Plasma Gas Temperature Control Performance of Metal 3D-Printed Multi-Gas Temperature-Controllable Plasma Jet

Suenaga, Yuma; Takamatsu, Takeichiro; Aizawa, Toshiki; Moriya, Shohei; Matsumura, Yukihiko; Iwasawa, Atsuo; Okino, Akitoshi

doi:10.3390/app112411686

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…The plasma jet device was grounded, and the internal high-voltage electrode was connected to an AC power supply (Plasma Concept Tokyo Inc.) of 16 kHz and 9 kV at approximately 10 W. Temperature-controlled atmospheric-pressure plasma was generated from nitrogen (N 2 ) or carbon dioxide (CO 2 ) and cooled by a gas-cooling system using a temperature-controlled fluid. The plasma was ejected from a 1 mm aperture of the plasma jet device at a flow rate of 5 L/min [ 19 – 21 ].…”

Section: Methodsmentioning

confidence: 99%

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma

et al. 2023

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Previously, we developed a technique to introduce a superfolder green fluorescent protein (sGFP) fusion protein directly into plant cells using atmospheric-pressure plasma. In this study, we attempted genome editing using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system using this protein introduction technique. As an experimental system to evaluate genome editing, we utilized transgenic reporter plants carrying the reporter genes L-(I-SceI)-UC and sGFP-waxy-HPT. The L-(I-SceI)-UC system allowed the detection of successful genome editing by measuring the chemiluminescent signal observed upon re-functionalization of the luciferase (LUC) gene following genome editing. Similarly, the sGFP-waxy-HPT system conferred hygromycin resistance caused by hygromycin phosphotransferase (HPT) during genome editing. CRISPR/Cas9 ribonucleoproteins targeting these reporter genes were directly introduced into rice calli or tobacco leaf pieces after treatment with N2 and/or CO2 plasma. Cultivation of the treated rice calli on a suitable medium plate produced the luminescence signal, which was not observed in the negative control. Four types of genome-edited sequences were obtained upon sequencing the reporter genes of genome-edited candidate calli. sGFP-waxy-HPT-carrying tobacco cells exhibited hygromycin resistance during genome editing. After repeated cultivation of the treated tobacco leaf pieces on a regeneration medium plate, the calli were observed with leaf pieces. A green callus that was hygromycin-resistant was harvested, and a genome-edited sequence in the tobacco reporter gene was confirmed. As direct introduction of the Cas9/sgRNA (single guide RNA) complex using plasma enables genome editing in plants without any DNA introduction, this method is expected to be optimized for many plant species and may be widely applied for plant breeding in the future.

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Section: Methodsmentioning

confidence: 99%

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma

et al. 2023

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“…Ensuring a uniform airflow in each flow channel of the ten-layer DBD reactor is indispensable for achieving stable and homogeneous plasma generation in each layer, thereby maximizing the optimum performance. Therefore, fluid dynamics simulations were conducted during the process of designing a ten-layer, high-flow-rate DBD reactor to gain insight into the fluid dynamics of the flow path [39][40][41]. The Autodesk CFD simulation software (Autodesk CFD 2023 23.0, Autodesk Inc., San Rafael, CA, USA) was used.…”

Section: Cfd Simulation-assisted Flow Path Designmentioning

confidence: 99%

“…To verify the velocity field of the 10 channels in the ten-layer DBD reactor, the governing equations describing the conservation of mass and momentum are as follows; the continuity and Navier-Stokes equations for an incompressible flow were discretized and solved using the finite element method (FEM). Because the fluid flow introduced into the ten-layer DBD reactor was treated as a turbulent incompressible flow in this study, the continuity equation can be written as follows [40][41][42]:…”

Section: Cfd Simulation-assisted Flow Path Designmentioning

confidence: 99%

Design and Characterization of an Upscaled Dielectric Barrier Discharge-Based Ten-Layer Plasma Source for High-Flow-Rate Gas Treatment

Xu,

Mori,

Liu

et al. 2023

Applied Sciences

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Dielectric barrier discharge (DBD)-based technology is considered a promising alternative for controlling indoor air pollutants. However, its limited processing capacity and lack of design techniques have restricted its use in practical applications. This paper introduces a methodology for designing upscaled DBD reactors with a processing capacity of up to 1000 L/min for treating high-flow-rate gases to mitigate indoor air pollution. A ten-layer high-flow-rate DBD reactor was constructed, with fundamental characterizations, including electrical and spectroscopic measurements, conducted to verify the feasibility of the proposed methodology. In particular, the flow paths of the ten-layer DBD reactor were optimized by incorporating an air diffuser and perforated metal plates, all without significant modifications. Computational fluid dynamics simulations showed a remarkably improved velocity uniformity (0.35 m/s to 0.04 m/s, as evidenced by the velocity standard deviation) in the 10 flow channels. These simulation results were consistent with the experimental results, wherein the velocity standard deviation reduced from 1.38 m/s to 0.13 m/s. Moreover, multi-gas plasma ignition for up to six gas species and high-flow-rate plasma generation of up to 1000 L/min were achieved. These results provide the foundation for developing DBD technologies for practical applications in high-flow-rate gas treatment, particularly for controlling indoor air pollution.

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“…Contributions to this Special Issue focus on different aspects of Atmospheric-Pressure Plasma Technology, giving valuable examples of applied research in the field. This Special Issue of Applied Sciences, "Recent Advances in Atmospheric-Pressure Plasma Technology", includes one review [3] and eight original papers [4][5][6][7][8][9][10][11], providing new insights into the application of atmospheric pressure plasma technology. Domonkos et al [3] discussed the application of cold atmospheric pressure plasma (CAPP) technology in different configurations.…”

Section: Review Of Special Issue Contentsmentioning

confidence: 99%

“…The results demonstrate that the plasma gas type and temperature have a significant influence on the reactive species produced, and the bactericidal effect of plasma and the disinfection process could be improved by properly selecting the plasma gas species and temperature. Suenaga et al [8] design and build a multi-gas temperature-controllable plasma jet that can adjust the gas temperature of plasmas with various gas species and evaluated its temperature control performance. By varying the plasma jet body temperature from −30 • C to 90 • C, the gas temperature was successfully controlled linearly in the range of 29-85 • C for all plasma gas species.…”

Section: Review Of Special Issue Contentsmentioning

confidence: 99%

Recent Advances in Atmospheric-Pressure Plasma Technology

Rusu¹

2022

Applied Sciences

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Plasma Gas Temperature Control Performance of Metal 3D-Printed Multi-Gas Temperature-Controllable Plasma Jet

Cited by 5 publications

References 23 publications

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma

Design and Characterization of an Upscaled Dielectric Barrier Discharge-Based Ten-Layer Plasma Source for High-Flow-Rate Gas Treatment

Recent Advances in Atmospheric-Pressure Plasma Technology

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