Atomic force microscopy analysis shows surface structure changes in carvacrol-treated bacterial cells

Storia, Antonietta La; Ercolini, Danilo; Marinello, Francesco; Pasqua, Rosangela Di; Villani, Francesco; Mauriello, Gianluigi

doi:10.1016/j.resmic.2010.11.006

Cited by 129 publications

(63 citation statements)

References 34 publications

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“…The authors also suggested that CAR possesses an ATPase inhibitory activity (Gill and Holley, 2006). Storia et al (2011) noted that gram-negative bacteria exhibited a greater increase in roughness after exposure to CAR than grampositive bacteria did. The action of this compound may render the components of the outer membrane (proteins and lipids) more exposed to the external surface, causing an increase in the cell surface roughness, as observed in our work.…”

Section: Discussionmentioning

confidence: 99%

Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth

Sousa

Torres

Azerêdo

et al. 2012

International Journal of Food Microbiology

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Section: Discussionmentioning

confidence: 99%

Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth

Sousa

Torres

Azerêdo

et al. 2012

International Journal of Food Microbiology

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“…[27] The nanoemulsion treated, and untreated cells were washed twice with sterile distilled water to eliminate the salt interference during the analysis. This was performed in contact mode using Nanosurf Easy Scan 2, Switzerland.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes

Chandrasekaran¹,

Sugumar²,

Mukherjee³

2015

Asian J Pharm

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A transparent oil-in-water nanoemulsion system consisting of eucalyptus oil, Tween-20 as organic phase and water as an aqueous phase was developed using a low energy emulsification method. Physicochemical properties such as droplet size, optical transparency, and long-term stability was studied. The stable eucalyptus oil nanoemulsion (1:2) having mean droplet size in the range of 50-100 nm with a polydispersity index <0.2. The optimized nanoemulsion formulation exhibited significantly higher antibacterial activity by the well diffusion method against Listeria monocytogenes. Further, alteration in the membrane integrity was assessed, and it is found higher for nanoemulsion treated cells than control cells. Atomic force microscopic observations showed distorted morphology of treated bacterial cells. These results propose the possible use of eucalyptus oil nanoemulsion in the food industries. H o w t o c i t e t h i s a r t i c l e : S u g u m a r S , M u k h e r j e e A , Chandrasekaran N. Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes. Asian J Pharm 2015;9:23-8.

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“…At sublethal concentrations, carvacrol changes the fatty acid compositions of the cytoplasmatic membrane in different microorganisms -E. coli O157:H7, S. enterica serovar Typhimurium, Pseudomonas fluorescens, Brochothrix thermosphacta and S. aureus -increasing the unsaturated fatty acid content [80]. Recently, the atomic force microscopy analysis confirmed the cell envelope as major target of carvacrol and enhanced the knowledge on the surface structure changes: the cell size, length and diameter decreased after carvacrol contact [81]. This kind of interaction leads to the formation of membrane channels for ions, cellular material, ATP and nucleic acid to leave the cytoplasm.…”

Section: The Action Mechanismmentioning

confidence: 99%

Antimicrobial Activity of Carvacrol: Current Progress and Future Prospectives

Nostro

Papalia²

2012

PRI

189

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During the last few years the scientific community has shown a considerable interest in the study of plant materials as sources of new compounds to be processed into antimicrobial agents. In this context, carvacrol, a monoterpenic phenol, has emerged for its wide spectrum activity extended to food spoilage or pathogenic fungi, yeast and bacteria as well as human, animal and plant pathogenic microorganisms including drug-resistant and biofilm forming microorganisms. The antibacterial activity of carvacrol has been attributed to its considerable effects on the structural and functional properties of cytoplasmatic membrane. The data reported in this review provide an overview of the published literature regarding the antimicrobial properties of carvacrol and the recent patents claimed in order to highlight its future applications as a new antimicrobial agent. These could concern either the natural preservation in the cosmetic and food industries or an alternative which supports the conventional antimicrobial protocols. Interestingly, carvacrol alone or associated with one or more synergistic products could be incorporated in different formulations for biomedical and food packaging applications. However, more detailed safety investigations and in vivo studies should be carried out so that this molecule could be used in the future.

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Atomic force microscopy analysis shows surface structure changes in carvacrol-treated bacterial cells

Cited by 129 publications

References 34 publications

Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth

Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth

Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes

Antimicrobial Activity of Carvacrol: Current Progress and Future Prospectives

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