ATP measurement in sheep rumen digesta using dimethyl sulfoxide as an extraction reagent

Komisarczuk, Sylvie; Durand, Michelle; Hannequart, Geneviève

doi:10.1051/rnd:19840707

Cited by 9 publications

(4 citation statements)

References 5 publications

(7 reference statements)

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“…However, there was high interindividual variation and this was not consistent with the changes in SCFA with sampling time. Large interindividual variations in ATP concentration of rumen digesta have also been reported (Erfle et al 1981 ; Komisarczuk et al 1984). Thus, in contrast to previous results obtained in rats or pigs (Bach Knudsen et al 1984, the validity of this indicator for the young rabbit remains to be confirmed.…”

Section: Discussionmentioning

confidence: 86%

“…SCFA were measured by gas chromatography (CP9000, Chrompack, Middelburg, The Netherlands) by the method of Jouany (1982) adapted to a semi-capillary column and NH, concentrations were measured by the technique of Weatherburn (1967) with an autoanalyser (Technicon, Domont, France). The concentration of ATP in caecal digesta was estimated by the luciferinluciferase (EC 1.13.12.7) method (McElroy, 1947;Komisarczuk et al 1984) (ATP dosing kit, BioOrbit, Turku, Finland). Calculations Apparent faecal digestibility was calculated from individual and daily total collections of faeces performed during six consecutive days.…”

Section: Biochemical Analysesmentioning

confidence: 99%

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Consequences of reduced fibre intake on digestion, rate of passage and caecal microbial activity in the young rabbit

Bellier¹,

Gidenne²

1996

Br J Nutr

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The present work was undertaken to study in vivo fibre degradation, rate of passage and caecal fermentation activity (CFA) in the young rabbit (7 weeks old) receiving ad lib. a control (C) or a lowfibre (LF) diet (400 and 220 g neutral-detergent fibre (NDF)/kg respectively). As a consequence of the 50% reduction in the dietary fibre level, the voluntary food intake of the rabbits decreased by 25%, and the daily fibre intake was reduced by 60 % (26.7 and 10.8 g NDF/d for groups C and LF, respectively). In spite of a longer mean retention time of the fibre particles, the quantity of fibre digested daily was significantly lower (P < 001) for the LF than for the C group (4-0 and 7 8 g NDF/d respectively). The circadian distribution of the faecal excretion (as a percentage of the total DM output) did not differ between diets (P = 052) and no interaction was found (P = 0.96) between diet and time of excretion. Also, variables describing the CFA showed no interactions between diet (C or LF) and time of sampling (during caecotrophy or during hard faeces excretion). Our results indicated no direct relationship between the quantity of fibre digested and the total short-chain fatty acid concentration in the caecum, but the fermentation pattern indicated lower proportions of acetate for the LF diet. Higher levels of diaminopimelic acid (DAPA) and ATP were found for the LF diet associated with an improved dietary fibre digestibility, suggesting a higher microbial activity. However, this effect was balanced by a lower caecal digesta turnover rate and the microbial biomass output estimated through the faecal DAPA output did not vary significantly.

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Section: Discussionmentioning

confidence: 86%

Section: Biochemical Analysesmentioning

confidence: 99%

Consequences of reduced fibre intake on digestion, rate of passage and caecal microbial activity in the young rabbit

Bellier¹,

Gidenne²

1996

Br J Nutr

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“…Glycogen content of the cell suspension was assayed using a-amylo 1 4 , 1 4 glucosidase according to Roehring and Allred (31). ATP content of cells isolated from 2-d-old suckling pigs and incubated for 30 min with 2 mM glucose was measured by the luciferin-luciferase system after extraction with dimethyl sulfoxide (32). Galactokinase (EC 2.7.1.6) activity was determined in homogenates of enterocytes (n = 3) isolated from 0-d-old, 2-d-old suckling, 12-d-old, and postweaning animals, according to the method of Ballard (33).…”

Section: Animalsmentioning

confidence: 99%

Glucose, Galactose, and Glutamine Metabolism in Pig Isolated Enterocytes during Development

Darcy‐Vrillon¹,

Posho²,

Morel³

et al. 1994

Pediatr Res

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“…Samples were taken after a new equilibration period of 4 days, judged sufficient from the volatile fatty acid (VFA) production which stabilized. ATP concentrations were measured in the liquid phase of the vessels four times a day (just before and 2, 4 and 6 h after the feed bag was changed) and in the washed solid residues, as described by Komisarczuk, Durand and Hannequart (1984). One ml of liquid or 1 g of solid was treated with 9 ml of dimethylsulfoxide for ATP extraction and ATP was determined by bioluminescence.…”

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confidence: 99%

Effects of phosphorus deficiency on rumen microbial activity associated with the solid and liquid phases of a fermentor (Rusitec)

Komisarczuk¹,

Durand²,

Beaumatin³

et al. 1987

Reprod. Nutr. Dévelop.

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Summary. The rumen simulation technique (Rusitec) has been used to study the effects of phosphorus (P) deficiency on bacterial protein synthesis and chemical composition and on adenosine triphosphate (ATP) concentrations in the solid and liquid phases of fermentors. 16 g DM of a P-deficient mixed diet was put into each vessel daily and the vessels were infused with about 1 1 of a P-deficient (0 mg of P) or P-supplemented (120 mg of P/1) buffer. Two vessels per treatment were used, and during a second experimental period the deficient and supplemented buffers were interchanged to determine the effects of P repletion and depletion.The proportion of bacterial N directly incorporated from dietary amino acids or peptides was greater in solid-associated bacteria (SAB) than in liquid-associated bacteria (LAB) (46 vs 19 %). P deficiency increased this proportion in both bacterial populations. Protein synthesis associated with the solid phase represented about 30 % of the total protein synthesis in the system. P deficiency induced a marked decrease in microbial protein synthesis in both phases. Microbial yield declined by about 5 points (g of N/kg OMF) in P-deficient conditions. ATP concentrations were greatly reduced in both phases but P deficiency had no effect on protozoal numbers.The effects of P depletion during period II were similar to those of P deficiency in period I, and P repletion showed that the effects of P deficiency were almost entirely reversible.Introduction.

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ATP measurement in sheep rumen digesta using dimethyl sulfoxide as an extraction reagent

Abstract: Summary. Dimethyl sulfoxide (DMSO) Introduction.

Cited by 9 publications

References 5 publications

Consequences of reduced fibre intake on digestion, rate of passage and caecal microbial activity in the young rabbit

Consequences of reduced fibre intake on digestion, rate of passage and caecal microbial activity in the young rabbit

Glucose, Galactose, and Glutamine Metabolism in Pig Isolated Enterocytes during Development

Effects of phosphorus deficiency on rumen microbial activity associated with the solid and liquid phases of a fermentor (Rusitec)

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