Visualization of two distinct classes of neurons by <i>gad2</i> and <i>zic1</i> promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems

Sassa, Takayuki; Aizawa, Hidenori; Okamoto, Hitoshi

doi:10.1002/dvdy.21084

Cited by 24 publications

(27 citation statements)

References 56 publications

(124 reference statements)

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“…In a divergent neural submap, each neuron forms ϳ60 boutons that concentrate a well characterized presyn- aptic marker. If we assume that each bouton represents a synaptic contact with one output neuron, the posterior lateral line has no more than 2,400 second-order targets in the zebrafish larva (Fame et al, 2006;Sassa et al, 2007). In a convergent submap, many lateralis neurons directly contact the lateral dendrite of the Mauthner cell.…”

Section: Discussionmentioning

confidence: 99%

Neuronal Birth Order Identifies a Dimorphic Sensorineural Map

Pujol-Martí¹,

Zecca²,

Baudoin³

et al. 2012

J. Neurosci.

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Spatially distributed sensory information is topographically mapped in the brain by point-to-point correspondence of connections between peripheral receptors and central target neurons. In fishes, for example, the axonal projections from the mechanosensory lateral line organize a somatotopic neural map. The lateral line provides hydrodynamic information for intricate behaviors such as navigation and prey detection. It also mediates fast startle reactions triggered by the Mauthner cell. However, it is not known how the lateralis neural map is built to subserve these contrasting behaviors. Here we reveal that birth order diversifies lateralis afferent neurons in the zebrafish. We demonstrate that early-and late-born lateralis afferents diverge along the main axes of the hindbrain to synapse with hundreds of second-order targets. However, early-born afferents projecting from primary neuromasts also assemble a separate map by converging on the lateral dendrite of the Mauthner cell, whereas projections from secondary neuromasts never make physical contact with the Mauthner cell. We also show that neuronal diversity and map topology occur normally in animals permanently deprived of mechanosensory activity. We conclude that neuronal birth order correlates with the assembly of neural submaps, whose combination is likely to govern appropriate behavioral reactions to the sensory context.

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Section: Discussionmentioning

confidence: 99%

Neuronal Birth Order Identifies a Dimorphic Sensorineural Map

Pujol-Martí¹,

Zecca²,

Baudoin³

et al. 2012

J. Neurosci.

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“…As stated in the Introduction section, we became aware that the previous studies employing "GAL4VP16" in zebrafish (Davison et al, 2007;Inbal et al, 2006;Köster and Fraser, 2001;Sagasti et al, 2005;Sassa et al, 2007;Scott et al, 2007) actually used GAL4VP16 (Barlev et al, 1995) instead of the original GAL4VP16 construct (Sadowski et al, 1988). Direct give reporter expression…”

Section: Modified Gal4 Activators With Lower Toxicity Show a High Tramentioning

confidence: 99%

“…3). Toxicity has not been described in transgenic zebrafish lines that express GAL4VP16 413-470 under the control of tissue-specific regulatory elements from the genes isl1, gsc, gad2, and zic1 (Inbal et al, 2006;Sagasti et al, 2005;Sassa et al, 2007). Davison et al (2007) also used GAL4VP16 in their gene-trap constructs without observing deleterious effects.…”

Section: Alleviation Of Gal4vp16 Toxicity Through Alterations In the mentioning

confidence: 99%

“…Grabher and Wittbrodt (2004) employed the fulllength VP16 activation domain in their fusion construct as the original GAL4VP16 construct (Sadowski et al, 1988). However, the other studies that employed "GAL4VP16" in zebrafish (Davison et al, 2007;Inbal et al, 2006;Köster and Fraser, 2001;Sagasti et al, 2005;Sassa et al, 2007;Scott et al, 2007) actually used GAL4VP16 (Barlev et al, 1995) instead of the original GAL4VP16 construct (Sadowski et al, 1988), although this fact was not explicit in these reports (personal communication from Dr. Köster, who first used GAL4VP16 in zebrafish; Köster and Fraser, 2001). In GAL4VP16 , the truncated VP16 activation domain (aa 413-470) was fused to GAL4 DBD in place of the full activation domain (aa 413-490).…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish

Ogura

Okuda

Kondoh

et al. 2009

Developmental Dynamics

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An enhancer trap-based GAL4-UAS system in zebrafish requires strong GAL4 activators with minimal adverse effects. However, the activity of yeast GAL4 is too low in zebrafish, while a fusion protein of the GAL4 DNA-binding domain and the VP16 activation domain is toxic to embryonic development, even when expressed at low levels. To alleviate this toxicity, we developed variant GAL4 activators by fusing either multimeric forms of the VP16 minimal activation domain or the NF-B activation domain to the GAL4 DNA-binding domain. These variant GAL4 activators are sufficiently innocuous and yet highly effective transactivators in developing zebrafish. Enhancer-trap vectors containing these GAL4 activators downstream of an appropriate weak promoter were randomly inserted into the zebrafish genome using the Sleeping Beauty transposon system. By the combination of these genetic elements, we have successfully developed enhancer trap lines that activate UAS-dependent reporter genes in a tissue-specific fashion that reflects trapped enhancer activities. Developmental Dynamics 238:641-655, 2009.

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“…Neurons that derive from the same neuroblast population could express a different combination of proteins depending on their position along a morphogen gradient, whose spatial distribution could vary over time as the brain enlarges. The generation of transgenic animals that label candidate second-order neurons in the MON will allow for testing of this possibility in vivo (Fame et al, 2006;Sassa et al, 2007;Scott and Baier, 2009). Regardless of the mechanism involved, our results provide a framework within which to assess the impact of timing in the progression of somatotopy.…”

Section: Developmental Dynamicsmentioning

confidence: 99%

Progressive neurogenesis defines lateralis somatotopy

Pujol-Martí¹,

Baudoin²,

Faucherre³

et al. 2010

Developmental Dynamics

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Fishes and amphibians localize hydromechanical variations along their bodies using the lateral-line sensory system. This is possible because the spatial distribution of neuromasts is represented in the hindbrain by a somatotopic organization of the lateralis afferent neurons' central projections. The mechanisms that establish lateralis somatotopy are not known. Using BAPTI and neuronal tracing in the zebrafish, we demonstrate growth anisotropy of the posterior lateralis ganglion. We characterized a new transgenic line for in vivo imaging to show that although peripheral growth-cone structure adumbrates somatotopy, the order of neurogenesis represents a more accurate predictor of the position of a neuron's central axon along the somatotopic axis in the hindbrain. We conclude that progressive neurogenesis defines lateralis somatotopy.

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Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems

Cited by 24 publications

References 56 publications

Neuronal Birth Order Identifies a Dimorphic Sensorineural Map

Neuronal Birth Order Identifies a Dimorphic Sensorineural Map

Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish

Progressive neurogenesis defines lateralis somatotopy

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