Atomic force spectroscopic and SPR kinetic analysis of long circular and short ssDNA molecules interacting with single-stranded DNA-binding protein

Identification and Mechanism of Action of the Global Secondary Metabolism Regulator SaraC in Stereum hirsutum

“…Here, we performed a fusion His 6 -tagged SaraC interaction with methylated DNA using surface plasmon resonance (SPR) spectroscopy. This technology is widely used to detect DNA/DNA, DNA/protein, and small molecule/protein interactions; the interaction between analytes was detected in real-time by recording changes in the refractive index of nano-gold film on the chip surface ( 23 , 24 ). In our experiment, 3 oligonucleotides were used as the analytes, having lengths of approximately 9 to 21 bp and methyladenine or methylcytosine in the middle of the sequence: 6mA, CpG and CHH (Table S1).…”