The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure

Abstract

Molecular hydrogen has an anti-inflammatory and cardioprotective effect, which is associated with its antioxidant properties. Erythrocytes are subjected to oxidative stress in pathologies of the cardiovascular system, which is the cause of a violation of the gas transport function of blood and microcirculation. Therefore, our aim was to investigate the effects of H2 inhalation on the functional states of red blood cells (RBCs) in chronic heart failure (CHF) in rats. The markers of lipid peroxidation, antioxidant capacity, electrophoretic mobility of erythrocytes (EPM), aggregation, levels of adenosine triphosphate (ATP) and 2,3-diphosphoglyceric acid (2,3-DPG), hematological parameters were estimated in RBCs. An increase in EPM and a decrease in the level of aggregation were observed in groups with multiple and single H2 application. The orientation of lipoperoxidation processes in erythrocytes was combined with the dynamics of changes in oxidative processes in blood plasma, it was observed with both single and multiple exposures, although the severity of the changes was greater with multiple H2 inhalations. Probably, the antioxidant effects of molecular hydrogen mediate its metabolic action. Based on these data, we conclude the use of H2 improves microcirculation and oxygen transport function of blood and can be effective in the treatment of CHF.

Can Hydrogen Water Enhance Oxygen Saturation in Patients with Chronic Lung Disease? A Non-Randomized, Observational Pilot Study

“…However, this hypothetical is at odds with our study and previous investigations [ 7 , 10 ] in which hydrogen therapy was associated with increased oxygen levels. This might be explained by a recent study demonstrating that in rats with chronic heart failure, inhalation of molecular hydrogen led to increased erythrocyte ATP production [ 12 ] [9a]. The study also found increased levels of 2,3 bisphosphoglycerate, which resulted in improved microcirculation and oxygen transport function of the blood [ 12 ].…”

Alpha Hope, via Molecular Hydrogen and Pyrroloquinoline Quinone, Dose-Dependently Increases Nrf2 and PGC-1α Levels in C2C12 Myoblasts

“…These results may be partially explained by the favorable effects of molecular hydrogen on erythrocytes. For example, it was demonstrated that in rats, the administration of molecular hydrogen resulted in an increase in erythrocyte ATP production and levels of 2,3 bisphosphoglycerate, which consequently improved microcirculation and oxygen transport [48].…”