ATP effects on response of human erythrocyte membrane to high pressure

Yamaguchi, Takeo; Fukuzaki, Shunji

doi:10.2142/biophysico.16.0_158

Cited by 9 publications

(5 citation statements)

References 46 publications

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“…Therefore, an increase in the concentration of ATP could contribute through the operation of the Ca (2+)—ATPase to the restoration of normal calcium levels in red blood cells and a decrease in their aggregation. In addition, an increase in ATP content in erythrocytes leads to phosphorylation of spectrin, ankyrin and band 4.1 proteins, weakening protein–protein interactions and affecting cell plasticity in general [ 35 ]. 2,3-DPG reversibly interacts with cytoskeleton proteins [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

Deryugina

Danilova

Пичугин

et al. 2023

Life

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Molecular hydrogen has an anti-inflammatory and cardioprotective effect, which is associated with its antioxidant properties. Erythrocytes are subjected to oxidative stress in pathologies of the cardiovascular system, which is the cause of a violation of the gas transport function of blood and microcirculation. Therefore, our aim was to investigate the effects of H2 inhalation on the functional states of red blood cells (RBCs) in chronic heart failure (CHF) in rats. The markers of lipid peroxidation, antioxidant capacity, electrophoretic mobility of erythrocytes (EPM), aggregation, levels of adenosine triphosphate (ATP) and 2,3-diphosphoglyceric acid (2,3-DPG), hematological parameters were estimated in RBCs. An increase in EPM and a decrease in the level of aggregation were observed in groups with multiple and single H2 application. The orientation of lipoperoxidation processes in erythrocytes was combined with the dynamics of changes in oxidative processes in blood plasma, it was observed with both single and multiple exposures, although the severity of the changes was greater with multiple H2 inhalations. Probably, the antioxidant effects of molecular hydrogen mediate its metabolic action. Based on these data, we conclude the use of H2 improves microcirculation and oxygen transport function of blood and can be effective in the treatment of CHF.

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Section: Discussionmentioning

confidence: 99%

The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

Deryugina

Danilova

Пичугин

et al. 2023

Life

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“…For instance, pressure-induced hemolysis and hypotonic one are greatly affected by the bilayer-cytoskeleton interaction and the surface area-to-volume ratio, respectively. 9,[19][20][21] Particularly, much interesting information has been obtained from the analysis of pressure-induced hemolysis. 9,13,14,19,20) So, our atten-tion is focused on sterol effects on the membrane stability of erythrocytes under pressure.…”

Section: Discussionmentioning

confidence: 99%

“…When erythrocyte ghosts are incubated in a hypotonic buffer, cytoskeletal proteins such as spectrin are detached from the membrane. 17,19) Such detached proteins are released into the buffer. So, we examined the sterol effect on such protein detachment using a sterol-loaded erythrocyte membrane.…”

Section: Effects Of Sterols On Detachment Of Cytoskeletal Proteins From the Erythrocyte Membrane Under Hypotonic Buffermentioning

confidence: 99%

Importance of Cholesterol Side Chain in the Membrane Stability of Human Erythrocytes

Yamaguchi

Manaka

Ogura

et al. 2021

Biological & Pharmaceutical Bulletin

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Cholesterol suppresses the hemolysis and the detachment of cytoskeletal proteins from bilayer in the human erythrocyte membrane under stress conditions. However, there is little information on how cholesterol functions. So, examining the role of a short side chain of cholesterol, we used the plant sterols such as β-sitosterol and stigmasterol. Incorporation of sterols into the membrane using a sterol/methyl-βcyclodextrin complex was confirmed by the mass spectrometry. Hemolysis of human erythrocytes under high hydrostatic pressure (200 MPa) or hypotonic conditions was suppressed by cholesterol, but not by β-sitosterol and stigmasterol. Moreover, the bilayer-cytoskeleton interaction was also strengthened by cholesterol, but not by β-sitosterol and stigmasterol. Taken together, we suggest that the short side chain of cholesterol plays an important role in the membrane stability of human erythrocytes.

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“…The strengthening of the interaction between RBC membrane and the cytoskeleton upon ATP depletion increases the surface tension of the membrane (Betz et al, 2009 ). According to a recent study, when such RBCs are exposed to a high pressure (200 MPa), fragmentation and hemolysis are greatly inhibited (Yamaguchi and Fukuzaki, 2019 ). This could explain the low fragmentation sensitivity of metabolically depleted RBCs in this study.…”

Section: Discussionmentioning

confidence: 99%

A Novel Fragmentation Sensitivity Index Determines the Susceptibility of Red Blood Cells to Mechanical Trauma

et al. 2021

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Supraphysiological shear stresses (SSs) induce irreversible impairments of red blood cell (RBC) deformability, overstretching of RBC membrane, or fragmentation of RBCs that causes free hemoglobin to be released into plasma, which may lead to anemia. The magnitude and exposure tisme of the SSs are two critical parameters that determine the hemolytic threshold of a healthy RBC. However, impairments in the membrane stability of damaged cells reduce the hemolytic threshold and increase the susceptibility of the cell membrane to supraphysiological SSs, leading to cell fragmentation. The severity of the RBC fragmentation as a response to the mechanical damage and the critical SS levels causing fragmentation are not previously defined. In this study, we investigated the RBC mechanical damage in oxidative stress (OS) and metabolic depletion (MD) models by applying supraphysiological SSs up to 100 Pa by an ektacytometer (LORRCA MaxSis) and then assessed RBC deformability. Next, we examined hemolysis and measured RBC volume and count by Multisizer 3 Coulter Counter to evaluate RBC fragmentation. RBC deformability was significantly impaired in the range of 20–50 Pa in OS compared with healthy controls (p < 0.05). Hemolysis was detected at 90–100 Pa SS levels in MD and all applied SS levels in OS. Supraphysiological SSs increased RBC volume in both the damage models and the control group. The number of fragmented cells increased at 100 Pa SS in the control and MD and at all SS levels in OS, which was accompanied by hemolysis. Fragmentation sensitivity index increased at 50–100 Pa SS in the control, 100 Pa SS in MD, and at all SS levels in OS. Therefore, we propose RBC fragmentation as a novel sensitivity index for damaged RBCs experiencing a mechanical trauma before they undergo fragmentation. Our approach for the assessment of mechanical risk sensitivity by RBC fragmentation could facilitate the close monitoring of shear-mediated RBC response and provide an effective and accurate method for detecting RBC damage in mechanical circulatory assist devices used in routine clinical procedures.

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ATP effects on response of human erythrocyte membrane to high pressure

Cited by 9 publications

References 46 publications

The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

Importance of Cholesterol Side Chain in the Membrane Stability of Human Erythrocytes

A Novel Fragmentation Sensitivity Index Determines the Susceptibility of Red Blood Cells to Mechanical Trauma

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