Atmospheric H2S as sulphur source for Brassica oleracea: Consequences for the activity of the enzymes of the assimilatory sulphate reduction pathway

Westerman, S.; Stulen, I.; Suter, Marianne; Brunold, Christian; Kok, Luit J. De

doi:10.1016/s0981-9428(01)01258-x

Cited by 76 publications

(44 citation statements)

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“…1). The increased APR activity and GSH level in salt-treated roots correlate with previous observations of demand-driven regulation of sulfate assimilation and the key role of APR in control of the pathway (Brunner et al, 1995;Lappartient and Touraine, 1996; Lee and Leustek, 1999; Leustek et al, 2000; Westerman et al, 2001;Vauclare et al, 2002;Kocsy et al, 2004;Kopriva, 2006). In fact, other enzymes of sulfate assimilation are also induced by salt, including ATP sulfurylase, Ser acetyltransferase, and a cytosolic isoform of OASTL (Barroso et al, 1999;Ruiz and Blumwald, 2002).…”

Section: Discussionsupporting

confidence: 85%

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Complex Signaling Network in Regulation of Adenosine 5′-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots

2008

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Sulfur-containing compounds play an important role in plant stress defense; however, only a little is known about the molecular mechanisms of regulation of sulfate assimilation by stress. Using known Arabidopsis (Arabidopsis thaliana) mutants in signaling pathways, we analyzed regulation of the key enzyme of sulfate assimilation, adenosine 5′-phosphosulfate reductase (APR), by salt stress. APR activity and mRNA levels of all three APR isoforms increased 3-fold in roots after 5 h of treatment with 150 mm NaCl. The regulation of APR was not affected in mutants deficient in abscisic acid (ABA) synthesis and treatment of the plants with ABA did not affect the mRNA levels of APR isoforms, showing that APR is regulated by salt stress in an ABA-independent manner. In mutants deficient in jasmonate, salicylate, or ethylene signaling, APR mRNA levels were increased upon salt exposure similar to wild-type plants. Surprisingly, however, APR enzyme activity was not affected by salt in these plants. The same result was obtained in mutants affected in cytokinin and auxin signaling. Signaling via gibberellic acid, on the other hand, turned out to be essential for the increase in APR mRNA by salt treatment. These results demonstrate an extensive posttranscriptional regulation of plant APR and reveal that the sulfate assimilation pathway is controlled by a complex network of multiple signals on different regulatory levels.

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Section: Discussionsupporting

confidence: 85%

“…Nikiforova et al, 2003). On the other hand, a surplus of reduced sulfur compounds by fumigation with H 2 S or by feeding thiols represses the pathway (Westerman et al, 2001;Vauclare et al, 2002). The key regulatory steps of sulfate assimilation are the transport of sulfate into the cells and the reduction of APS to sulfite by APR (Vauclare et al, 2002).…”

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Complex Signaling Network in Regulation of Adenosine 5′-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots

2008

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“…In rapeseed (Brassica napus) seedlings, split-root experiments showed that glutathione acts as a long-distance signal from the shoot to the root: the negative regulation of sulfate uptake resulting from the application of glutathione in one part of the root system was also demonstrated in the untreated part of the root (Lappartient and Touraine, 1996). However, such a negative effect of glutathione on the root sulfate uptake capacity was not observed in poplar after an exposure of aerial organs to H 2 S, although this treatment was shown to significantly increase leaf and root glutathione contents (Herschbach et al, 2000;Westerman et al, 2001). Also, the overexpression of the key enzyme for glutathione synthesis, g-glutamyl-Cys synthase, which resulted in the overaccumulation of glutathione, did not induce any down-regulation of the expression of sulfate transporters (Herschbach et al, 2000;Hartmann et al, 2004).…”

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Differential Regulation of the Expression of Two High-Affinity Sulfate Transporters, SULTR1.1 and SULTR1.2, in Arabidopsis

et al. 2008

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The molecular mechanisms regulating the initial uptake of inorganic sulfate in plants are still largely unknown. The current model for the regulation of sulfate uptake and assimilation attributes positive and negative regulatory roles to O-acetyl-serine (O-acetyl-Ser) and glutathione, respectively. This model seems to suffer from exceptions and it has not yet been clearly validated whether intracellular O-acetyl-Ser and glutathione levels have impacts on regulation. The transcript level of the two high-affinity sulfate transporters SULTR1.1 and SULTR1.2 responsible for sulfate uptake from the soil solution was compared to the intracellular contents of O-acetyl-Ser, glutathione, and sulfate in roots of plants submitted to a wide diversity of experimental conditions. SULTR1.1 and SULTR1.2 were differentially expressed and neither of the genes was regulated in accordance with the current model. The SULTR1.1 transcript level was mainly altered in response to the sulfur-related treatments. Split-root experiments show that the expression of SULTR1.1 is locally regulated in response to sulfate starvation. In contrast, accumulation of SULTR1.2 transcripts appeared to be mainly related to metabolic demand and is controlled by photoperiod. On the basis of the new molecular insights provided in this study, we suggest that the expression of the two transporters depends on different regulatory networks. We hypothesize that interplay between SULTR1.1 and SULTR1.2 transporters could be an important mechanism to regulate sulfate content in the roots.

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“…In the absence of pedospheric sulfate, plants may grow with H 2 S as the sole S source for growth , 2000Westerman et al, 2000a). H 2 S exposure of B. oleracea resulted in a negative feedback regulation of the uptake and assimilation of pedospheric sulfate (Westerman et al, 2000a(Westerman et al, , 2000b(Westerman et al, , 2001a(Westerman et al, , 2001b.…”

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Regulation of Sulfate Uptake and Expression of Sulfate Transporter Genes in Brassica oleracea as Affected by Atmospheric H2S and Pedospheric Sulfate Nutrition

et al. 2004

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Demand-driven signaling will contribute to regulation of sulfur acquisition and distribution within the plant. To investigate the regulatory mechanisms pedospheric sulfate and atmospheric H 2 S supply were manipulated in Brassica oleracea. Sulfate deprivation of B. oleracea seedlings induced a rapid increase of the sulfate uptake capacity by the roots, accompanied by an increased expression of genes encoding specific sulfate transporters in roots and other plant parts. More prolonged sulfate deprivation resulted in an altered shoot-root partitioning of biomass in favor of the root. B. oleracea was able to utilize atmospheric H 2 S as S-source; however, root proliferation and increased sulfate transporter expression occurred as in S-deficient plants. It was evident that in B. oleracea there was a poor shoot to root signaling for the regulation of sulfate uptake and expression of the sulfate transporters. cDNAs corresponding to 12 different sulfate transporter genes representing the complete gene family were isolated from Brassica napus and B. oleracea species. The sequence analysis classified the Brassica sulfate transporter genes into four different groups. The expression of the different sulfate transporters showed a complex pattern of tissue specificity and regulation by sulfur nutritional status. The sulfate transporter genes of Groups 1, 2, and 4 were induced or up-regulated under sulfate deprivation, although the expression of Group 3 sulfate transporters was not affected by the sulfate status. The significance of sulfate, thiols, and O-acetylserine as possible signal compounds in the regulation of the sulfate uptake and expression of the transporter genes is evaluated.

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Atmospheric H2S as sulphur source for Brassica oleracea: Consequences for the activity of the enzymes of the assimilatory sulphate reduction pathway

Cited by 76 publications

References 23 publications

Complex Signaling Network in Regulation of Adenosine 5′-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots

Complex Signaling Network in Regulation of Adenosine 5′-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots

Differential Regulation of the Expression of Two High-Affinity Sulfate Transporters, SULTR1.1 and SULTR1.2, in Arabidopsis

Regulation of Sulfate Uptake and Expression of Sulfate Transporter Genes in Brassica oleracea as Affected by Atmospheric H2S and Pedospheric Sulfate Nutrition

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