Atomically Dispersed Cu Nanozyme with Intensive Ascorbate Peroxidase Mimic Activity Capable of Alleviating ROS‐Mediated Oxidation Damage

Abstract

Ascorbate peroxidase (APX) as a crucial antioxidant enzyme has drawn attentions for its utilization in preventing cellsfrom oxidative stress responses by efficiently scavenging H 2 O 2 in plants. For eliminating the specific inactivation of natural APXs and regulating the catalytic activity, single-atom nanozymes are considered as promising classes of alternatives with similar active sites and maximal atomic utilization efficiency to natural APXs. Herein, graphitic carbon nitride (g-C 3 N 4 ) anchored with isolated single copper atoms (Cu SAs/CN) is designed as an efficient nanozyme with intrinsic APX mimetic behavior. The engineered Cu SAs/CN exhibits comparable specific activity and kinetics to the natural APXs. Based on the density functional theory (DFT), Cu-N 4 moieties in the active center of Cu SAs/CN are determined to exert such favorable APX catalytic performance, in which the electron transfer between Cu and coordinated N atoms facilitates the activation and cleavage of the adsorbed H 2 O 2 molecules and results in fast kinetics. The constructed Cu SAs/CN nanozyme with superior APX-like performance and high biocompatibility can be applied for effectively protecting the H 2 O 2 -treated cells against oxidative injury in vitro. These findings report the single-atom nanozymes as a successful paradigm for guiding nanozymes to implement APX mimetic performance for reactive oxygen species-related biotherapeutic.

Engineering Antioxidative Cascade Metal-Phenolic Nanozymes for Alleviating Oxidative Stress during Extracorporeal Blood Purification

“…The elemental composition of the Cu-TA NMs is presented in the insertion table in Figure S8. The content of Cu (3.07 at %) is much higher than that of the other reported Cu nanozymes (Cu SAs/CN, Cu-TCPP, or Cu-TA), and the high content of Cu active centers can facilitate the formation of M-TA NMs. The amounts of copper element look different from the SEM and TEM images, due to the different conditions and methods.…”

Single-Atom Nanozymes: Fabrication, Characterization, Surface Modification and Applications of ROS Scavenging and Antibacterial

“…In addition to the ability of SAzyme to simulate CAT, SOD, and GPx to alleviate cellular oxidative damage, Chen et al [ 106 ] used electrochemical deposition to deposit Cu on g-C 3 N 4 and obtained a kind of SAzyme in which 1 Cu atom was coordinated with four N atoms (denoted as Cu–SAs/CN). Cu–SAs/CN possessed ascorbate peroxidase (APX)-like activity, which can decompose H 2 O 2 in the presence of AsA, thereby alleviating cellular oxidative damage.…”

Lighting Up Agricultural Sustainability in the New Era through Nanozymology: An Overview of Classifications and Their Agricultural Applications

“…It has been well recognized that abiotic stress, caused by drought, salt, cold, and heat stress, results in crop growth reduction and yield losses worldwide . One of the main responses of crops to various abiotic stresses is the production of ROS, including O 2 •– , • OH, O 2 1 , and H 2 O 2 , which are detrimental to biomolecules when their levels exceed the cellular capacity for detoxification . A prospective method in nanoembedded agriculture is use of CAT-like and SOD-like nanozymes because of their unique ROS-regulating ability, which can reduce abiotic stresses and thus ensure crop yield.…”

“…, and H 2 O 2 , which are detrimental to biomolecules when their levels exceed the cellular capacity for detoxification. 92 A prospective method in nanoembedded agriculture is use of CAT-like and SOD-like nanozymes because of their unique ROS-regulating ability, which can reduce abiotic stresses and thus ensure crop yield. For example, Lu et al 81 synthesized Mn 3 O 4 NPs with excellent antioxidant enzyme-mimicking activity and used them for foliar application on cucumber crops for 7 days.…”