“…AFM, which was invented in 1986, expanded the application of scanning tunneling microscopy to nonconductive, soft, and living biological samples [85][86][87][88]. AFM has several capabilities, including imaging topographic details of surfaces from the submolecular level to the cellular level [89], monitoring the dynamic processes of single molecules in physiologically relevant solutions [90], measuring molecular interactions [91], and characterizing the mechanical properties of single molecules or single nanostructures [92].…”