Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae

Saito, Samuel Takashi; Silva, Givaldo; Santos, Regineide Xavier; Gosmann, Grace; Pungartnik, Cristina; Brendel, Martin

doi:10.3390/ijms13032846

Cited by 25 publications

(26 citation statements)

References 29 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Media and solutions were prepared [31,32]. Complete liquid medium (YPD -1.0% yeast extract, 2.0% polypeptone, 2.0% glucose, pH 5.3) was used for routine growth of yeast cells.…”

Section: Strains and Mediamentioning

confidence: 99%

Use of Theobroma cacao Pod Husk-Derived Biofertilizer is Safe as it Poses neither Ecological nor Human Health Risks

Filho¹,

Carvalho-Silva²,

Sousa³

et al. 2017

J Fertil Pestic

Self Cite

View full text Add to dashboard Cite

Cacao farmers use macerated Theobroma cacao pod husks as biofertilizer for restoration of environmentally important soil elements, i.e., N, P, K, Na, Mg, and Ca. The increasing popularity of this organic material for soil management justifies to experimentally ascertaining that its application does not produce environmental or human health risks. We therefore applied a battery of biological tests to assess possible health risks related to the extract of fermented cacao pod husks [CHE]. Minimal inhibitory concentration for selected bacteria and fungi was established and an antiviral assay (equine herpes virus -EHV-1) and insecticide assay (Aedes aegypti larvae), to observe possible environmental impact. Toxicity tests used Artemia salina, hemolytic activity and cytotoxicity were tested using HT-29 e Vero cells, respectively. Genotoxicity and anti-genotoxic activity was tested in the comet assay of leukocytes and a selection of Saccharomyces cerevisiae mutants allowed look for a possible interference of CHE with defined metabolic pathways. None of these established tests used to define toxicity and genotoxicity of chemical compounds indicated that CHE contained substances that would pose such risks. Our safety assessment on bacteria and yeasts, virus, insect larvae, and human leukocytes [3-(4,5-di-methylazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assay] thus indicated that macerated cocoa pod husk can be safely used as biofertilizer.

show abstract

“…Media and solutions were prepared [31,32]. Complete liquid medium (YPD -1.0% yeast extract, 2.0% polypeptone, 2.0% glucose, pH 5.3) was used for routine growth of yeast cells.…”

Section: Strains and Mediamentioning

confidence: 99%

Use of Theobroma cacao Pod Husk-Derived Biofertilizer is Safe as it Poses neither Ecological nor Human Health Risks

Filho¹,

Carvalho-Silva²,

Sousa³

et al. 2017

J Fertil Pestic

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, AECal could have acted as reactive species quenchers. In fact, several studies reported the free radical scavenging ability in Cassia alata extracts [23–26]. AECal - induced synthesis of antioxidant enzymes and inhibition/stimulation of specific proteins involved in DNA damage repair remain to be explored.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects ofCassia alataL.

Ouédraogo

Fabre

et al. 2013

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Aqueous-ethanolic extract of Cassia alata (AECal) and its derived fractions obtained through liquid-liquid fractionation were evaluated for their bronchorelaxant, genotoxic, and antigenotoxic effects. Contractile activity of rats' tracheas in the presence of tested materials, as well as its modifications with different inhibitors and blockers, was isometrically recorded. The antigenotoxic potential of AECal was evaluated on cyclophosphamide- (CP-) induced genotoxicity in the rat. Animals were pretreated with the extract, then liver comet assay was performed. AECal and its chloroformic fractions (CF-AECal) relaxed the contraction induced by Ach, but both were significantly less potent in inhibiting contraction induced by KCl (30 mM; 80 mM). Propranolol, indomethacin, L-NAME, methylene blue, and glibenclamide did not modify the relaxant effect of CF-AECal. TEA altered the response of trachea to CF-AECal. CF-AECal caused a rightward shift without affecting the E max in cumulative concentration-response curves of Ach only at low concentrations. In animals pretreated with the extract, the percentage of CP-induced DNA damage decreased. Our results suggest that (1) muscarinic receptors contribute at least in part to the relaxant effects of CF-AECal; (2) CF-AECal interferes with membrane polarization; and (3) AECal is not genotoxic in vivo and contains chemopreventive phytoconstituents offering protection against CP-induced genotoxicity.

show abstract

“…Preparative HPLC of ethyl acetate phase (fraction B) indicated the presence of kaempferol 3-O-β-glucoside (astragalin) as the major glycoside and isolated it by comparison with reported retention time in literature [13]. Fig.…”

Section: Isolation Of Kaempferol 3-o-β-glucoside (Astragalin)mentioning

confidence: 98%

Isolation of Astragalin From Iraqi Chenopodium Album

Mehdi

Al-Ani

Raoof

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: Chenopodium album L is the species of the genus chenopodium. The Greek name Chenopodium means goosefoot. The plant is native to Asia and Europe. The analysis of the constituents of the Iraqi plant was performed using gas chromatography and high-performance liquid chromatography techniques.Methods: Thin-layer chromatography (TLC) and high-performance TLC chromatographic techniques were used for the detection and isolation of the active constituent found in the plant. Spectral analysis such as nuclear magnetic resonance (NMR) and UV was used to confirm the chemical structure of the compound isolated. Results:Astragalin was isolated and identified by comparison with standard kaempferol 3-O-β-glucoside (astragalin) which was detected as the major glycoside in the polar fraction of the plant. Further, identification of the compound was performed by 1 H-NMR spectroscopy. Conclusion:Astragalin is the major flavonoid glycoside found in the plant.

show abstract

Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae

Cited by 25 publications

References 29 publications

Use of Theobroma cacao Pod Husk-Derived Biofertilizer is Safe as it Poses neither Ecological nor Human Health Risks

Use of Theobroma cacao Pod Husk-Derived Biofertilizer is Safe as it Poses neither Ecological nor Human Health Risks

Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects ofCassia alataL.

Isolation of Astragalin From Iraqi Chenopodium Album

Contact Info

Product

Resources

About