Atomic force microscopy and theoretical considerations of surface properties and turgor pressures of bacteria

Immunoimmobilization of Living Salmonella for Fundamental Studies and Biosensor Applications

“…In our experiments, the average modulus of elasticity was calculated to be 0.4±0.2×10 6 Pa for living S. Typhimurium cells in growth medium or in PBS buffer. This is comparable to results reported for P. aeruginosa and E. coli (Ingraham and Marr 1996;Yao et al 2002).…”

Force measurements with the atomic force microscope: Technique, interpretation and applications

“…Application of this model to indentation curves on the bacterium Magnetospirillum gryphiswaldense showed that measured cell response is dominated by the turgor pressure of $10 5 Pa. Similar values of turgor pressure were found for Pseudomonas aeruginosa and Enterococcus hirae bacteria [918]. A complementary approach by Scheffer et al [919] was to obtain the membrane bending stiffness from the shape of the force curve during detachment prior to jump-out using a model of axisymmetric bending of a thick annular plate [920].…”

Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

“…On the experimental front, the elastic properties of a cell wall were quantified by: i) squashing a single cell [37,38]; ii) central point loading membrane clamped between two fixtures [39]; and iii) indenting a capsule by an atomic force microscope (AFM) tip [40][41][42]. Theoretically, the classical theory of large elastic deformation of a membrane has been determined for: i) gas-filled spherical membranes under plane stress [43]; ii) fluid-filled spherical shells under plane stress [37,42]; iii) fluid-filled spherical shells under pure bending [41]; and iv) homogeneous solid spheres [44]. Despite the above mentioned developments, scant attention has been paid to the mechanical modelling of biological cells, with the exception of Sun's biomembrane point-load model [21].…”