Atomic force microscopy as a tool of inspection of viral infection

Dubrovin, Evgeniy V.; Drygin, Yuri-F.; Novikov, V. K.; Яминский, И.В.

doi:10.1016/j.nano.2007.01.005

Cited by 18 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AFM, as no invasive technique, is a powerful device for virological studies, mostly for investigation of virion structures and interaction with other molecules [24][25][26][27]. The virological studies using this method are being in progress in our laboratory.…”

Section: Characterization Of the Electrochemical Immunosensormentioning

confidence: 99%

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

et al. 2011

View full text Add to dashboard Cite

This paper concerns the development of an immunosensor for detection of Plum pox virus in plant extracts. The sensor was based on gold electrodes modified with: 1,6-hexanedithiol, gold nanoparticles, anti-PPV IgG polyclonal antibody and BSA. It was used for determination of the virus in extracts from plum (Prunus domestica) and tobacco (Nicotiana benthamiana) leaves. The proposed electrochemical impedance spectroscopy (EIS) immunosensor displayed a very good detection limit of 10 pg/mL and a wide dynamic range from 10 pg PPV/ mL to 200 pg/mL. The presence of extracts from plant materials has no influence on the immunosensors response. The immunosensor was capable of discriminating between samples from healthy plants and samples containing 0.01 % of extract from infected plant material. The conditions for immunosensor regeneration are also presented.

show abstract

Section: Characterization Of the Electrochemical Immunosensormentioning

confidence: 99%

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Both options are seriously considered, however the former has the advantage of simplicity. Since viral capsids have well-defined shapes, it is relatively easy to detect and classify them by AFM in very small amounts of crude samples, including the surface of fixed cells [58,144,145]. The VIPER database of icosahedral capsids (http://viperdb.scripps.edu) provides the X-ray crystallography and cryoEM data on several hundreds of structures, which should be useful to guide interpretation of AFM data [146].…”

Section: Afm Of Protein Complexes In Nanomedicine and Nanobio Techmentioning

confidence: 99%

AFM of biological complexes: What can we learn?

Gaczyńska

Osmulski

2008

Current Opinion in Colloid & Interface Science

View full text Add to dashboard Cite

The term “biological complexes” broadly encompasses particles as diverse as multisubunit enzymes, viral capsids, transport cages, molecular nets, ribosomes, nucleosomes, biological membrane components and amyloids. The complexes represent a broad range of stability and composition. Atomic force microscopy offers a wealth of structural and functional data about such assemblies. For this review, we choose to comment on the significance of AFM to study various aspects of biology of selected nonmembrane protein assemblies. Such particles are large enough to reveal many structural details under the AFM probe. Importantly, the specific advantages of the method allow for gathering dynamic information about their formation, stability or allosteric structural changes critical for their function. Some of them have already found their way to nanomedical or nanotechnological applications. Here we present examples of studies where the AFM provided pioneering information about the biology of complexes, and examples of studies where the simplicity of the method is used toward the development of potential diagnostic applications.

show abstract

“…AFM is applicable to study chemically induced morphological changes at the nanometre level. AFM has also been used for accurate determination of the size and shape of virus particles, the architecture and mechanical properties of their surfaces including elasticity or brittleness, because viruses are subjected to several internal and external forces (Kuznetsov et al , 2001; Chen, 2007; Dubrovin et al , 2007; Mateu, 2012). We have previously reported the use of AFM for studying the adhesion and mechanical properties of RSV infected HEp-2 cells (Pfendt et al , 2011).…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopic investigation of respiratory syncytial virus infection in HEp‐2 cells

Tiwari

Eroğlu

Boyoglu-Barnum

et al. 2013

Journal of Microscopy

View full text Add to dashboard Cite

Summary Respiratory syncytial virus (RSV) primarily causes bronchiolitis and pneumonia in infants. In spite of intense research, no safe and effective vaccine has been developed yet. For understanding its pathogenesis and development of anti-RSV drugs/therapeutics, it is indispensable to study the RSV–host interaction. Although, there are limited studies using electron microscopy to elucidate the infection process of RSV, to our knowledge, no study has reported the morphological impact of RSV infection using atomic force microscopy. We report the cytoplasmic and nuclear changes in human epidermoid cell line type 2 using atomic force microscopy. Human epidermoid cell line type 2 cells, grown on cover slips, were infected with RSV and fixed after various time periods, processed and observed for morphological changes using atomic force microscopy. RSV infected cells showed loss of membrane integrity, with degeneration in the cellular content and cytoskeleton. Nuclear membrane was disintegrated and nuclear volume was decreased. The chromatin of the RSV infected cells was condensed, progressing towards degeneration via pyknosis and apoptosis. Membrane protrusions of ~150–200 nm diameter were observed on RSV infected cells after 6 h, suggestive of prospective RSV budding sites. To our knowledge, this is the first study of RSV infection process using atomic force microscopy. Such morphological studies could help explore viral infection process aiding the development of anti-RSV therapies.

show abstract

Atomic force microscopy as a tool of inspection of viral infection

Cited by 18 publications

References 10 publications

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

AFM of biological complexes: What can we learn?

Atomic force microscopic investigation of respiratory syncytial virus infection in HEp‐2 cells

Contact Info

Product

Resources

About