At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea ( Ampelopsis grossedentata )

Ma, Rujiang; Zhou, Rongrong; Tong, Runna; Shi, Shuyun; Chen, Xiaoqing

doi:10.1016/j.jchromb.2016.11.037

Cited by 28 publications

(21 citation statements)

References 25 publications

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“…Other interesting chromatographic alternatives for the analysis of flavonoid compounds include HSCCC (Du, Cai, & Xia, 2002;Gao, Ma, & Chen, 2017;Ma, Zhou, & Tong, 2017) and HPCCC (Vieira et al, 2016). HSCCC is an all-liquid chromatographic technique very suitable for preparative isolation of pure compounds (Ignat et al, 2011).…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Liú

Mao

Ding

et al. 2019

Trends in Food Science & Technology

105

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Background: Dihydromyricetin (DMY) is an important plant flavonoid, which has received great attention due to its health-benefiting activities, including antioxidant, antimicrobial, anti-inflammatory, anticancer, antidiabetic and neuroprotective activities. DMY capsules have been sold in US as a nutraceutical supplement to prevent alcoholic hangovers. The major disadvantage associated with DMY is its chemical instability and poor bioavailability caused by the combined effects of its low solubility and poor membrane permeability. This limits its practical use in the food and pharmaceutical fields. Scope and approach: The present paper gives an overview of the current methods for the identification and quantification of DMY. Furthermore, recent findings regarding the main biological properties and chemical stability of DMY, the metabolism of DMY as well as different approaches to increase DMY bioavailability in both aqueous and lipid phases are discussed. Key findings and conclusions: Current trends on identification and quantification of DMY have been focused on spectral and chromatographic techniques. Many factors such as heat, pH, metal ions, could affect the chemical stability of DMY. Despite the diverse biological effects of DMY, DMY faces with the problem of poor bioavailability. Utilization of different delivery systems including solid dispersion, nanocapsule, microemuslion, cyclodextrin inclusion complexes, co-crystallization, phospholipid complexes, and chemical or enzymatic acylation has the potential to improve both the solubility and bioavailability. DMY digested in laboratory animals undergoes reduction, dehydroxylation, methylation, glucuronidation, and sulfation. Novel DMY delivery systems and basic pharmacokinetic studies of encapsulated DMY on higher animals and humans might be required in the future.

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Section: Chromatographic Methodsmentioning

confidence: 99%

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Liú

Mao

Ding

et al. 2019

Trends in Food Science & Technology

105

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“…As shown in Figure 1 and Table 1, [14]. For the tentative identification of iso-dihydromyricetin, the mass data of iso-dihydromyricetin from previous studies [14,25,26] and the current work and those of dihydromyricetin in the current work were comprehensive analyzed. The eight other polyphenolic compounds were all positively identified according to the mass data of their corresponding commercial standards.…”

Section: Characterization Of Main Polyphenols In Vine Tea Extractmentioning

confidence: 99%

Effects of Hot-Water Extract from Vine Tea (Ampelopsis grossedentata) on Acrylamide Formation, Quality and Consumer Acceptability of Bread

Cai

Jia

et al. 2020

Foods

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Acrylamide is a harmful substance that could be inhibited by natural products. Vine tea is an edible herb belonging to the Vitaceae family and has been approved by Chinese authorities as a new food ingredient in 2013. However, the effects of vine tea extract on acrylamide formation and bread quality are rarely investigated. In this study, the polyphenol composition of hot-water extract from vine tea was characterized by ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-ESI-HRMS/MS), and its effects on acrylamide formation, quality, and consumer acceptability of bread were investigated. Vine tea extract and its main polyphenol, dihydromyricetin, significantly inhibited the acrylamide formation in bread, especially the low dose of vine tea extract (1.25 g/kg), which decreased the acrylamide formation by 58.23%. The color and texture of bread were significantly affected by vine tea extract or dihydromyricetin, whereas the moisture content was not changed remarkably. Triangle and paired preference tests indicated that, although the aroma, appearance, and taste of the bread with vine tea extract significantly differ from those of the control bread, vine tea extract did not significantly affect the consumer acceptability. In conclusion, the addition of vine tea extract could be used to develop a new and healthy bread product with low acrylamide content.

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“…The results shown in Table indicated that the S f of methanol/water (2.5:5) and n ‐hexane/ethyl acetate (2.5:5, v/v) was higher than that of other sample solvent systems for their influence on the equilibrium of the solvent system and result in stationary phase loss. It is demonstrated that the first‐dimensional elution solvent system that shared the solvents with the second‐dimensional solvent system was suitable for the interface for the hyphenation . Furthermore, n ‐hexane/ethyl acetate (2.5:5, v/v) and methanol/water (2.5:5, v/v) were investigated for the separation of Sephadex LH‐20 CC, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…To tackle this challenge, a further run in the field of hyphenation of HSCCC with another preparative methods (HSCCC , HPLC , and macroporous resin chromatography ) have then attracted increasing attention. HSCCC combining off‐line with Sephadex LH‐20 column chromatography (Sephadex LH‐20 CC) shows high performance and great flexibility . However, it is labor intensive and time consuming for evaporation and redissolution of the first‐dimensional fractions.…”

Section: Introductionmentioning

confidence: 99%

Online hyphenation of extraction, Sephadex LH‐20 column chromatography, and high‐speed countercurrent chromatography: A highly efficient strategy for the preparative separation of andrographolide from Andrographis paniculata in a single step

Zhang

Wang

Han

et al. 2017

J of Separation Science

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A novel isolation strategy, online hyphenation of ultrasonic extraction, Sephadex LH-20 column chromatography combined with high-speed countercurrent chromatography, was developed for pure compounds extraction and purification. Andrographolide from Andrographis paniculata was achieved only in a single step purification protocol via the present strategy. The crude powder was ultrasonic extracted and extraction was pumped into Sephadex LH-20 column directly to cut the nontarget fractions followed by the second-dimensional high-speed countercurrent chromatography, hyphenated by a six-port valve equipped at the post-end of Sephadex LH-20 column, for the final purification. The results yielded andrographolide with the amount of 1.02 mg and a purity of 98.5% in a single step, indicating that the present method is effective to harvest target compound from medicinal plant.

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At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea ( Ampelopsis grossedentata )

Cited by 28 publications

References 25 publications

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Dihydromyricetin: A review on identification and quantification methods, biological activities, chemical stability, metabolism and approaches to enhance its bioavailability

Effects of Hot-Water Extract from Vine Tea (Ampelopsis grossedentata) on Acrylamide Formation, Quality and Consumer Acceptability of Bread

Online hyphenation of extraction, Sephadex LH‐20 column chromatography, and high‐speed countercurrent chromatography: A highly efficient strategy for the preparative separation of andrographolide from Andrographis paniculata in a single step

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