Atomic force microscopy in a liquid and in situ ellipsometry as complementary techniques for the study of protein adsorption

Almeida, Arlete Tavares; Gliemann, Hartmut; Schimmel, Thomas; Petri, Denise Freitas Siqueira

doi:10.1007/b97082

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…Various techniques have been developed to investigate protein adsorption, such as surface plasmon resonance (SPR) [5] , atomic force microscopy (AFM) [6] , dynamic contact-angle (DCA) and quartz crystal microbalance (QCM) [7] . In particularly, quartz crystal microbalance with dissipation monitoring (QCM-D) [8] based upon the piezoelectric effect has been regarded as a powerful tool for studying protein adsorption behaviors at the solution-surface interface with sensitivity in the ng/cm 2 range, and can provide the information about the formation, structure and properties of adsorbed protein layers without the necessity of chemical labeling, since QCM-D allows time-resolved simultaneous measurements of changes in frequency (f ) and energy dissipation (D) related to the mass loading and the viscoelastic properties of the adsorbed layers, respectively.…”

Section: Introductionmentioning

confidence: 99%

Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

Zeng

Zhang

Liang

et al. 2009

Sci. China Ser. E-Technol. Sci.

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Chitosan/poly(ε-caprolactone) (PCL) blend films in different mass ratios were prepared using the chitosan/PCL mixture solutions in 80 vol.-% acetic acid by spin coating. Their surface micromorphologies were assessed by atomic force microscopy (AFM). It was found that the micromorphology of chitosan/PCL blend films was in large extent related to the mass ratio of chitosan. 25 wt% chitosan/PCL blend film presented microphase separation. The protein adsorption of bovine serum albumin (BSA) onto chitosan/PCL blend films was investigated by using quartz crystal microbalance with dissipation monitoring (QCM-D) in real time. The results suggested that the amount of adsorbed BSA on the chitosan/PCL blend films decreased with the addition of chitosan, but the structure and viscoelastic properties of the adsorbed BSA layers were greatly affected by the surface micromorphology of chitosan/PCL blend films. BSA absorbed on the 25 wt% chitosan/PCL blend film with microphase separation showed larger adsorption reversibility, and preferred to form a loose, dissipative layer in comparison with those on other chitosan/PCL blend films without microphase separation.protein adsorption, chitosan, poly(ε -caprolactone), quartz crystal microbalance with dissipation monitoring (QCM-D), surface micromorphology

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Section: Introductionmentioning

confidence: 99%

Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

Zeng

Zhang

Liang

et al. 2009

Sci. China Ser. E-Technol. Sci.

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“…[5][6][7] The advance of instrumental techniques has allowed one to go deeper into the study of adsorption behavior of protein in liquid. Combined in situ measurements provide reliable information about the thickness, adsorbed amount, and adsorption kinetics with the corresponding three-dimensional structural arrangement, 8 without any artifact due to drying process. [9][10][11] Creatine phosphokinase (CPK) has been largely used in clinical assays for the diagnosis of myocardial infarction and muscular disease.…”

Section: Introductionmentioning

confidence: 99%

“…Not only the adsorbed amount of protein or enzyme but also the biomolecule orientation upon adsorption affects the activity and stability of the immobilized entities. − The advance of instrumental techniques has allowed one to go deeper into the study of adsorption behavior of protein in liquid. Combined in situ measurements provide reliable information about the thickness, adsorbed amount, and adsorption kinetics with the corresponding three-dimensional structural arrangement, without any artifact due to drying process. − …”

Section: Introductionmentioning

confidence: 99%

Effect of pH on the Adsorption and Activity of Creatine Phosphokinase

et al. 2006

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The combination of in situ ellipsometry with atomic force microscopy in the liquid for the study of adsorption of creatine phosphokinase (CPK) onto silicon wafers was shown for the first time. The thickness, adsorbed amount, and topographic information of the adsorbed CPK layers were obtained under different pH conditions. The thickness values of adsorbed CPK layer determined by both techniques were in excellent agreement. At pH 4, CPK monomers present in solution adsorb, forming a very thin (approximately 0.8 nm) layer, indicating CPK unfolding. Upon increasing the pH to 6.8, the adsorbed layer is composed of a mixture of CPK dimers (native structure) and intermediates, increasing the film thickness (approximately 2.4 nm). At pH 9, CPK dimers form monolayers with the highest thickness (approximately 4.0 nm). The nature of interactions between CPK and Si wafers associated with the hydration force seems to control the degree of CPK unfolding upon adsorbing. The enzymatic activity of free CPK and of adsorbed CPK at pH 4, pH 6.8, and pH 9 was measured as a function of pH. In comparison to free CPK in solution, adsorbed CPK presented a shift of the optimal pH from 6.8 toward alkaline pH.

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Biomimetic coatings for biomaterial surfaces

Chánová

Rypáček

2013

Biomimetic Biomaterials

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Atomic force microscopy in a liquid and in situ ellipsometry as complementary techniques for the study of protein adsorption

Cited by 3 publications

References 3 publications

Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

Effect of pH on the Adsorption and Activity of Creatine Phosphokinase

Biomimetic coatings for biomaterial surfaces

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