Atomic-absorption spectrometric determination of calcium, magnesium and potassium in leaf samples after decomposition with molten sodium hydroxide

Gimeno-Adelantado, José Vicente; Martínez, Vladimir; García, Adrián; Reig, F. Bosch

doi:10.1016/0039-9140(91)80309-n

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…The LOQ was 2.00 µg L −1 . These particular performance characteristics exceed the values reported in the literature, e.g., for the determination of magnesium using AAS in leaf samples of different origins [26][27][28][29].…”

Section: Calibration and Figures Of Meritcontrasting

confidence: 74%

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

Krüger,

Weng,

Baecker

2023

Molecules

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The Chinese yam (Dioscorea polystachya, DP) is known for the nutritional value of its tuber. Nevertheless, DP also has promising pharmacological properties. Compared with the tuber, the leaves of DP are still very little studied. However, it may be possible to draw conclusions about the plant quality based on the coloration of the leaves. Magnesium, as a component of chlorophyll, seems to play a role. Therefore, the aim of this research work was to develop an atomic absorption spectrometry-based method for the analysis of magnesium (285.2125 nm) in leaf extracts of DP following the graphite furnace sub-technique. The optimization of the pyrolysis and atomization temperatures resulted in 1500 °C and 1800 °C, respectively. The general presence of flavonoids in the extracts was detected and could explain the high pyrolysis temperature due to the potential complexation of magnesium. The elaborated method had linearity in a range of 1–10 µg L−1 (R2 = 0.9975). The limits of detection and quantification amounted to 0.23 µg L−1 and 2.00 µg L−1, respectively. The characteristic mass was 0.027 pg, and the recovery was 96.7–102.0%. Finally, the method was applied to extracts prepared from differently colored leaves of DP. Similar magnesium contents were obtained for extracts made of dried and fresh leaves. It is often assumed that the yellowing of the leaves is associated with reduced magnesium content. However, the results indicated that yellow leaves are not due to lower magnesium levels. This stimulates the future analysis of DP leaves considering other essential minerals such as molybdenum or manganese.

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Section: Calibration and Figures Of Meritcontrasting

confidence: 74%

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

Krüger,

Weng,

Baecker

2023

Molecules

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“…The lysates were transferred into 1.5 mL Eppendorf tubes and centrifuged at 10 000g at 4 °C for 5 min. The supernate was collected and the total protein content and calcium content were measured by the bicinchoninic acid (BCA) method and the atomic absorption method 13 respectively. The results of calcium uptake were expressed in μg mg −1 protein.…”

Section: Calcium Releasing Percentagementioning

confidence: 99%

Fabrication and characterization of the nano-composite of whey protein hydrolysate chelated with calcium

et al. 2015

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The nano-composites of whey protein hydrolysate (WPH) chelated with calcium were fabricated in aqueous solution at 30 °C for 20 min, with the ratio of hydrolysate to calcium 15 : 1 (w/w). UV scanning spectroscopy, fluorescent spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering and atomic force microscopy were applied to characterize the structure of the WPH-calcium chelate. The nano-composites showed the successful incorporation of calcium into the WPH, indicating the interaction between calcium and WPH. The chelation of calcium ions to WPH caused molecular folding and aggregation which led to the formation of a WPH-calcium chelate of nanoparticle size, and the principal sites of calcium-binding corresponded to the carboxyl groups and carbonyl groups of WPH. The WPH-calcium chelate demonstrated excellent stability and absorbability under both acidic and basic conditions, which was beneficial for calcium absorption in the gastrointestinal tract of the human body. Moreover, the calcium absorption of the WPH-calcium chelate on Caco-2 cells was significantly higher than those of calcium gluconate and CaCl₂ in vitro, suggesting the possible increase in calcium bioavailability. The findings suggest that the WPH-calcium chelate has the potential in making dietary supplements for improving bone health of the human body.

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“…The mineral contents of the plants were determined by the procedure of Adelantado et al 63 . Calcium, potassium, magnesium, phosphorus, iron and other elements…”

Section: Determination Of the Mineral Compositionmentioning

confidence: 99%

Evaluation of Phytochemical Constituents and Elemental Profiling of Selected Medicinal Plants in South-West, Nigeria

Usin,

Okon,

Daramola

et al. 2024

Asian J. Biol. Sci

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Background and Objective: Phytochemicals are responsible for the definite physiological effects exerted on the human body by various parts of the plant and it has been reported to play a major role in nutrition and control of diseases. This study aimed to comparatively investigate the phytochemical and elemental components of five medicinal plants commonly used in South-Western, Nigeria. Materials and Methods: Qualitative and quantitative phytochemical screening of all the extracts was done using different standard methods. Calcium, potassium, magnesium, phosphorus, iron and other elements were determined using standard procedures. Results: Preliminary qualitative phytochemical screening indicates the presence of glycosides, saponins, tannins, flavonoids, alkaloids and anthraquinones. The quantitative phytochemical screening revealed that glycosides, saponins, tannins, flavonoids, alkaloids and anthraquinones were significantly (p<0.05) higher in ethanol extract compared to ethyl acetate extract in all the studied medicinal plants, however, the reverse was the case in Urena lobata. Sodium (Na), potassium (K) and calcium (Ca) were significantly (p<0.05) higher in ethyl acetate extract than in ethanol extract in all the studied medicinal plants. While, iron (Fe), lead (Pb), cadmium (Cd) and arsenic (As) were significantly (p<0.05) higher in ethanol extract compared to ethyl acetate extract in all the studied medicinal plants. Conclusion: This study suggests the different range of phytochemicals present in the medicinal plants studied with their potential therapeutic benefits, emphasizing the significance of these plants in traditional and modern medicine. Furthermore, the elemental composition sheds light on the nutritional and essential value of these plants, which could contribute to their holistic healing properties.

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Atomic-absorption spectrometric determination of calcium, magnesium and potassium in leaf samples after decomposition with molten sodium hydroxide

Cited by 12 publications

References 18 publications

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

Development and Application of an Atomic Absorption Spectrometry-Based Method to Quantify Magnesium in Leaves of Dioscorea polystachya

Fabrication and characterization of the nano-composite of whey protein hydrolysate chelated with calcium

Evaluation of Phytochemical Constituents and Elemental Profiling of Selected Medicinal Plants in South-West, Nigeria

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