Atorvastatin loaded PLGA microspheres: Preparation, HAp coating, drug release and effect on osteogenic differentiation of ADMSCs

Shokrolahi, Fatemeh; Khodabakhshi, Khosrow; Shokrollahi, Parvin; Badiani, Roya; Moghadam, Zohreh Mansoori

doi:10.1016/j.ijpharm.2019.05.005

Cited by 18 publications

(12 citation statements)

References 43 publications

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“…The HAp nanoparticles were examined to determine calcium to phosphorus (Ca/P) ratio. EDX (Figure 1E) was used to carry out the investigation, and the results indicated that the Ca/P ratio was in the range of 1.6–1.8 for the synthesized HAp, which is similar to that found in natural human bones 32 . The (Zn + Ca)/(P) ratio of Zn‐HAp was also examined using EDX analysis, and it was found to be 1.72, which is in good agreement with the Ca/P ratio of natural HAp.…”

Section: Resultssupporting

confidence: 61%

Engineering antibacterial shrinkage‐free trinary PLGA‐based GBR membrane for bone regeneration

Doost,

Shokrolahi,

Shokrollahi

et al. 2024

Polymers for Advanced Techs

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The purpose of this study was preparation of a multilayer electrospun poly (lactic‐co‐glycolic acid) (PLGA)‐based guided bone regeneration (GBR) membrane with controlled shrinkage behavior and alveolar bone regeneration property. First, PLGA copolymer, and zinc‐doped hydroxyapatite (Zn‐HAp) particles were prepared and characterized using fourier transform infrared spectroscopy, differential scanning calorimetry (DSC), proton nuclear magnetic resonance, and X‐ray diffraction analysis. Since the electrospun PLGA scaffolds showed about 80% shrinkage at physiological conditions (phosphate‐buffered saline, 37°C), the effect of factors such as fiber‐alignment, and additives including natural polymers such as gelatin and chitosan, and Zn‐HAp particles; as well as solution preparation method was investigated on the shrinkage ratio. The results showed that it is possible to eliminate the shrinkage of the scaffold in the physiological environment through appropriate design of a tri‐layer PLGA‐CHI/PLGA‐Gel/PLGA‐Gel‐ZnHAp membrane. The designed tri‐layer membrane demonstrated a bubble point smaller than 7 μm, and improved mechanical properties compared with the individual sub‐layer scaffolds. Additionally, it exhibited enhanced antibacterial activity when compared with a similar three‐layer membrane in which HAp was used instead of Zn‐HAp. This observation suggests a synergistic antibacterial effect resulting from the presence of both zinc ions in Zn‐HAp and chitosan . Osteogenic differentiation of adipose‐derived mesenchymal stem cells cultured on the optimal multilayer composite scaffold, was investigated using alkaline phosphatase and Alizarin red staining assays, and the results suggested that the scaffold could support osteogenic differentiation of the stem cells. The designed membrane was implanted in critical size (1 cm) mandibular defects in dogs, and bone regeneration was monitored by computed tomography. Defects treated with the GBR membrane, and the control group showed 69.31% and 44.63%, newly mineralized tissue, respectively, after 8 weeks post implantation. Based on our results, the engineered 3‐layer scaffold is a promising candidate as a GBR membrane for periodontal applications.

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Section: Resultssupporting

confidence: 61%

Engineering antibacterial shrinkage‐free trinary PLGA‐based GBR membrane for bone regeneration

Doost,

Shokrolahi,

Shokrollahi

et al. 2024

Polymers for Advanced Techs

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“…Furthermore, PLGA can improve the bone integration in diabetic environment by inhibiting advanced glycation end products (AGEs) ( Hu et al, 2018 ). In addition, as a drug carrier, PLGA materials can carry most kinds of drugs and control the release rate of drugs ( Shokrolahi et al, 2019 ). Kwon et al (2018) prepared PLGA coating on the 3D-printed titanium alloy implant and loaded it with ethyl 2,5-dihydroxybenzoate (E-2,5-DHB) (a drug that promotes bone formation and inhibits bone resorption).…”

Section: Functionalization Of the Implant Surfacementioning

confidence: 99%

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

Sheng

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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With the development of three-dimensional (3D) printed technology, 3D printed alloy implants, especially titanium alloy, play a critical role in biomedical fields such as orthopedics and dentistry. However, untreated titanium alloy implants always possess a bioinert surface that prevents the interface osseointegration, which is necessary to perform surface modification to enhance its biological functions. In this article, we discuss the principles and processes of chemical, physical, and biological surface modification technologies on 3D printed titanium alloy implants in detail. Furthermore, the challenges on antibacterial, osteogenesis, and mechanical properties of 3D-printed titanium alloy implants by surface modification are summarized. Future research studies, including the combination of multiple modification technologies or the coordination of the structure and composition of the composite coating are also present. This review provides leading-edge functionalization strategies of the 3D printed titanium alloy implants.

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“…This technique also allows step-by-step incorporation of functional additives, e.g., hydroxyapatite and silver nanoparticles, for introducing both cytocompatibility and antibacterial activity [ 63 ]. Shokrolahi et al reported that HA particles might contribute to the osteogenic differentiation of stem cells as well as load and improve the release profile of active agents, playing a crucial role in regenerative medicine [ 64 ].…”

Section: Fabrication and Modification Of Polymeric-based Microparticl...mentioning

confidence: 99%

Biodegradable Microparticles for Regenerative Medicine: A State of the Art and Trends to Clinical Application

et al. 2022

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Tissue engineering and cell therapy are very attractive in terms of potential applications but remain quite challenging regarding the clinical aspects. Amongst the different strategies proposed to facilitate their implementation in clinical practices, biodegradable microparticles have shown promising outcomes with several advantages and potentialities. This critical review aims to establish a survey of the most relevant materials and processing techniques to prepare these micro vehicles. Special attention will be paid to their main potential applications, considering the regulatory constraints and the relative easiness to implement their production at an industrial level to better evaluate their application in clinical practices.

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Atorvastatin loaded PLGA microspheres: Preparation, HAp coating, drug release and effect on osteogenic differentiation of ADMSCs

Cited by 18 publications

References 43 publications

Engineering antibacterial shrinkage‐free trinary PLGA‐based GBR membrane for bone regeneration

Engineering antibacterial shrinkage‐free trinary PLGA‐based GBR membrane for bone regeneration

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

Biodegradable Microparticles for Regenerative Medicine: A State of the Art and Trends to Clinical Application

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