ATP dynamic regeneration strategy for enhancing co-production of glutathione and S-adenosylmethionine in Escherichia coli

Chen, Ya Wei; Liao, Yuan; Kong, Wei Zhen; Wang, Shu Han

doi:10.1007/s10529-020-02989-9

Cited by 13 publications

(12 citation statements)

References 14 publications

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“…coli. Similar results, stable intracellular ATP level through the fermentation and improved GSH (137.40%) and SAM (82.18%) coproduction, were achieved …”

Section: Genetic Circuits For Sensing and Dynamic Regulation Of Intra...mentioning

confidence: 99%

“…131 Artificial genetic circuits based on the ATP-sensing riboswitch ydaO motif were developed to control vhb and ptxD expression. Dynamic regulation of intracellular ATP pool to appropriate level improved SAM production by 55% 131 and 82.18%, 129 respectively, in different studies. The genetic circuits will be discussed in section 5.2.…”

Section: Cofactor Sam Atp and Nad(p)h Regeneration And Connectionsmentioning

confidence: 99%

“…Similar results, stable intracellular ATP level through the fermentation and improved GSH (137.40%) and SAM (82.18%) coproduction, were achieved. 129 The riboswitches that respond to NAD + have also been reported, which have the potential to be utilized for dynamic regulation of intracellular levels of NAD(P)H, ATP, and SAM. 184,185…”

Section: Genetic Circuits For Sensing and Dynamic Regulation Of Intra...mentioning

confidence: 99%

See 2 more Smart Citations

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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Methylated natural products are widely spread in nature. S-Adenosyl-L-methionine (SAM) is the secondary abundant cofactor and the primary methyl donor, which confer natural products with structural and functional diversification. The increasing demand for SAM-dependent natural products (SdNPs) has motivated the development of microbial cell factories (MCFs) for sustainable and efficient SdNP production. Insufficient and unsustainable SAM availability hinders the improvement of SdNP MCF performance. From the perspective of developing MCF, this review summarized recent understanding of de novo SAM biosynthesis and its regulatory mechanism. SAM is just the methyl mediator but not the original methyl source. Effective and sustainable methyl source supply is critical for efficient SdNP production. We compared and discussed the innate and relatively less explored alternative methyl sources and identified the one involving cheap one-carbon compound as more promising. The SAM biosynthesis is synergistically regulated on multilevels and is tightly connected with ATP and NAD(P)H pools. We also covered the recent advancement of metabolic engineering in improving intracellular SAM availability and SdNP production. Dynamic regulation is a promising strategy to achieve accurate and dynamic fine-tuning of intracellular SAM pool size. Finally, we discussed the design and engineering constraints underlying construction of SAM-responsive genetic circuits and envisioned their future applications in developing SdNP MCFs.

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“…coli. Similar results, stable intracellular ATP level through the fermentation and improved GSH (137.40%) and SAM (82.18%) coproduction, were achieved …”

Section: Genetic Circuits For Sensing and Dynamic Regulation Of Intra...mentioning

confidence: 99%

Section: Cofactor Sam Atp and Nad(p)h Regeneration And Connectionsmentioning

confidence: 99%

Section: Genetic Circuits For Sensing and Dynamic Regulation Of Intra...mentioning

confidence: 99%

See 1 more Smart Citation

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…ATP provides energy for the biosynthesis of a large number of biological compounds, such as amino acids, proteins, and lipids [ 6 ]. Large amounts of ATP are also needed in another important field that is cell-free protein expression, as they are involved in complex reaction cascades [ 1 , 7 , 8 ], such as the production of S-adenosyl-homocysteine (SAH), glutathione (GSH), and S-adenosyl-methionine (SAM) [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…It provides a quicker, less energy-efficient source of ATP, and it does not require the FoF1 ATP synthase driven by proton motive forces (PMF) across the membranes compared with oxidative phosphorylation and photophosphorylation to regenerate ATP [ 5 , 11 ]. At the same time, there are other types of ATP regeneration, such as co-production of glutathione and S-adenosyl-methionine [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

Zhao¹,

Yang²,

Xie³

et al. 2022

Biomolecules

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Adenosine triphosphate (ATP), as a universal energy currency, takes a central role in many biochemical reactions with potential for the synthesis of numerous high-value products. However, the high cost of ATP limits industrial ATP-dependent enzyme-catalyzed reactions. Here, we investigated the effect of cell-surface display of phosphotransferase on ATP regeneration in recombinant Escherichia coli. By N-terminal fusion of the super-folder green fluorescent protein (sfGFP), we successfully displayed the phosphotransferase of Pseudomonas brassicacearum (PAP-Pb) on the surface of E. coli cells. The catalytic activity of sfGFP-PAP-Pb intact cells was 2.12 and 1.47 times higher than that of PAP-Pb intact cells, when the substrate was AMP and ADP, respectively. The conversion of ATP from AMP or ADP were up to 97.5% and 80.1% respectively when catalyzed by the surface-displayed enzyme at 37 °C for only 20 min. The whole-cell catalyst was very stable, and the enzyme activity of the whole cell was maintained above 40% after 40 rounds of recovery. Under this condition, 49.01 mg/mL (96.66 mM) ATP was accumulated for multi-rounds reaction. This ATP regeneration system has the characteristics of low cost, long lifetime, flexible compatibility, and great robustness.

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Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae

Xiao,

Shi,

Huang

et al. 2024

Biotechnology Journal

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S‐Adenosyl‐L‐methionine (SAM) is a substrate for many enzyme‐catalyzed reactions and provides methyl groups in numerous biological methylations, and thus has vast applications in the agriculture and medical field. Saccharomyces cerevisiae has been engineered as a platform with significant potential for producing SAM, but the current production has room for improvement. Thus, a method that consists of a series of metabolic engineering strategies was established in this study. These strategies included enhancing SAM synthesis, increasing ATP supply, down‐regulating SAM metabolism, and down‐regulating competing pathway. After combinatorial metabolic engineering, Bayesian optimization was conducted on the obtained strain C262P6S to optimize the fermentation medium. A final yield of 2972.8 mg·L−1 at 36 h with 29.7% of the L‐Met conversion rate in the shake flask was achieved, which was 26.3 times higher than that of its parent strain and the highest reported production in the shake flask to date. This paper establishes a feasible foundation for the construction of SAM‐producing strains using metabolic engineering strategies and demonstrates the effectiveness of Bayesian optimization in optimizing fermentation medium to enhance the generation of SAM.

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ATP dynamic regeneration strategy for enhancing co-production of glutathione and S-adenosylmethionine in Escherichia coli

Cited by 13 publications

References 14 publications

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Improving Microbial Cell Factory Performance by Engineering SAM Availability

Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae

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