ATL: A Preclinical Model of Spontaneous Ovarian Cancer

Barua, Animesh; Yellapa, Aparna; Bahr, Janice M.; Machado, Sérgio Luiz de Oliveira; Bitterman, Pincas; Basu, Sanjib; Sharma, Sameer; Abramowicz, Jacques S.

doi:10.1097/igc.0000000000000040

Cited by 19 publications

(8 citation statements)

References 38 publications

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“…Furthermore, this study also showed that the density of IL-16 expressing microvessels increased significantly with the development of OVCA and increased further as the tumor progressed to late stages. The frequencies of tumor-associated microvessels expressing α v β 3 -integrins and VEGFR-2 have also been suggested to increase contrast enhanced ultrasound signal intensities [ 32 , 33 ]. Thus, in addition to malignant cells, increase in the frequency of IL-16 expressing microvessel might also be a reason for the increased signal intensities during contrast enhanced imaging in hens with OVCA.…”

Section: Discussionmentioning

confidence: 99%

Interleukin 16- (IL-16-) Targeted Ultrasound Imaging Agent Improves Detection of Ovarian Tumors in Laying Hens, a Preclinical Model of Spontaneous Ovarian Cancer

Barua

Yellapa

Bahr

et al. 2015

BioMed Research International

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Limited resolution of transvaginal ultrasound (TVUS) scanning is a significant barrier to early detection of ovarian cancer (OVCA). Contrast agents have been suggested to improve the resolution of TVUS scanning. Emerging evidence suggests that expression of interleukin 16 (IL-16) by the tumor epithelium and microvessels increases in association with OVCA development and offers a potential target for early OVCA detection. The goal of this study was to examine the feasibility of IL-16-targeted contrast agents in enhancing the intensity of ultrasound imaging from ovarian tumors in hens, a model of spontaneous OVCA. Contrast agents were developed by conjugating biotinylated anti-IL-16 antibodies with streptavidin coated microbubbles. Enhancement of ultrasound signal intensity was determined before and after injection of contrast agents. Following scanning, ovarian tissues were processed for the detection of IL-16 expressing cells and microvessels. Compared with precontrast, contrast imaging enhanced ultrasound signal intensity significantly in OVCA hens at early (P < 0.05) and late stages (P < 0.001). Higher intensities of ultrasound signals in OVCA hens were associated with increased frequencies of IL-16 expressing cells and microvessels. These results suggest that IL-16-targeted contrast agents improve the visualization of ovarian tumors. The laying hen may be a suitable model to test new imaging agents and develop targeted anti-OVCA therapeutics.

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Section: Discussionmentioning

confidence: 99%

Interleukin 16- (IL-16-) Targeted Ultrasound Imaging Agent Improves Detection of Ovarian Tumors in Laying Hens, a Preclinical Model of Spontaneous Ovarian Cancer

Barua

Yellapa

Bahr

et al. 2015

BioMed Research International

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“…For evaluating tumour growth non-invasively, successful in vivo quantification of the expression levels of the angiogenetic biomarkers a v b 3 integrin, endoglin, and VEGFR2, which vary during tumour growth in subcutaneous cancer xenografts, have been reported [158]. Recently, an UMI study using MBs targeted to a v b 3 in an ovarian cancer model in hens [160], suggested that the detection of ovarian tumour-associated angiogenic microvessels improved when using UMI. For assessing the efficacy of cell-based therapies, UMI has been used to image a genetically engineered cell-surface marker on endothelial progenitor cells to track the fate of these progenitor cells after their delivery into vascular engraftment in vivo within Matrigel plugs [161].…”

Section: In Vivo Molecular Imagingmentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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“…Progress in understanding a disease or cancer, as well as in developing cures for that disease or cancer, requires an appropriate animal model. Using the chicken, we are developing more sensitive methods of ultrasonography, allowing ovarian cancer to be identified in stage 1 or 2, when survival is very high (34)(35)(36). The chicken is also an excellent model to test dietary interventions for the reduction or elimination of cancer (37,38).…”

Section: The Chicken: the Perfect Animal Model To Study Human Ovarianmentioning

confidence: 99%

My Scientific Journey: From an Agrarian Start to an Academic Setting

Bahr

2017

Annu. Rev. Anim. Biosci.

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This article is a combination of an autobiography and a review of outstanding research done by over 70 graduate students, postdoctoral fellows, and visiting scientists along with excellent collaborators during my over-40-year career as a professor of reproductive physiology at the University of Illinois, UrbanaChampaign. I have also shared thoughts on mentoring, how research has changed over the years, and the future of reproductive physiology. I provide the reader with a snapshot of the challenges faced by a woman eager to obtain a PhD under the guidance of renowned professors in the early 1970s and to be hired as the first woman, and the only permanent female faculty member, for more than 20 years on a faculty of 40 men. As a comparative reproductive physiologist, I describe the various animal models used because they were the best models to answer specific questions in reproduction. Also, my graduate students and postdoctoral fellows were given the freedom to identify their research topics, articulate hypotheses to be tested, and select appropriate animal models. This approach caused students to take ownership of their research, resulting in the development of independent and creative scientists and over 170 publications, excluding chapters in top-tier journals. Finally, I am so grateful for a truly rich life mentoring graduate students and postdoctoral fellows who have become my lifelong friends.

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ATL: A Preclinical Model of Spontaneous Ovarian Cancer

Abstract: These results suggest that αvβ3 integrin-targeted imaging agent enhanced the visualization of ovarian tumor-associated angiogenic microvessels in hens with early-stage OVCA and may form a foundation for clinical studies.

Cited by 19 publications

References 38 publications

Interleukin 16- (IL-16-) Targeted Ultrasound Imaging Agent Improves Detection of Ovarian Tumors in Laying Hens, a Preclinical Model of Spontaneous Ovarian Cancer

Interleukin 16- (IL-16-) Targeted Ultrasound Imaging Agent Improves Detection of Ovarian Tumors in Laying Hens, a Preclinical Model of Spontaneous Ovarian Cancer

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

My Scientific Journey: From an Agrarian Start to an Academic Setting

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