Atomic force microscopy characterization of an electrochemical DNA-biosensor

Chiorcea, Ana-Maria; Oliveira-Brett, Ana Maria

doi:10.1016/j.bioelechem.2003.09.029

Cited by 44 publications

(23 citation statements)

References 8 publications

(12 reference statements)

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“…Different immobilization procedures of monolayer and multilayer DNA films on highly oriented pyrolytic graphite (HOPG) electrodes have been characterized by Magnetic AC Mode Atomic Force Microscopy (MAC AFM) [14-17]. HOPG provides a particularly smooth electrode surface, Fig.…”

Section: Dna-electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical DNA Sensors for Detection of DNA Damage

Diculescu

Chiorcea-Paquim

Oliveira-Brett

2005

Sensors

119

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Electrochemical devices have received particular attention due to their rapid detection and great sensitivity for the evaluation of DNA-hazard compounds interaction mechanisms. Several types of bioanalytical method use nucleic acids probes to detect DNA damage. This article reviews current directions and strategies in the development and applications of electrochemical DNA sensors for the detection of DNA damage.

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Section: Dna-electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical DNA Sensors for Detection of DNA Damage

Diculescu

Chiorcea-Paquim

Oliveira-Brett

2005

Sensors

119

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“…20 nm), pointing out to the bending of the probe. As it has been reported for proteins [49 -51], or nucleic acids [52,53] the shape of adsorbed biomolecules is strongly affected by the underlying surface properties. In this case, AFM imaging reveals that adsorbed ss-DNA apparently displays a globular shape, as a result of its covalent adsorption onto the 4-ATP SAM, that can be in part influenced by the tip shape [52].…”

Section: Characterization Of the Modified Electrodes By Atomic Force mentioning

confidence: 90%

4‐Aminothiophenol Self‐Assembled Monolayer for the Development of a DNA Biosensor Aiming the Detection of Cylindrospermopsin Producing Cyanobacteria

2008

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The development of a DNA biosensor for the detection of cylindrospermopsin, based on self-assembled monolayers (SAMs) of 4-aminothiophenol, is investigated. SAMs were characterized by electrochemical reductive desorption. Detection of probe immobilization and hybridization has been achieved by cyclic and square-wave voltammetry (SWV), using methylene blue (MB) as electroactive indicator. The SWV data obtained in phosphate buffer, with and without NaCl, after MB accumulation, revealed an increase of the redox indicator current peaks after the hybridization step. This behavior is consistent with MB intercalation into DNA, for high ionic strength media and attributed to electrostatic interactions in the absence of salt. Evidence for surface modification is also provided by atomic force microscopy and ellipsometry.

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“…Extensive studies using atomic force microscopy (AFM) have been undertaken in order to clarify the adsorption behavior of DNA at carbon electrode surfaces [34][35][36][37][38]. The visualization of soft molecules, like DNA, weakly bound to the conducting surface of electrochemical transducers is better achieved using magnetic AC mode atomic force microscopy (MAC Mode AFM), which enables imaging the surface morphological structure of adsorbed nucleic acid and understanding the mechanism of adsorption and the nature of DNA-electrode surface interactions [34].…”

Section: Afm Characterisation Of the Dsdna-electrochemical Biosensor mentioning

confidence: 99%

DNA-Electrochemical Biosensors: AFM Surface Characterisation and Application to Detection of In Situ Oxidative Damage to DNA

Oliveira¹,

Oliveira‐Brett

2010

CCHTS

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In recent years increased attention has been focused on the ways in which drugs interact with DNA, with the goal of understanding the toxic as well as chemotherapeutic effects of many molecules. The development of fast and accurate methods of DNA damage detection is important, especially caused by anticancer drugs or hazard compounds. The DNA-electrochemical biosensor is a very good model for evaluation of nucleic acid damage, and electrochemical detection a particularly sensitive and selective method for the investigation of specific interactions. The electrochemical sensor for detecting DNA damage consists of a glassy carbon electrode with DNA immobilized on its surface. The starting materials or the redox reaction products can be pre-concentrated on the dsDNA-biosensor surface, enabling electrochemical probing of the presence of short-lived radical intermediates and of their damage to dsDNA. AFM images were used to characterize different procedures for immobilization of nanoscale DNA surface films on carbon electrodes before and after interaction with hazard compounds. The electrochemical transduction is dynamic in that the electrode is itself a tuneable charged reagent as well as a detector of all surface phenomena, which greatly enlarges the electrochemical biosensing capabilities.

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Atomic force microscopy characterization of an electrochemical DNA-biosensor

Cited by 44 publications

References 8 publications

Electrochemical DNA Sensors for Detection of DNA Damage

Electrochemical DNA Sensors for Detection of DNA Damage

4‐Aminothiophenol Self‐Assembled Monolayer for the Development of a DNA Biosensor Aiming the Detection of Cylindrospermopsin Producing Cyanobacteria

DNA-Electrochemical Biosensors: AFM Surface Characterisation and Application to Detection of In Situ Oxidative Damage to DNA

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