Astragalus Oral Solution Ameliorates Allergic Asthma in Children by Regulating Relative Contents of CD4+CD25highCD127low Treg Cells

Wang, Wei; Wei, Jing; Liu, Qingbin

doi:10.3389/fped.2018.00255

Cited by 14 publications

(10 citation statements)

References 58 publications

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“…Traditional Chinese medicine is an effective method to treat asthma ( 20 ). A series of studies ( 21 , 22 ) have reported that Astragalus membranaceus (huangqi) has anti-asthma effects, whereby it reduces inflammatory cytokines and improves efficacy by modulating immune balance. Aastragaloside IV, as the main active component of Astragalus membranaceus also exerts strong anti-allergic effects ( 23 ), whereby it protects mice with allergic rhinitis from inflammation ( 24 ), enhances Th1-associated anti-inflammatory cytokines and diminishes Th2-associated pro-inflammatory cytokines ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy

Zhu

Cao

Su³

et al. 2021

Mol Med Rep

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Cycloastragenol (CAG), a secondary metabolite from the roots of Astragalus zahlbruckneri , has been reported to exert anti-inflammatory effects in heart, skin and liver diseases. However, its role in asthma remains unclear. The present study aimed to investigate the effect of CAG on airway inflammation in an ovalbumin (OVA)-induced mouse asthma model. The current study evaluated the lung function and levels of inflammation and autophagy via measurement of airway hyperresponsiveness (AHR), lung histology examination, inflammatory cytokine measurement and western blotting, amongst other techniques. The results demonstrated that CAG attenuated OVA-induced AHR in vivo . In addition, the total number of leukocytes and eosinophils, as well as the secretion of inflammatory cytokines, including interleukin (IL)-5, IL-13 and immunoglobulin E were diminished in bronchoalveolar lavage fluid of the OVA-induced murine asthma model. Histological analysis revealed that CAG suppressed inflammatory cell infiltration and goblet cell secretion. Notably, based on molecular docking simulation, CAG was demonstrated to bind to the active site of autophagy-related gene 4-microtubule-associated proteins light chain 3 complex, which explains the reduced autophagic flux in asthma caused by CAG. The expression levels of proteins associated with autophagy pathways were inhibited following treatment with CAG. Taken together, the results of the present study suggest that CAG exerts an anti-inflammatory effect in asthma, and its role may be associated with the inhibition of autophagy in lung cells.

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Section: Introductionmentioning

confidence: 99%

Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy

Zhu

Cao

Su³

et al. 2021

Mol Med Rep

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“…36 Astragaloside A, a subtype of saponins, could improve children's asthma by regulating CD4 þ CD25 high CD127 low Treg cells. 37 The inhibitory effect of flavonoids and saponins on local inflammation suggests that the effect of AM on AR may be related to the above 2 active ingredients, but further research is necessary to confirm these suspicions. Although polysaccharides have been reported to have an effect in stimulating the secretion of granulocyte-macrophage colony stimulating factor to restore cyclophosphamia-induced immunosuppression, there were still few reports of its application in allergic diseases.…”

Section: Discussionmentioning

confidence: 99%

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Zhong

Jin-Tao

Liu

et al. 2019

Am J Rhinol�Allergy

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Background Astragalus membranaceus (AM), a traditional Chinese medicine, has been used to treat allergic diseases, but the mechanism for treating allergic rhinitis (AR) remains unclear. Objective The purpose of this study was to look at the anti-inflammatory effect of AM on AR and the mechanism of anti-allergy. Methods The mouse model of AR was induced by ovalbumin. Allergic symptoms, number of eosinophils in nasal mucosa, and levels of inflammatory cells in nasal lavage fluid were analyzed. We explored the serum immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, IL-13, interferon-γ (IFN-γ), and IL-10. Besides, the relative mRNA of IL-4, IL-5, and IL-13 was also detected in nasal mucosa tissue. The proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue were analyzed. The mRNA levels of nuclear factor-kappa B p65 (NF-κB p65) and inhibitory kappa B alpha (IκBα), as well as NF-κB p65 DNA binding activity, were tested. We also measured the protein levels of NF-κB p65 and p-NF-κB p65 in nasal mucosa. Results AM could reduce the number of eosinophils in the nasal mucosa and decrease the levels of inflammatory cells in nasal lavage fluid. The serum IgE, IL-4, IL-5, and IL-13 were also decreased, while levels of IFN-γ and IL-10 were increased. The relative mRNA of IL-4, IL-5, and IL-13 was decreased by AM. AM increased the proportion of CD4+ CD25+ Foxp3+ T cells in the spleen and nasal lymphoid tissue. In addition, AM could reduce the activity of NF-kB by inhibiting the mRNA expression and DNA binding activity of NF-κB p65. However, AM had no significant effect on mRNA of IκBα. Above all, AM could reduce the p-NF-κB p65 protein expression of nasal mucosa. Conclusions AM could reduce the secretion of inflammatory cytokines by increasing the level of CD4+ CD25+ Foxp3+ T cells and inhibiting the activation of the NF-κB.

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“…We can nd that treatment effects of WYHY decoction is better than XQL decoction in the regulation of autophagy and improved airway in ammation. The reason may be that WYHY added Ginseng (Radix et Rhizoma) and Astragali (Radix) on the basis of XQL decoction.Many experimental studies have found that Ginseng and Astragali can regulating the autophagy related gene through PI3K-mTOR pathway [44][45][46][47][48][49], and Ginseng and Astragali can also alleviate various kinds of in ammation [50][51][52][53][54]. Therefore, according to the results of this experiment, it can be concluded that WYHY decoction can better regulate autophagy and alleviate airway in ammation of cold asthma rats by added Ginseng (Radix et Rhizoma) and Astragali (Radix).…”

Section: Discussionmentioning

confidence: 81%

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Chang

Zhang

Qing-xiang

et al. 2020

Preprint

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Abstract Background: Airway inflammation plays a pivotal role in cold asthma. Winyanghuayin decoction and Xiaoqinglong decoction can significantly improve airway inflammation. The aim of the present study was to explore the therapeutic effect and mechanism of Winyanghuayin decoction and Xiaoqinglong decoction on OVA-induced cold asthma in rat and compare the effects of Winyanghuayin decoction and Xiaoqinglong decoction for treatment of cold asthma ratsMethods: The cold asthma rat model was induced by OVA and ice water swim stress, treated with Winyanghuayin decoction and Xiaoqinglong decoction respectively. The pulmonary function of asthmatic rats was detected by MIR Spirolab; The pathological changes of lung tissue were observed by HE staining; The autophagy in the lung of rats were observed by transmission electron microscopy; The eosinophils, lymphocytes, macrophages and total cells in bronchoalveolar lavage fluid (BALF) were counted under light microscope. The expression of IL-10\IL-13\TNF-α and TGF-β1 in serum were measured by enzyme-linked immunosorbent assay (ELISA); The protein level of LC3-II\I, ATG, PI3K and mTOR expression in lung tissues were detected by Western blot; Real-time polymerase chain reaction (RT-PCR) detected the expression of autophagy gene ATG3\ATG5\ATG7 and ATG12.Results: Winyanghuayin decoction and Xiaoqinglong decoction can alleviate airway inflammation and improve airway hyper-responsiveness, significantly increased the level of autophagy in lung tissue and decreased the protein level of PI3K and mTOR in lung tissue of cold asthma rats. Winyanghuayin decoction have better regulation ability on autophagy and PI3K-mTOR signaling pathway.Conclusion: Winyanghuayin decoction and Xiaoqinglong decoction may alleviate the airway inflammation symptoms by enhancing autophagy in lung tissue dependent on the PI3K-mTOR signaling pathway. Winyanghuayin decoction was more effective than Xiaoqinglong decoction for treatment of cold asthma.

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Astragalus Oral Solution Ameliorates Allergic Asthma in Children by Regulating Relative Contents of CD4+CD25highCD127low Treg Cells

Cited by 14 publications

References 58 publications

Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy

Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy

Effect of Astragalus membranaceus in Ovalbumin-Induced Allergic Rhinitis Mouse Model

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

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