ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor

Takeda, Michio; Kawamura, Tetsuya; Kobayashi, Mami; Endou, Hitoshi

doi:10.1080/15216549600201382

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…Functionally, ATP and uridine 5′-triphosphate (UTP), probably acting via P2Y 2 and/or P2Y 4 receptors, increased inositol 1,4,5-trisphosphate formation and activated the p38-stressactivated protein kinase cascade in rat renal mesangial cells [144,286,361]. Extracellular ATP increases [Ca 2+ ] i by release from intracellular stores, indicating mediation via P2Y receptors [121,282], although P2X receptor-mediated increase in [Ca 2+ ] i has also been claimed [311].…”

Section: Mesangial Cellsmentioning

confidence: 99%

Purinergic signalling in the kidney in health and disease

Burnstock

Evans

Bailey

2013

Purinergic Signalling

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The involvement of purinergic signalling in kidney physiology and pathophysiology is rapidly gaining recognition and this is a comprehensive review of early and recent publications in the field. Purinergic signalling involvement is described in several important intrarenal regulatory mechanisms, including tuboglomerular feedback, the autoregulatory response of the glomerular and extraglomerular microcirculation and the control of renin release. Furthermore, purinergic signalling influences water and electrolyte transport in all segments of the renal tubule. Reports about purine-and pyrimidine-mediated actions in diseases of the kidney, including polycystic kidney disease, nephritis, diabetes, hypertension and nephrotoxicant injury are covered and possible purinergic therapeutic strategies discussed.

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Section: Mesangial Cellsmentioning

confidence: 99%

Purinergic signalling in the kidney in health and disease

Burnstock

Evans

Bailey

2013

Purinergic Signalling

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“…Carmichael et al (1988),Ohigashi et al (1993),Nagy (1994),Guzman et al (1996),Capiod (1998), Dixon et al (2000,Che Ishikawa et al (1994), Takeda et al (1996,Huwiler et al (1997),Schulze-Lohoff et al (1998), Brown et al …”

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confidence: 99%

The birth and postnatal development of purinergic signalling

et al. 2010

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The purinergic signalling system is one of the most ancient and arguably the most widespread intercellular signalling system in living tissues. In this review we present a detailed account of the early developments and current status of purinergic signalling. We summarize the current knowledge on purinoceptors, their distribution and role in signal transduction in various tissues in physiological and pathophysiological conditions.

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“…PCR studies demonstrate message expression for P2X 2 , P2X 3 , P2X 4 , P2X 5 , P2X 7 , P2Y 1 , P2Y 2 , P2Y 4 , and P2Y 6 receptors (2,43,52). Functional and protein expression studies support the presence of P2X 7 , P2Y 1 , P2Y 2 , and P2Y 4 receptors by cultured rat mesangial cells with little or no published evidence for other receptor subtypes (12,27,47,52,54,60). In the current report, there is functional and molecular evidence supporting mouse mesangial cell expression of P2X 7 , P2Y 2 , and P2Y 4 receptors as is observed in the rat, plus the unique expression of P2X 2 and P2X 4 receptors.…”

Section: Discussionmentioning

confidence: 84%

P2 receptor regulation of [Ca²⁺]_iin cultured mouse mesangial cells

Rivera¹,

Zhang²,

Fuller³

et al. 2007

American Journal of Physiology-Renal Physiology

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Experiments were performed to establish the pharmacological profile of purinoceptors and to identify the signal transduction pathways responsible for increases in intracellular calcium concentration ([Ca(2+)](i)) for cultured mouse mesangial cells. Mouse mesangial cells were loaded with fura 2 and examined using fluorescent spectrophotometry. Basal [Ca(2+)](i) averaged 102 +/- 2 nM (n = 346). One hundred micromolar concentrations of ATP, ADP, 2',3'-(benzoyl-4-benzoyl)-ATP (BzATP), ATP-gamma-S, and UTP in normal Ca(2+) medium evoked peak increases in [Ca(2+)](i) of 866 +/- 111, 236 +/- 18, 316 +/- 26, 427 +/- 37, and 808 +/- 73 nM, respectively. UDP or 2-methylthio-ATP (2MeSATP) failed to elicit significant increases in [Ca(2+)](i), whereas identical concentrations of adenosine, AMP, and alpha,beta-methylene ATP (alpha,beta-MeATP) had no detectable effect on [Ca(2+)](i). Removal of Ca(2+) from the extracellular medium had no significant effect on the peak increase in [Ca(2+)](i) induced by ATP, ADP, BzATP, ATP-gamma-S, or UTP compared with normal Ca(2+); however, Ca(2+)-free conditions did accelerate the rate of decline in [Ca(2+)](i) in cells treated with ATP and UTP. [Ca(2+)](i) was unaffected by membrane depolarization with 143 mM KCl. Western blot analysis for P2 receptors revealed expression of P2X(2), P2X(4), P2X(7), P2Y(2), and P2Y(4) receptors. No evidence of P2X(1) and P2X(3) receptor expression was detected, whereas RT-PCR analysis reveals mRNA expression for P2X(1), P2X(2), P2X(3), P2X(4), P2X(7), P2Y(2), and P2Y(4) receptors. These data indicate that receptor-specific P2 receptor activation increases [Ca(2+)](i) by stimulating calcium influx from the extracellular medium and through mobilization of Ca(2+) from intracellular stores in cultured mouse mesangial cells.

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ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor

Cited by 6 publications

References 9 publications

Purinergic signalling in the kidney in health and disease

Purinergic signalling in the kidney in health and disease

The birth and postnatal development of purinergic signalling

P2 receptor regulation of [Ca²⁺]_iin cultured mouse mesangial cells

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ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor

Cited by 6 publications

References 9 publications

Purinergic signalling in the kidney in health and disease

Purinergic signalling in the kidney in health and disease

The birth and postnatal development of purinergic signalling

P2 receptor regulation of [Ca2+]iin cultured mouse mesangial cells

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P2 receptor regulation of [Ca²⁺]_iin cultured mouse mesangial cells