Protein adsorption behaviors on chitosan/poly(ɛ-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring (QCM-D)

Abstract

Chitosan/poly(ε-caprolactone) (PCL) blend films in different mass ratios were prepared using the chitosan/PCL mixture solutions in 80 vol.-% acetic acid by spin coating. Their surface micromorphologies were assessed by atomic force microscopy (AFM). It was found that the micromorphology of chitosan/PCL blend films was in large extent related to the mass ratio of chitosan. 25 wt% chitosan/PCL blend film presented microphase separation. The protein adsorption of bovine serum albumin (BSA) onto chitosan/PCL blend films was investigated by using quartz crystal microbalance with dissipation monitoring (QCM-D) in real time. The results suggested that the amount of adsorbed BSA on the chitosan/PCL blend films decreased with the addition of chitosan, but the structure and viscoelastic properties of the adsorbed BSA layers were greatly affected by the surface micromorphology of chitosan/PCL blend films. BSA absorbed on the 25 wt% chitosan/PCL blend film with microphase separation showed larger adsorption reversibility, and preferred to form a loose, dissipative layer in comparison with those on other chitosan/PCL blend films without microphase separation.protein adsorption, chitosan, poly(ε -caprolactone), quartz crystal microbalance with dissipation monitoring (QCM-D), surface micromorphology

Monitoring Cell Adhesion on Polycaprolactone–Chitosan Films with Varying Blend Ratios by Quartz Crystal Microbalance with Dissipation

“… 42 The induced protein unfolding can also lead to irreversible protein adsorption. 46 The pure PCL film has an IEP of pH 3.2 ( Figure S2 ) below the IEP of fibrinogen at pH 5.8. 47 Thus, fibrinogen adsorbed to pure PCL films under electrostatically repulsive conditions, while it is known that protein adsorption also can take place under these conditions, in principle.…”

“…Proteins bind more strongly onto hydrophobic surfaces compared to hydrophilic ones because the contact of proteins with hydrophobic surfaces leads to changes in the protein conformation and to the release of water due to reorientation of hydrophobic amino acid residues at the interface . The induced protein unfolding can also lead to irreversible protein adsorption . The pure PCL film has an IEP of pH 3.2 (Figure S2) below the IEP of fibrinogen at pH 5.8 .…”

“…Besides a small positive net charge of the surface (IEP of chitosan at pH 6.8; see Figure S2), the chitosan film is hydrophilic and has a high water retention capability, which leads to a water hydration layer at the polymer–solution interface . Proteins keep their native structure, and weak, mostly reversible protein adsorption occurs. , As a consequence, fibrinogen does not adsorb significantly on pure chitosan films due to surface hydrophilicity, while the electrostatics are screened by the high ion content in the solution. So far, we have not discussed the influence of roughness on protein adsorption.…”

Biosorption of cadmium(II) from aqueous solution by chitosan encapsulated Zygosaccharomyces rouxii

“…On the one hand, immobilized CZ could protect Z. rouxii from exposing directly to Cd(II) solution all the time and ease the cell membrane damage caused by Cd(II). On the other hand, chitosan in CZ had the capability to adsorption organics such as nucleic acids and protein . Cell membrane of Z. rouxii was less damaged at low pH (1.0–2.0) because it adsorbed less Cd(II) (Figure ), so the leaching amount of Z. rouxii was relative low.…”

Preparation, Properties and Applications of Chitosan-Based Biocomposites/Blend Materials: A Review

“…The hydrophobicity as well as the lack of functional groups of PCL is reduced by incorporation of Cs matrix [235]. This strategy suggests that the hydrophobicity of PCL makes it possible to produce a blended material with good bio-compatibility [236], full bio-degradability [237,238], adequate mechanical strength [239,240] and one that is available for bio-functions [241]. The PCL/Cs blend was prepared by cast solution technique [242][243][244], electro-spinning [241] and semi-interpenetrating polymer networks [245].…”