AtCCMA Interacts with AtCcmB to Form a Novel Mitochondrial ABC Transporter Involved in Cytochrome c Maturation in Arabidopsis

Rayapuram, Naganand; Hagenmuller, Jérémie; Grienenberger, Jean‐Michel; Giegé, Philippe; Bonnard, Géraldine

doi:10.1074/jbc.m704091200

Cited by 50 publications

(47 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Arabidopsis CCMA protein was shown to face the matrix side of mitochondrial membranes and interacted with CcmB in yeast two-hybrid studies. Recombinant CCMA protein had ATPase activity [58]. From these data it can be assumed that the plant mitochondrial CcmAB is analogous to the better characterized homologue in E. coli, although the substrate of the transporter is not yet known.…”

Section: Ccmab In Plant Mitochondriamentioning

confidence: 94%

“…The plant ABC transporter of the Ccm pathway has retained its prokaryotic structure in that the TMD and NBD domains are encoded by separate genes. Interestingly, the ccmB gene (ABCI2) encoding the TMD is located on the mitochondrial genome, whereas the CCMA gene (ABCI1) for the NBD is encoded in the nucleus of higher plants [58]. The Arabidopsis CCMA protein was shown to face the matrix side of mitochondrial membranes and interacted with CcmB in yeast two-hybrid studies.…”

Section: Ccmab In Plant Mitochondriamentioning

confidence: 99%

See 1 more Smart Citation

Structures and functions of mitochondrial ABC transporters

Schaedler

Faust

Shintre

et al. 2015

Biochemical Society Transactions

View full text Add to dashboard Cite

A small number of physiologically important ATP-binding cassette (ABC) transporters are found in mitochondria. Most are half transporters of the B group forming homodimers and their topology suggests they function as exporters. The results of mutant studies point towards involvement in iron cofactor biosynthesis. In particular, ABC subfamily B member 7 (ABCB7) and its homologues in yeast and plants are required for iron-sulfur (Fe-S) cluster biosynthesis outside of the mitochondria, whereas ABCB10 is involved in haem biosynthesis. They also play a role in preventing oxidative stress. Mutations in ABCB6 and ABCB7 have been linked to human disease. Recent crystal structures of yeast Atm1 and human ABCB10 have been key to identifying substrate-binding sites and transport mechanisms. Combined with in vitro and in vivo studies, progress is being made to find the physiological substrates of the different mitochondrial ABC transporters. Sequence analysis of mitochondrial ABC transportersMitochondria of most eukaryote species harbour 2-4 different ABC transporters that belong to the B subfamily of half transporters. The proteins contain an N-terminal transmembrane domain (TMD) and a C-terminal nucleotidebinding domain (NBD), which form homodimers for full functionality [1]. Plant and algal genomes additionally encode a unique ABC transporter, termed cytochrome c maturation (Ccm)AB, in which the TMD and NBD are encoded by separate genes. CcmAB is placed in the ABCI subfamily, see section 'CcmAB in plant mitochondria'. Phylogenetic relationship of mitochondrial transporters of the ABCB subfamilyAs a starting point for the discussion of recent crystal structures and functional data, we have reanalysed the phylogenetic relationships of B-type mitochondrial ABC transporters and their closest prokaryotic homologues (Figure 1). The CcmAB proteins were excluded from the phylogenetic analysis due to their large evolutionary distance from the other mitochondrial ABC transporters.Key words: ATP-binding cassette (ABC) transporter, glutathione, haem, iron, mitochondria, oxidative stress. Abbreviations: ABC, ATP-binding cassette; Aft, Activator of ferrous transport; ATM, ABC transporter of the mitochondria; Ccm, cytochrome c maturation; dALA, δ-amino levulinic acid; IRP1, iron regulatory protein 1; Mdl, multi-drug resistance-like; Mfrn, mitoferrin; NBD, nt-binding domain; PPIX, protoporphyrin IX; TMD, transmembrane domain; TMH, transmembrane helix. 1 To whom correspondence should be addressed (email janneke.balk@jic.ac.uk).The ABCB7 group, which includes the ABC transporters of the mitochondria Atm1 in yeast and ATM3 in Arabidopsis, can be found in virtually all eukaryotic species. Human ABCB7 and Arabidopsis ATM3 can largely rescue the phenotypes of yeast atm1 mutants [2][3][4], providing experimental support that members of this subfamily may have the same function. Human ABCB6 can also partially complement a yeast atm1 mutant [5] but differences in the intracellular localization of ABCB6 and Atm1 may point to distinct phys...

show abstract

Section: Ccmab In Plant Mitochondriamentioning

confidence: 94%

Section: Ccmab In Plant Mitochondriamentioning

confidence: 99%

Structures and functions of mitochondrial ABC transporters

Schaedler

Faust

Shintre

et al. 2015

Biochemical Society Transactions

View full text Add to dashboard Cite

show abstract

“…An ABC transporter with 20 intrinsic channel activity has yet to be demonstrated in plants but current evidence 21 suggests that AtABCC4 and AtABCC5 play key roles in the regulation of guard cell 22 plasma membrane ion channels [29,32]. 23 …”

Section: Subfamily Cmentioning

confidence: 99%

“…Encoded on the mitochondrial genome; reference [23,24] g. Encoded on the mitochondrial genome [24], part of which is duplicated on Chromosome II in Arabidopsis thaliana [25]. Thus Arabidopsis has one mitochondrial gene and two nuclear genes encoding CcmC homologues.…”

mentioning

confidence: 99%

Plant ABC proteins – a unified nomenclature and updated inventory

Verrier

Bird

Burla

et al. 2008

Trends in Plant Science

649

660

View full text Add to dashboard Cite

The ABC superfamily comprises both membrane-bound transporters and soluble proteins involved in a broad range of processes, many of which are of considerable agricultural, biotechnological and medical potential. Completion of the Arabidopsis and rice genome sequences has revealed a particularly large and diverse complement of plant ABC proteins in comparison with other organisms. Forward and reverse genetics, together with heterologous expression, have uncovered many novel roles for plant ABC proteins, but this progress has been accompanied by a confusing proliferation of names for plant ABC genes and their products. A consolidated nomenclature will provide much-needed clarity and a framework for future research.

show abstract

“…In the latter, 12 proteins are involved in the heme delivery and attachment process, most of them being conserved in plants. In recent years, many of the plant mitochondrial Ccm proteins, either encoded in the mitochondrial or nuclear DNA, have been analyzed and partially characterized (Spielewoy et al, 2001;Meyer et al, 2005;Raczynska et al, 2006;Rayapuram et al, 2007Rayapuram et al, , 2008. In Arabidopsis mitochondria, the CcmC polypeptide is translated from mRNAs that lack the predicted translation stop codon.…”

Section: Function Of Rpf3 In Mitochondria Of Arabidopsis Accession C24mentioning

confidence: 99%

RNA PROCESSING FACTOR3 Is Crucial for the Accumulation of MatureccmCTranscripts in Mitochondria of Arabidopsis Accession Columbia

et al. 2011

View full text Add to dashboard Cite

RNA PROCESSING FACTOR1 (RPF1) and RPF2 are pentatricopeptide repeat (PPR) proteins involved in 5# processing of different mitochondrial mRNAs in Arabidopsis (Arabidopsis thaliana). Both factors are highly similar to RESTORERS OF FERTILITY (RF), which are part of cytoplasmic male sterility/restoration systems in various plant species. These findings suggest a predominant role of RF-like PPR proteins in posttranscriptional 5# processing. To further explore the functions of this group of proteins, we examined a number of T-DNA lines carrying insertions in the corresponding PPR genes. This screening identified a nearly complete absence of mature ccmC transcripts in an At1g62930 T-DNA insertion line, a phenotype that could be restored by the introduction of the intact At1g62930 gene into the mutant. The insertion in this nuclear gene, which we now call RPF3, also leads to a severe reduction of the CcmC protein in mitochondria. The analysis of C24/rpf3-1 F2 hybrids lacking functional RPF3 genes revealed that this gene has less influence on the generation of the mature ccmC 5# transcript end derived from a distinct ccmC 5# upstream configuration found in mitochondrial DNAs from C24 and other accessions. These data show that a particular function of an RF-like protein is required only in connection with a distinct mtDNA configuration. Our new results further substantiate the fundamental role of RF-like PPR proteins in the posttranscriptional generation of plant mitochondrial 5# transcript termini.

show abstract

AtCCMA Interacts with AtCcmB to Form a Novel Mitochondrial ABC Transporter Involved in Cytochrome c Maturation in Arabidopsis

Cited by 50 publications

References 54 publications

Structures and functions of mitochondrial ABC transporters

Structures and functions of mitochondrial ABC transporters

Plant ABC proteins – a unified nomenclature and updated inventory

RNA PROCESSING FACTOR3 Is Crucial for the Accumulation of MatureccmCTranscripts in Mitochondria of Arabidopsis Accession Columbia

Contact Info

Product

Resources

About