At-line bioprocess monitoring by immunoassay with rotationally controlled serial siphoning and integrated supercritical angle fluorescence optics

Nwankire, Charles; Donohoe, Gerard G.; Zhang, Xin; Siegrist, Jonathan; Somers, Martin; Kurzbuch, Dirk; Monaghan, Ruairi; Κιτσαρά, Μαρία; Burger, Robert; Hearty, Stephen; Murrell, Julie; Martin, Christopher; Rook, Martha; Barrett, Louise M.; Daniels, Stephen; McDonagh, Colette; O’Kennedy, Richard; Ducrée, Jens

doi:10.1016/j.aca.2013.04.016

Cited by 42 publications

(34 citation statements)

References 33 publications

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“…Through varying the rotationally induced fields relative to the statically defined forces such as interfacial or membrane tension, the force equilibrium at a fluid element can be unbalanced. Such static forces can be implemented by capillary action [17][18][19][20][21], dissolvable films (DFs) [22][23], burstable foils [24], elastomeric membranes [25], dead-end pneumatic chambers [26], siphons [27]-29], and pneumatically enhanced centrifugo-pneumatic siphons (CPSVs) [30][31][32][33].…”

Section: Digital Flow Control Schemesmentioning

confidence: 99%

Lumped-Element Modeling for Rapid Design and Simulation of Digital Centrifugal Microfluidic Systems

Mohammadi¹,

Kinahan²,

Ducrée³

2016

Lab-on-a-Chip Fabrication and Application

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Since the 1990s, centrifugal microfluidic platforms have evolved into a mature technology for the automation of bioanalytical assays in decentralized settings. These "Lab-ona-Disc" (LoaD) systems have already implemented a range of laboratory unit operations (LUOs) such as sample loading, liquid transport, metering, aliquoting, routing, mixing, and washing. By assembling these LUOs in highly functional microfluidic networks, including sample preparation and detection, a sizable portfolio of common test formats such as general chemistry, immunoassays/ protein analysis, nucleic acid testing, and cell counting has been established. The availability of these bioanalytical assay types enables a broad range of applications in fields such as life-science research, biomedical point-ofcare testing and veterinary diagnostics, as well as agrifood, environmental, infrastructural, and industrial monitoring.Recently, a new method of the so-called "event-triggered" flow control has been developed which is independent of the spin rate. These valves, which function in a handshake mode as opposed to the typically batchwise liquid transfers on the "Lab-on-a-Disc" (LoaD) platform, assume a similarly pivotal role as relays and transistors in digital electronics, allowing conditional, logical (flow) control elements. This chapter will describe the modeling of this new generation of "digital" centrifugal microfluidic systems with lowdimensional, lumped-element simulations which have already been instrumental to the modern success story of modern microelectronics.

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Section: Digital Flow Control Schemesmentioning

confidence: 99%

Lumped-Element Modeling for Rapid Design and Simulation of Digital Centrifugal Microfluidic Systems

Mohammadi¹,

Kinahan²,

Ducrée³

2016

Lab-on-a-Chip Fabrication and Application

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“…Siegrist et al 9 developed a more integrated system by implementing a robust serial siphoning method of fluid handling. This system was advanced further by integrating the serial siphoning system with a heterogeneous sandwich immunoassay and surface-confined supercritical angle fluorescence (SAF) detection of human immunoglobulin G (hIgG) 10 . Godino et al 11 integrated a paper siphon into a centrifugal LoaD platform, which introduced rotationally controlled bidirectional flow into the system.…”

Section: Introductionmentioning

confidence: 99%

Phase-selective graphene oxide membranes for advanced microfluidic flow control

et al. 2016

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For the first time, we harness the unique phase-selectivity of chip-integrated graphene oxide (GO) membranes to significantly enhance flow control on centrifugal microfluidic platforms. In this paper, we present novel processes for the assembly of these GO membranes into polymeric microfluidic systems and demonstrate that multilayer GO membranes allow the passage of water while blocking pressurized air and organic solutions.

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“…In recent years there has been increased adoption of rotationally controlled LoaD technologies [1][2] for point-of-care / point-of-use applications in fields such as biomedical diagnostics 3 , bioprocess monitoring 4 and environmental screen-5 ing [5][6] . In this LoaD platform the centrifugal force is the main driver for pumping and liquid handling on a cartridge which typically has similar geometry to conventional optical data storage media such as CDs or DVDs.…”

Section: Introductionmentioning

confidence: 99%

Paper imbibition for timing of multi-step liquid handling protocols on event-triggered centrifugal microfluidic lab-on-a-disc platforms

et al. 2015

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At-line bioprocess monitoring by immunoassay with rotationally controlled serial siphoning and integrated supercritical angle fluorescence optics

Cited by 42 publications

References 33 publications

Lumped-Element Modeling for Rapid Design and Simulation of Digital Centrifugal Microfluidic Systems

Lumped-Element Modeling for Rapid Design and Simulation of Digital Centrifugal Microfluidic Systems

Phase-selective graphene oxide membranes for advanced microfluidic flow control

Paper imbibition for timing of multi-step liquid handling protocols on event-triggered centrifugal microfluidic lab-on-a-disc platforms

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