Asthma diagnosis using integrated analysis of eosinophil microRNAs

Rodrigo‐Muñoz, José Manuel; Cañas, J. A.; Sastre, Beatriz; Rego, Natalia; Greif, Gonzalo; Rial, Manuel Jorge; Mínguez, Pablo; Mahíllo‐Fernández, Ignacio; Fernández‐Nieto, Mar; Mora, Inés; Paredes, Pilar; Quirce, Santiago; Sastre, Joaquı́n; Pozo, Victoria del

doi:10.1111/all.13570

Cited by 62 publications

(80 citation statements)

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“…These miRNA could be used to distinguish two clusters of patients with asthma that vary in terms of their eosinophil and serum periostin concentrations. Some of these miRNA were useful for distinguishing patients with asthma from healthy individuals; in particular, miR‐185‐5p was the most useful biomarker candidate . Today, multiple pathological mechanisms are associated with BA pathogenesis.…”

Section: Bronchial Asthma and Mirnamentioning

confidence: 99%

Molecular techniques for respiratory diseases: MicroRNA and extracellular vesicles

et al. 2019

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miRNA are a class of evolutionarily conserved non-coding 19-to 22-nt regulatory RNA. They affect various cellular functions through modulating the transcriptional and post-transcriptional levels of their target mRNA by changing the stability of protein-coding transcripts or attenuating protein translation. miRNA were discovered in the early 1990s, and they have been the focus of new research in both basic and clinical medical sciences. Today, it has become clear that specific miRNA are linked to the pathogenesis of respiratory diseases, as well as cancer and cardiovascular disease. In addition, EV, including exosomes, which are small membrane-bound vesicles secreted by cells, were found to contain various functional miRNA that can be used for diagnostic and therapeutic purposes. As body fluids, such as blood and respiratory secretions, are major miRNA sources in the body, EV carrying extracellular miRNA are considered potentially useful for the diagnosis and assessment of pathological conditions, as well as the treatment of respiratory or other diseases. Although research in the field of lung cancer is actively progressing, studies in other respiratory fields have emerged recently as well. In this review, we provide an update in the topics of miRNA and EV focused on airway inflammatory diseases, such as asthma and COPD, and explore their potential for clinical applications on respiratory diseases.

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Section: Bronchial Asthma and Mirnamentioning

confidence: 99%

Molecular techniques for respiratory diseases: MicroRNA and extracellular vesicles

et al. 2019

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“…We have previously described that differential miRNAs expression obtained from eosinophils of asthmatics compared with healthy individuals was able to cluster asthmatics and healthy subjects. 4 Five of these miRNAs (miR-1246, miR-144-5p, miR-320a, miR-185-5p and miR-21-5p) measured in serum were able to differentiate asthmatics from healthy subjects by statistical models. 4 We hypothesized that some of these miRNAs could be specific to asthma, and they may differentiate asthma from other respiratory diseases.…”

Section: S U P P O R T I N G I N F O R M At I O Nmentioning

confidence: 99%

“…4 Five of these miRNAs (miR-1246, miR-144-5p, miR-320a, miR-185-5p and miR-21-5p) measured in serum were able to differentiate asthmatics from healthy subjects by statistical models. 4 We hypothesized that some of these miRNAs could be specific to asthma, and they may differentiate asthma from other respiratory diseases. Therefore, the aim of the study was to compare these miRNAs between asthmatics, COPD and ACO and to analyse whether they could be used as asthma biomarkers.…”

Section: S U P P O R T I N G I N F O R M At I O Nmentioning

confidence: 99%

“…COPD diagnosis was performed according to the GOLD initiative: postbronchodilator FEV1/FVC ≤ 0.70, with smoking history > 10 packs‐year, in the absence of asthma and ACO by the presence of COPD with ≥200 eosinophils/μL, or a previous asthma diagnosis with smoking history ≥ 20 packs‐year . The asthmatic and healthy controls come from a different cohort previously published, the asthmatics being diagnosed according to the Spanish Asthma Guideline (GEMA) as described in Rodrigo‐Muñoz et al (Table ).…”

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confidence: 99%

“…MiR‐191‐5p was used as endogenous control. Healthy and asthmatic miRNAs expression values are the same as described in Rodrigo‐Muñoz et al (more details on the Data ).…”

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Circulating miRNAs as diagnostic tool for discrimination of respiratory disease: Asthma, asthma‐chronic obstructive pulmonary disease (COPD) overlap and COPD

Rodrigo‐Muñoz

Rial

Sastre

et al. 2019

Allergy

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Circulating miRNAs—A potential tool to identify severe asthma risk?

Kyyaly

Sánchez-Elsner

et al. 2021

Clinical & Translational All

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Background Identifying patients at risk of severe asthma is vitally important given the disproportionate burden of disease imposed by that state. However, biomarkers to support such needs remain elusive. Methods In this letter, we assessed whether specific panels of circulating miRNAs (microRNAs) can differentiate between mild and severe asthma patients as well as between healthy subjects and severe asthma patients. Results To our knowledge, the miRNAs identified in our work such as miR‐28‐3p, miR‐16‐2‐3p, and miR‐210‐3p have not been previously reported as differentially expressed in the serum of severe asthma patients. Conclusion Our findings suggest that miRNA expression profiles may have the capability as potential biomarkers that signal the risk of having severe asthma. As such, these findings have significant novelty and merit wider dissemination to facilitate further work in this field.

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Asthma diagnosis using integrated analysis of eosinophil microRNAs

Abstract: Our data show that miRNAs profile in eosinophils can be used as asthma diagnosis biomarker in serum and that this profile is able to rank asthma severity.

Cited by 62 publications

References 47 publications

Molecular techniques for respiratory diseases: MicroRNA and extracellular vesicles

Molecular techniques for respiratory diseases: MicroRNA and extracellular vesicles

Circulating miRNAs as diagnostic tool for discrimination of respiratory disease: Asthma, asthma‐chronic obstructive pulmonary disease (COPD) overlap and COPD

Circulating miRNAs—A potential tool to identify severe asthma risk?

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