Astragalus diphtherites FENZL var. diphtherites and Astragalus gymnalopecias RECH. FIL’in gövde ve kök kısımlarından farklı çözücüler ile elde edilen özütlerin invitro antioksidan ve antimikrobiyal özelliklerinin belirlenmesi

Keskin, Cumali; Özen, Hasan Çetin; Toker, Zuhal; Kızıl, Göksel; Kızıl, Murat

doi:10.18016/ksudobil.322478

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…Today about four-fifths of the world's population uses herbal plant medicines (Kale et al, 2019). The identification of bioactive constituents and research on herbal plants have revealed their potential as valuable research material (Keskin et al, 2018). Plant metabolites like tannins, saponins, cardiac glycosides, flavonoids, diterpenoids and triterpenoids possess immunomodulatory function, hence possible role in treatment of diseases with deranged immune function (Renda et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

In-Vitro Cytotoxic and Proliferative Activity of Three Plant Extract on Human Peripheral Blood Mononuclear Cells (PBMCs)

Hamid

Muhammad

Balogun

et al. 2022

Fountain Journal of Natural and Applied Sciences

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Plant products provide a vast source of therapeutics, but not without toxicity. This study evaluated the in vitro cytotoxic and proliferative activity of selected plant extracts on human peripheral blood mononuclear cells (PBMCs). The PBMCs from healthy donors were exposed to varying concentrations (25 µg/ml, 50 µg/ml, 100 µg/ml and 200 µg/ml) of aqueous extracts of young leaves of Mangifera indica (MI) and stem barks of Commiphora kerstingii (CK), and Lannea acida and Acacia sieberiana formulation (LAASF). Trypan blue assay was used to determine the viability of human PBMCs after isolation. Cytotoxicity and proliferation of PBMCs were determined using WST-8 assay. The total viable cell count of the isolated PBMCs in this study was 8000 x 104 cells/ml while viability was 96.15%. The extract of LAASF showed the lowest percentage cytotoxicity (2.63%) at 25 µg/ml concentration, followed by CK (2.70%), then MI (7.71%). There was significant decrease in PBMCs mean absorbance scores across the different concentrations of MI (p=0.008), CK (p<0.0001) and LAASF (p=0.01). There was statistically significant proliferation of PBMCs for MI (p=0.003) and CK (p=0.005) compared to control. However, no significant difference was observed in LAASF on proliferation. Mean absorbance scores significantly decreased with an increase in the concentration of the extracts. The extracts have potential cytotoxicity on the PBMCs at higher concentrations. The extract of MI and CK exhibited higher cytotoxic and proliferative activity on the PBMCs than LAASF. An in-depth study to identify specific immune cells proliferated by the extracts will improve the credence of this study’s findings. Keywords: Herbal medicine, cytotoxicity, cell proliferation, humans, mononuclear leukocytes

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Section: Introductionmentioning

confidence: 99%

In-Vitro Cytotoxic and Proliferative Activity of Three Plant Extract on Human Peripheral Blood Mononuclear Cells (PBMCs)

Hamid

Muhammad

Balogun

et al. 2022

Fountain Journal of Natural and Applied Sciences

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Medicinal Plants and their Traditional Uses

Keskin

2018

JAPB

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It is estimated that there is the number of between 750,000 and 1,000,000 plant species on the world. The 500,000 of them have been identified and named. Around 2000 new flowering plant species are identified and named in each year. The number of plants which have been used for treatment since ancient times shows a steady increase. According to a report released by the World Health Organization (WHO), the number of plants used for treatment is estimated to be around 20,000. The studies on medicinal plants and active substances derived from them have increased the interest in these plants in recent years [1-3]. Plant products are generally preferred and used by patients who have chronic medical conditions including cancers (2%), liver diseases (21%), HIV (22%), asthma (24%) and rheumatologic disorders (26%). Many believe that natural treatment methods are harmless. Whereas, many recent scientific studies have pointed out the serious consequences of the side effects of herbal products such as giving other damages to patients who seek solutions to problems such as obesity. Side

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Sahlep (Dactylorhiza osmanica): Phytochemical Analyses by LC-HRMS, Molecular Docking, Antioxidant Activity, and Enzyme Inhibition Profiles

et al. 2022

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Studies have shown an inverse correlation among age-related illnesses like coronary heart disease and cancer and intake of fruit and vegetable. Given the probable health benefits of natural antioxidants from plants, research on them has increased. Dactylorhiza osmanica is consumed as a food and traditional medicine plant in some regions of Turkey, so evaluation of the biological ability of this species is important. In this study, the amount of phenolic content (LC-HRMS), antioxidant activities and enzyme inhibitory properties of an endemic plant, D. osmanica, were investigated. The antioxidant capacities of an ethanol extract of D. osmanica aerial parts (EDOA) and roots (EDOR) were evaluated with various antioxidant methods. Additionally, the enzyme inhibitory effects of EDOA and EDOR were examined against acetylcholinesterase (AChE), α-glycosidase, and α-amylase enzymes, which are associated with common and global Alzheimer’s disease and diabetes mellitus. The IC50 values of EDOA against the enzymes were found to be 1.809, 1.098, and 0.726 mg/mL, respectively; and the IC50 values of EDOR against the enzymes were found to be 2.466, 0.442, and 0.415 mg/mL, respectively. Additionally, LC-HRMS analyses revealed p-Coumaric acid as the most plentiful phenolic in both EDOA (541.49 mg/g) and EDOR (559.22 mg/g). Furthermore, the molecular docking interaction of p-coumaric acid, quercitrin, and vanillic acid, which are the most plentiful phenolic compounds in the extracts, with AChE, α-glucosidase, and α-amylase, were evaluated using AutoDock Vina software. The rich phenolic content and the effective antioxidant ability and enzyme inhibition potentials of EDOA and EDOR may support the plant’s widespread food and traditional medicinal uses.

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Astragalus diphtherites FENZL var. diphtherites and Astragalus gymnalopecias RECH. FIL’in gövde ve kök kısımlarından farklı çözücüler ile elde edilen özütlerin invitro antioksidan ve antimikrobiyal özelliklerinin belirlenmesi

Cited by 4 publications

References 33 publications

In-Vitro Cytotoxic and Proliferative Activity of Three Plant Extract on Human Peripheral Blood Mononuclear Cells (PBMCs)

In-Vitro Cytotoxic and Proliferative Activity of Three Plant Extract on Human Peripheral Blood Mononuclear Cells (PBMCs)

Medicinal Plants and their Traditional Uses

Sahlep (Dactylorhiza osmanica): Phytochemical Analyses by LC-HRMS, Molecular Docking, Antioxidant Activity, and Enzyme Inhibition Profiles

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