At a glance: Cellular biology for engineers

Khoshmanesh, Khashayar; Kouzani, A. Z.; Nahavandi, Saeid; Baratchi, Sara; Kanwar, Jagat R.

doi:10.1016/j.compbiolchem.2008.07.010

Cited by 11 publications

(9 citation statements)

References 127 publications

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“…This goal can be achieved in several ways such as decreasing the width of the microchannel, introducing sheath flow to focus the cells along the centreline (Doh and Cho 2005;Moon et al 2009) or implementing circular barriers within the microchannel along the sidewalls upstream of the microelectrodes to reroute the cells off the sidewalls (Khoshmanesh et al 2008). The alternative strategy is to activate the assessing microelectrode array to act in conjunction with the sorting array.…”

Section: Dep Manipulation Of the Dead Yeast Cellsmentioning

confidence: 99%

“…The emergence of micro/nano technologies have enabled us to develop systems, which can efficiently manipulate, separate, monitor and treat biological cells Castillo et al 2009;El-Ali et al 2006;Khoshmanesh et al 2008). Such systems employ mechanical, acoustic, electrical (electrophoresis and dielectrophoresis), magnetic, thermal, optical and chemical phenomena within microfluidic units, as reviewed comprehensively in (Andersson and van den Berg 2003;Castillo et al 2009;Roda et al 2009;Zhang et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of *80% at low medium conductivities and *85% at high medium conductivities.

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Section: Dep Manipulation Of the Dead Yeast Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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“…These cells deform under certain stimulation. They are able to return to the initial shape upon release of the stimulation [27].…”

Section: Particle-based Cell Modellingmentioning

confidence: 99%

Formulation and Simulation of a 3D Mechanical Model of Embryos for Microinjection

Asgari

Asayesh

Lim

et al. 2013

2013 IEEE International Conference on Systems, Man, and Cybernetics

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The understanding of cell manipulation, for example in microinjection, requires an accurate model of the cells. Motivated by this important requirement, a 3D particlebased mechanical model is derived for simulating the deformation of the fish egg membrane and the corresponding cellular forces during microrobotic cell injection. The model is formulated based on the kinematic and dynamic of springdamper configuration with multi-particle joints considering the visco-elastic fluidic properties. It simulates the indentation force feedback as well as cell visual deformation during microinjection. A preliminary simulation study is conducted with different parameter configurations. The results indicate that the proposed particle-based model is able to provide similar deformation profiles as observed from a real microinjection experiment of the zebrafish embryo published in the literature. As a generic modelling approach is adopted, the proposed model also has the potential in applications with different types of manipulation such as micropipette cell aspiration.

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“…We have followed the practice of Khoshmanesh et al [5] in using DI water. The important properties of the medium for the simulations reported herein are density, viscosity, and electric permittivity, which do not vary very much if some small amounts of ions are introduced in the solution to make the system isotonic.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…The advent of micro/nano technologies has enabled us to develop systems capable of e ciently manipulating, separating, monitoring, and treating the biological cells [2][3][4][5]. There are several mechanical, electrical, thermal, optical, acoustic, magnetic, and chemical phenomena within micro uidic systems [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells

et al. 2017

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Abstract. Recently, electrode-based Dielectrophoresis (eDEP) has been used for particle manipulation by means of triangular electrodes. In this theoretical and numerical study, a microchannel with quarter-of-ellipse electrodes is presented and a detailed comparison with triangular electrodes is provided. Electric eld, resultant DEP force, and particle trajectories for each microchannel are evaluated by means of COMSOL Multiphysics 4.4. Afterwards, focusing and separation e ciencies of the systems are assessed and compared. Finally, separation e ciency of our proposed model for live and dead cells is compared with that of our previous model published in the literature [1]. It is demonstrated that our proposed new model has higher lateral DEP force, responsible for cell separation, than the previous triangular-electrode model had. This feature is re ected in the 96% focusing e ciency for 10-micron particles and 100% separation e ciency for live and dead mammalian cells.

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At a glance: Cellular biology for engineers

Cited by 11 publications

References 127 publications

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Formulation and Simulation of a 3D Mechanical Model of Embryos for Microinjection

Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells

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