability and flexibility, rational design of mechanical properties in DNA hydrogels provided unheralded interest from fundamental science to extensive applications.

show abstract

“…We speculate that the reason is that the body may have a "compensatory" response, that is, the THz photons are energy-supplying during the illumination, so the cells no longer need to synthesize large amounts of ATP, resulting in reduced ATP release. We also Previous studies have reported that ATP could play the role of peroxidase in mitochondria to remove excess reactive oxygen species (ROS) and ruled out the possibility that ATP promoted the decomposition of ROS by releasing energy [30]. We therefore tested preprint (which was not certified by peer review) is the author/funder.…”

Section: Resultsmentioning

confidence: 99%

A new means of energy supply driven by terahertz photons recovers related neural activity

Tan

2022

Preprint

View full text Add to dashboard Cite

Continuous and efficient energy capture represents a long-sought dream of mankind. The brain is a major energy consuming organ, an adult brain accounts for about 2% of the body weight but consumes about 20% of the body's energy. However, it is still unclear how the brain achieve efficient use of energy. Here, using nerve cells as test subjects, we found that THz photons with a specific frequency can effectively restore the reduced frequency of action potentials caused by inadequate adenosine 5'-triphosphate (ATP) supply, which has been demonstrated as a novel mode of energy supply, present photons emission at a particular frequency from the breaking of the ATP phosphate bond. This energy supply mechanism may play a key biophysical basis for explaining how the body efficiently obtain energy, because the quantized chemical reactions could have a high energy efficiency and ultrahigh selectivity compared with the traditional thermochemistry and photochemistry.

show abstract

ATP Mimics pH-Dependent Dual Peroxidase-Catalase Activities Driving H ₂ O ₂ Decomposition

Cited by 25 publications

References 44 publications

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

DNA Hydrogels in the Perspective of Mechanical Properties

A new means of energy supply driven by terahertz photons recovers related neural activity

Contact Info

Product

Resources

About

ATP Mimics pH-Dependent Dual Peroxidase-Catalase Activities Driving H 2 O 2 Decomposition

Cited by 25 publications

References 44 publications

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

DNA Hydrogels in the Perspective of Mechanical Properties

A new means of energy supply driven by terahertz photons recovers related neural activity

Contact Info

Product

Resources

About

ATP Mimics pH-Dependent Dual Peroxidase-Catalase Activities Driving H ₂ O ₂ Decomposition