atoh1.2 and beta3.1 are two new bHLH-encoding genes expressed in selective precursor cells of the zebrafish anterior hindbrain

Adolf, Birgit; Bellipanni, Gianfranco; Huber, Veronica; Bally‐Cuif, Laure

doi:10.1016/j.modgep.2004.06.009

Cited by 36 publications

(31 citation statements)

References 11 publications

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“…To further characterise the nature of the expanded r1 area in morphant embryos, we analysed the expression of markers of cerebellar differentiation. We find that the expanded r1 expresses atoh1a and atoh1b, two granule cell markers (Adolf et al, 2004;Koster and Fraser, 2001) ( Fig. 2G-J; Fig.…”

Section: Transformation Of the Midbrain Into Rhombomere 1 In Otxh Mormentioning

confidence: 84%

Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

et al. 2006

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Although the secreted molecule Fgf8 is a key player of the isthmic organiser function, the mechanisms by which it acts remain unclear. Here, we present evidence indicating that Fgf8 is not instructive in establishing zebrafish cerebellar cell identities, although it is required for proliferation and morphogenesis of this territory. We first show that, as in mouse, lack of Otx function in zebrafish leads to transformation of the presumptive mesencephalon into an extended rhombomere 1 (r1). Expanded Fgf8 expression was proposed to be the cause of this fate transformation. However, this report demonstrates that zebrafish embryos lacking both Otx and fgf8 functions retain an extended r1 and display differentiation of at least two cerebellar cell fates. We show that this is not caused by presence of other Fgfs, which implies that in absence of Otx, Fgf function is not necessary for the differentiation of cerebellar cell types. Otx proteins are therefore potent repressors of cerebellar fates, kept out of r1 progeny by Fgf8. Because Otx transcripts are not present in presumptive r1 territory prior to fgf8 expression, Fgf8 is required to maintain, rather than induce, the posterior boundary of Otx expression. This maintenance is enough to allow cerebellar differentiation.

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Section: Transformation Of the Midbrain Into Rhombomere 1 In Otxh Mormentioning

confidence: 84%

Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

et al. 2006

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“…1J,L; n ϭ 20) was severely diminished. The expression of another bHLH factor atoh1b, which is expressed in a subset of atoh1a(ϩ) cells (Adolf et al, 2004), was also abolished (Fig. 1K,M).…”

Section: Two Distinct Classes Of Neurons In the Dorsal Hindbrain Basementioning

confidence: 94%

“…The bHLH transcription factor Atonal homolog 1 (Atoh1) is expressed in the rhombic lip, a germinal zone in the dorsal hindbrain of vertebrates from zebrafish to mouse (Köster and Fraser, 2001a;Adolf et al, 2004;Aruga, 2004;Wang et al, 2005). We performed in situ hybridization analysis of serial coronal sections of the hindbrain with the atoh1a and lhx2/9 probes and found that these two genes are expressed with a partial overlap in the dorsolateral hindbrain ( Fig.…”

Section: Two Distinct Classes Of Neurons In the Dorsal Hindbrain Basementioning

confidence: 99%

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Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems

Sassa

Aizawa

Okamoto

2007

Developmental Dynamics

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The dorsal hindbrain includes distinct classes of neurons for processing various sensory stimuli, but the developmental aspects of these neurons remain largely unknown. We identify here two distinct classes of neurons in the dorsal hindbrain of developing zebrafish: (1) neurons that express the inhibitory neuronal marker Gad1/2, and (2) neurons that express the zn-5 antigen and Lhx2/9 and require the basic helix-loop-helix transcription factor Atoh1a for development. Neurons were traced to their axon terminals by expressing green fluorescent protein using the Gal4VP16-UAS (UAS, upstream activating sequences) system in combination with the promoter/enhancer regions of gad2 for the Gad1/2(؉) neurons and zic1 for the zn-5(؉)Lhx2/9(؉) neurons. The Gad1/2(؉) neurons projected to the contralateral hindbrain, while the zn-5(؉)Lhx2/9(؉) neurons projected to the contralateral midbrain torus semicircularis, suggesting a role in auditory and lateral line sensory processing. Comparison of these projections with those from the cochlear nuclei to the inferior colliculus in mammals suggests similarities across vertebrate species. Developmental Dynamics 236:706 -718, 2007.

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“…The four target genes selected, sp8l and'sp9 (two members of the Sp1 family of zinc-finger transcription factors (Kawakami et al, 2004), dmbx1a (a homeobox-encoding gene, ( (Kawahara et al, 2002); also known as mbx) and bhlhb5 (a bHLH-encoding gene, (Adolf et al, 2004), all encode transcription factors and are all transcribed in presumptive rhombencephalic tissues. These tissues are patterned by transcription factor networks in which hox genes exert a central function.…”

Section: Spatially Restricted Expression Of Some Hoxb1b/hoxb1a Inducementioning

confidence: 99%

Identification of hoxb1b downstream genes: hoxb1b as a regulatory factor controlling transcriptional networks and cell movement during zebrafish gastrulation

Akker¹,

Durston²,

Spaink³

2010

Int. J. Dev. Biol.

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Hox proteins are homeobox containing transcription factors that play important roles in patterning the presumptive central nervous system and the axial mesoderm in the early vertebrate embryo. Hox genes are first expressed during gastrula stages and recent studies suggest that their function goes beyond their role as patterning determinants. To improve our understanding of the role of Hox proteins during early vertebrate development, we designed a strategy to identify target genes of the zebrafish hoxb1b using overexpression and whole-genome microarray analysis. We directly compared the hoxb1b microarray data with those resulting from heterologous over-expression of the Xenopus XhoxD1 gene in zebrafish embryos. Both genes are the first expressed hox genes in their respective native embryos and display similar spatial expression patterns. The zebrafish transcriptome was analysed prior to the start of the expression of the endogenous hoxb1b gene and we observed extensive overlap between the hoxb1b and XhoxD1 putative downstream genes suggesting evolutionary functional conservation between these hox genes. Furthermore, genes encoding transcription factors and proteins that are known to be involved in cell adhesion and movement were over-represented among the candidate downstream genes, indicating the involvement of the developmentally earliest expressed hox genes in transcriptional networks and cell movement processes.

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atoh1.2 and beta3.1 are two new bHLH-encoding genes expressed in selective precursor cells of the zebrafish anterior hindbrain

Cited by 36 publications

References 11 publications

Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems

Identification of hoxb1b downstream genes: hoxb1b as a regulatory factor controlling transcriptional networks and cell movement during zebrafish gastrulation

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