Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates

Ferguson, Megan A.; Núñez, Megan E.; Kim, Hyeong-Jin; Goffredi, Shana K.; Shamskhou, Elya; Faudree, Leanna; Chang, En-Chih; Landry, Rebecca M.; Ma, Andrew; Choi, Da-Eun; Thomas, Nicholas; Schmitt, Jaclyn L.; Spain, Eileen M.

doi:10.1128/aem.02423-14

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…As such, the addition of prey or lowering of nutrient accessibility results in phenotypic changes and detachment of the biofilm (Medina and Kadouri, 2009). A similar phenomenon was described by Ferguson et al (2014), with B. bacteriovorus forming spatially organized communities of differentiated bacteria, with a central core of predatory active bacteria, and an outer morphologically heterogeneous community of HI cells. The presence of nutrient and lack of access to prey favored the diversification, and it was speculated that this phenotypic change resembling a biofilm may benefit BALOs persistence in the environment.…”

Section: Balos Self-formation Of Biofilmssupporting

confidence: 53%

Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes

et al. 2020

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Bdellovibrio and like organisms (BALOs) are obligate predatory bacteria that selectively prey on a broad range of Gram-negative bacteria, including multidrug-resistant human pathogens. Due to their unique lifestyle, they have been long recognized as a potential therapeutic and biocontrol agent. Research on BALOs has rapidly grown over the recent decade, resulting in many publications concerning molecular details of bacterial predation as well as applications thereof in medicine and biotechnology. This review summarizes the current knowledge on biotechnological potential of obligate predatory bacteria and their secreted enzymes.

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Section: Balos Self-formation Of Biofilmssupporting

confidence: 53%

Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes

et al. 2020

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“…It is also worthy to mention that the hit locus and its nearby pilus genes (Bd0108~Bd0119) were downregulated by approximately 2~3fold (Table S3, worksheet 4). Given the importance of this locus for predation [30,31] and its high expression level in the attack phase compared to the intraperiplasmic phase [9,32], this might partially explain why B. bacteriovorus predation is less efficient in rich media as previously found by other groups [33,34]. In Rotem et al, it was found that incubating planktonic AP predatory cells with ghost prey cells together with either PYE medium or prey extract led to the silencing of the hit locus (Bd0108) and that was attributed mainly to the presence of the ghost prey cells [10].…”

Section: Discussionmentioning

confidence: 77%

Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth

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et al. 2017

Microb Ecol

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Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding for critical enzymes involved in amino acid biosynthesis. This study, however, found that planktonic attack-phase predators are not just dependent upon prey for nutrients, but rather, they respond to nutrients in the surrounding medium and, subsequently, synthesize and secrete proteases in a nutrient-dependent manner. The major secreted proteases were identified through mass spectrometry analyses. Subsequent RT-qPCR analyses found that the nutrient-induced proteases are similar to those expressed within the prey periplasm during the late intraperiplasmic growth phase. Furthermore, RNA sequencing found that incubating the planktonic attack-phase cells in a nutritious environment for a short period of time (4 h) changes its gene expression pattern to a status that is akin to the late intraperiplasmic phase, with more than 94% of the genes previously identified as being late intraperiplasmic-specific also being induced by nutrient broth in this study. This strong correlation between the gene expression patterns hints that the availability of hydrolyzed prey cell components to the predator is likely the stimulus controlling the expression of late intraperiplasmic B. bacteriovorus genes during predation.

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“…In short, a culture of B. bacteriovorus 109J exponentially growing on P. fluorescens LP6a was harvested, centrifuged at 1000 g for 10 min, filtered through a 0.45 μm pore-size Millex filter and re-suspended in HEPES metals (HM) buffer (Ferguson et al, 2014). About 0.3 ml of this suspension was placed in a small chamber formed by a U-shaped capillary placed on a microscope slide and closed with a glass coverslip in order to prevent the formation of air bubbles within the chamber.…”

Section: Dispersal Experimentsmentioning

confidence: 99%

Catch me if you can: dispersal and foraging ofBdellovibrio bacteriovorus109J along mycelia

et al. 2016

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To cope with heterogeneous environments and resource distributions, filamentous fungi have evolved a spatially extensive growth enabling their hyphae to penetrate air-water interfaces and pass through air-filled pores. Such mycelia are also known to act as dispersal networks for the mobilisation of bacteria ('fungal highways') and connection of microbial microhabitats. Hitherto, however, nothing is known about the effect of mycelia-based dispersal on interactions between bacterial predators and their prey and concomitant effects on biomass formation. We here hypothesise that mycelia enable the contact between predators and their prey and shape a prey's population. We investigated the impact of predation by Bdellovibrio bacteriovorus 109J on the growth of its potential prey Pseudomonas fluorescens LP6a in the presence of mycelia. Our data give evidence that hyphae increase the accessibility of the prey to B. bacteriovorus 109J and, hence, allow for efficient foraging and shaping of prey populations not seen in the absence of mycelia. To test our hypothesis tailored microbial landscapes were used for better reduction of emerging properties in complex systems. Our data suggest that mycelia have substantial influence on prey-predator relationship and hereby may promote the structure of prey and predator populations and, hence, may be a determinant for biomass formation in heterogeneous environments.

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Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates

Cited by 7 publications

References 50 publications

Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes

Biotechnological Potential of Bdellovibrio and Like Organisms and Their Secreted Enzymes

Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth

Catch me if you can: dispersal and foraging ofBdellovibrio bacteriovorus109J along mycelia

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