Atomic Force Microscopy of Bacterial Communities

Núñez, Megan E.; Martin, Michael R.; Chan, Phyllis; Duong, Lin; Sindhurakar, Anil; Spain, Eileen M.

doi:10.1016/s0076-6879(05)97015-8

Cited by 18 publications

(9 citation statements)

References 18 publications

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“…This, in turn, generates sufficient noise during scanning such that image quality is compromised [ 35 , 36 ]. In our study, we used air-dried samples since AFM imaging of dried microbial preparations is well established [ 33 , 37 ]. The advantages include easy sample preparation and high-resolution imaging of microbial cell surfaces [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of photodynamic therapy on Enterococcus faecalis biofilms

et al. 2015

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Microbial biofilms are involved in almost all infectious pathologies of the oral cavity. This has led to the search for novel therapies specifically aimed at biofilm elimination. In this study, we used atomic force microscopy (AFM) to visualize injuries and to determine surface roughness, as well as confocal laser scanning microscopy (CLSM) to enumerate live and dead bacterial cells, to determine the effects of photodynamic therapy (PDT) on Enterococcus faecalis biofilms. The AFM images showed that PDT consisting of methylene blue and a 670-nm diode laser (output power 280 mW during 30 s) or toluidine blue and a 628-nm LED light (output power 1000 mW during 30 s) induced severe damage, including cell lysis, to E. faecalis biofilms, with the former also causing an important increase in surface roughness. These observations were confirmed by the increase in dead cells determined using CLSM. Our results highlight the potential of PDT as a promising method to achieve successful oral disinfection.

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Section: Discussionmentioning

confidence: 99%

Effects of photodynamic therapy on Enterococcus faecalis biofilms

et al. 2015

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“…Standard silicon nitride tips were used for these measurements. Many details of the procedures and instrumental parameters for imaging bacterial cells by AFM are provided in our previous publications (1,27,29).…”

Section: Imaging Techniques (I) Fishmentioning

confidence: 99%

“…Although for many years investigations of B. bacteriovorus concentrated on its predatory nature in planktonic environments, significant recent attention has been directed to predation in the more diverse and complex bacterial cell environments characteristic of biofilms (1,21,(26)(27)(28). In previous studies, our collaboration has explored interactions between wild-type Bdellovibrio predators and their prey at interfaces in model biofilm communities.…”

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confidence: 99%

Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates

Ferguson

Núñez

Kim

et al. 2014

Appl Environ Microbiol

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Bdellovibrio bacteriovorus is a Gram-negative predator of other Gram-negative bacteria. Interestingly, Bdellovibrio bacteriovorus 109J cells grown in coculture with Escherichia coli ML-35 prey develop into a spatially organized two-dimensional film when located on a nutrient-rich surface. From deposition of 10 l of a routine cleared coculture of B. bacteriovorus and E. coli cells, the cells multiply into a macroscopic community and segregate into an inner, yellow circular region and an outer, off-white region. Fluorescence in situ hybridization and atomic force microscopy measurements confirm that the mature film is spatially organized into two morphologically distinct Bdellovibrio populations, with primarily small, vibroid cells in the center and a complex mixture of pleomorphic cells in the outer radii. The interior region cell population exhibits the hunting phenotype while the outer region cell subpopulation does not. Crowding and high nutrient availability with limited prey appear to favor diversification of the B. bacteriovorus population into two distinct, thriving subpopulations and may be beneficial to the persistence of B. bacteriovorus in biofilms.

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“…Visualization of the biofilms was done by atomic force microscopy using the SMM-2000 microscope (Proton-MIET Closed JOINT Stock Company, Russia), in contact mode in air environment [13] [18].…”

Section: Methodsmentioning

confidence: 99%

Batumin—A Selective Inhibitor of Staphylococci—Reduces Biofilm Formation in Methicillin Resistant <i>Staphylococcus aureus</i>

Churkina¹,

Vaneechoutte²,

Киприанова³

et al. 2015

OJMM

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The antibiotic batumin, produced by Pseudomonas batumici, has been shown to be highly active against 123 type and reference strains and clinical isolates of 30 Staphylococcus species (including MRSA and small colony variants-(SSCVs) of S. aureus, S. epidermidis and S. haemolyticus). Batumin activity against these bacteria did not depend on the species, origin or resistance to other antibiotics and its MIC was 0.0625 -0.5 µg/ml. Batumin influence on biofilm formation was studied in clinical isolates of S. aureus, S. epidermidis and S. intermedius. Addition of batumin at a concentration of half of the MIC in the broth, i.e. 0.125 µg/ml, decreased the biofilm of 16 out of 20 S. aureus strains to varying degrees. Batumin was more effective against Staphylococcus strains with strong biofilm formation. Using atomic-force microscopy, it could be shown that batumin reduced the number of S. aureus ATCC 25923 adherent cells more than fourfold. The adherent cells of staphylococci were visualized as monolayers of separate islets. A detailed study of the surface of bacterial cells treated with batumin allowed to establish significant reduction of their roughness values. Observed values were typical for planktonic S. aureus cells. The obtained data explain one of the mechanisms of the antimicrobial activity of batumin, which is based оn preventing the formation of S. aureus biofilm. As such, batumin could be considered as an agent offering opportunities

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Atomic Force Microscopy of Bacterial Communities

Cited by 18 publications

References 18 publications

Effects of photodynamic therapy on Enterococcus faecalis biofilms

Effects of photodynamic therapy on Enterococcus faecalis biofilms

Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates

Batumin—A Selective Inhibitor of Staphylococci—Reduces Biofilm Formation in Methicillin Resistant <i>Staphylococcus aureus</i>

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Atomic Force Microscopy of Bacterial Communities

Cited by 18 publications

References 18 publications

Effects of photodynamic therapy on Enterococcus faecalis biofilms

Effects of photodynamic therapy on Enterococcus faecalis biofilms

Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates

Batumin—A Selective Inhibitor of Staphylococci—Reduces Biofilm Formation in Methicillin Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

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Batumin—A Selective Inhibitor of Staphylococci—Reduces Biofilm Formation in Methicillin Resistant <i>Staphylococcus aureus</i>