Atividade in vitro do óleo essencial de Origanum vulgare frente à Sporothrix Schenckii

Abstract

Atividade in vitro do óleo essencial de Origanum vulgare frente à Sporothrix Schenckii [In vitro 3 , e caracterizado em função do conteúdo de compostos fenólicos, timol e/ou carvacrol. Para isso, foi preparada uma solução do óleo a 5000mg/l em hexano e soluções de 40mg/l dos padrões: α-pineno, canfeno, β-pineno, mirceno, α-terpinemo, p-cimeno, limoneno, 1,8-cineol, γ-terpineno, terpinoleno, linalol, 4-terpineol, α-terpineol, timol, carvacrol, das quais foram injetadas 1µl. Os constituintes foram identificados por comparação entre o tempo de retenção desses padrões e das amostras.

Anti- Sporothrix spp. activity of medicinal plants

“…activity through tests of broth microdilution in standard strains isolated from humans and felines (Verástegui et al, 1996;Johann et al, 2007;Luqman et al, 2007;Cleff et al, 2008;Fatima et al, 2009;Johann et al, 2011;Joshi et al, 2011;Stopiglia et al, 2011). This method is based on the proportion of microbial growth in liquid medium challenged with the concentration of the measured substance using a 96-well microplate (CLSI, 2002;Pinto et al, 2003).…”

“…activity was conferred in 100 species belonging to 39 botanical families, in which were highlighted the families of Combretaceae (31% -31/100) (Masoko, Picard, Eloff, 2005;2007;Suleiman et al, 2009), Asteraceae (11% -11/100) (Rojas et al, 2003;Damián-Badillo et al, 2008;Salomão et al, 2008;Stopiglia et al, 2011) and Lamiaceae (7% -07/100) (Sinha, Gulati, 1990;Luqman et al, 2007;Fernández, 2005;Cleff et al, 2008;Couto et al, 2015), which have the largest number of species with confirmed activity of interest. From the in vitro studies revised, a total of 233 extract products showed anti-Sporothrix spp.…”

“…In the studies reviewed, the methodology utilized for research on plants were agar diffusion (Apisariyakul et al, 1995;Saikia et al, 2001;Rojas et al, 2003;Luqman et al, 2007;Damián-Badillo et al, 2008;Verástegui et al, 2008;Fatima et al, 2009;Márquez, 2010;Joshi et al, 2011), broth microdilution technique (Sinha, Gulati, 1990;Verástegui et al, 1996;Johann et al, 2007;Luqman et al, 2007;Cleff et al, 2008;Fatima et al, 2009;Johann et al, 2009Johann et al, , 2011Joshi et al, 2011), bioautographic assay (Betina, 1973;Johann et al, 2007;, serial microdilution at 24 and 48 hours (Masoko, Picard, Eloff, 2005;2007;Suleiman et al, 2009) and agar cup method (Salomão et al, 2008).…”

“…There is a continuing and urgent need to discover new antifungal compounds and to understand their mechanisms of action (Kontoyiannis, Lewis, 2002;Cleff et al, 2008;Gaitán et al 2011). It is known that the use of plants and derivatives for medicinal purposes is an ancient practice used worldwide, primarily in Eastern cultures, and is utilized by up to 80% of the population of developing countries and has been increasingly used in the West, according to data from the World Health Organization in the 1990's (Veiga Jr., Pinto, 2005).…”

“…of certain medicinal plants (Rojas et al, 2003;Masoko, Picard, Eloff, 2007;Cleff et al, 2008;Suleiman et al, 2009;Johann et al, 2011;Joshi et al, 2011;Stopiglia et al, 2011). However, there is a lack of standardization of tests with medicinal plants and this makes the comparison of antifungal activity of the plants difficult.…”