Background: Recently, atmospheric Low Temperature Plasma(LTP) has attracted attention as a novel medical tool that might be useful for achieving hemostasis. However, conventional plasma sources are too big for use with endoscopes, and the efficacy of LTP for achieving hemostasis in cases of gastrointestinal bleeding is difficult to investigate. In this study, to solve the problem, we developed a 3D printed LTP jet which has a diameter of 2.8 mm and metal body for endoscopic use And the characteristics, hemostasis efficacy and safety were investigated. Materials and Methods: As investigation of the basic characteristics of the developed plasma jet, the electron densities, gas temperatures, and reactive species were measured by emission spectroscopy and thermocouple To evaluate the efficacy of such hemostatic treatment, porcine gastrointestinal bleeding was treated with the device. In addition, to investigate the safety of such treatment, the CO LTP-treated tissue was compared with tissue that was treated with clipping-or argon plasma coagulation (APC)-based hemostasis for 5 days, and hematoxylin and eosin staining was used to evaluate tissue damage in the treated regions. Results: The measurement of emission spectroscopy, the power, and the electron density of various gas plasmas suggested that a high density (10 cm) LTP of CO was generated by the LTP jet and the gas temperature was 41.5°C at 3 mm from the outlet ofLTP. he CO LTP achieved hemostasis of oozing blood with 0±2 s. In addition, the CO LTP gave earlier recovery than clipping-or APC-based hemostasis, and the treated regions ha no damage by the CO LTP treatment. Conclusions: These results indicated that the developed LTP plasma jet has potential to be used for endoscopic hemostasis.