Astaxanthin Protects Steroidogenesis from Hydrogen  Peroxide-Induced Oxidative Stress in Mouse Leydig Cells

Wang, Jyun-Yuan; Lee, Yue-Jia; Chou, Mei‐Chia; Chang, Renin; Chiu, Chien Chao; Liang, Yao-Jen; Wu, Leang‐Shin

doi:10.3390/md13031375

Cited by 39 publications

(32 citation statements)

References 41 publications

(47 reference statements)

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“…In the present study, we determined that the optimal concentration of AST that has the best protective effect on ARPE-19 cells is 40 mg/L, being consistent with a previous study [18]. Here, we only studied its effect on this specific type of cells.…”

Section: Comparative Analysissupporting

confidence: 84%

Astaxanthin protects ARPE-19 cells against oxidative stress injury induced by hydrogen peroxide

Li¹,

Sun

Li³

et al. 2018

Biotechnology & Biotechnological Equipment

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The present study aimed to investigate the protective effect and mechanism of action of astaxanthin (AST) on the oxidative stress injury of adult retinal pigment epithelium-19 (ARPE-19) cells induced by hydrogen peroxide (H 2 O 2). ARPE-19 cells were divided into five groups, including control group, H 2 O 2 model group, AST1 group, AST2 group and AST3 group. MTT was used to determine cell viability. Cell morphology was visualized under an inverted fluorescence microscope. TUNEL assay and flow cytometry was performed to detect cell apoptosis. To examine the levels of ROS, SOD and MDA, we used DCFH-DA, WST-1 and TBA assays. The optimal concentration of AST for increasing cell viability was 40 mg/L. Pretreatment with AST alleviated damages in cell morphology induced by H 2 O 2. Pretreatment with AST had the best protective effect on ARPE-19 cells from oxidative stress injury induced by H 2 O 2. AST treatment had protective effect on ARPE-19 cells against apoptosis. Treatment with AST reduced the apoptotic rate of ARPE-19 cells induced by H 2 O 2. Treatment with AST altered the levels of ROS, SOD and MDA in ARPE-19 cells induced by H 2 O 2. The present study demonstrates that AST protects ARPE-19 cells against oxidative stress injury, probably by inhibiting the production of ROS, elevating the activity of SOD and reducing the content of MDA. Of note, preventive delivery of AST (40 mg/L) has the best effect. The present study also provides a theoretical basis for the prevention and treatment of age-related macular degeneration.

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Section: Comparative Analysissupporting

confidence: 84%

Astaxanthin protects ARPE-19 cells against oxidative stress injury induced by hydrogen peroxide

Li¹,

Sun

Li³

et al. 2018

Biotechnology & Biotechnological Equipment

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“…508 By combining virtual-and structure-based ligand screening approaches, a database of >100 caulerpin analogues was efficiently evaluated in silico for potential inhibitory activity against monoamine oxidase B, 509 while astaxanthin and other algal carotenoids have been the focus of many studies and reviews. [510][511][512][513][514][515][516][517][518]…”

Section: Green Algaementioning

confidence: 99%

Marine natural products

et al. 2017

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Covering: 2015. Previous review: Nat. Prod. Rep., 2016, 33, 382-431This review covers the literature published in 2015 for marine natural products (MNPs), with 1220 citations (792 for the period January to December 2015) referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms. The emphasis is on new compounds (1340 in 429 papers for 2015), together with the relevant biological activities, source organisms and country of origin. Reviews, biosynthetic studies, first syntheses, and syntheses that lead to the revision of structures or stereochemistries, have been included.

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“…Elevated levels of ROS have been shown to be associated with several diseases, including neurodegenerative disease and cancer (3,4). Multiple studies have indicated that ROS inhibits testosterone production in Leydig cells by dissipating mitochondrial membrane potential, and reducing the expression and activity of testicular steroidogenic enzymes (5)(6)(7). Thus, the accumulation of ROS during the ageing process results in reduced levels of testosterone (8).…”

Section: Introductionmentioning

confidence: 99%

Role of peroxiredoxin 2 in H2O2-induced oxidative stress of primary Leydig cells

Duan

Fan

Chen

et al. 2016

Molecular Medicine Reports

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Late‑onset hypogonadism is defined as a condition caused by a decline in the levels of testosterone with aging. One of the major factors contributing to the low levels of testosterone is the accumulation of reactive oxygen species (ROS) in Leydig cells during the ageing process. Peroxiredoxin 2 (Prdx2), a member of the peroxiredoxin family, is an antioxidant protein, the predominant function of which is to neutralize ROS. However, its role in Leydig cells remains to be elucidated. In the present study, primary Leydig cells were exposed to low concentrations of hydrogen peroxide (H2O2) to induce oxidative stress. Cell apoptosis was measured using an Annexin V fluorescein isothiocyanate/propidium iodide apoptosis detection kit and flow cytometry. The level of testosterone was determined by radioimmunoassay, and the mRNA and protein expression levels of Prdx2 were detected by reverse transcription‑polymerase chain reaction and western blotting, respectively. The results revealed a significant increase in cell apoptosis and decrease in testosterone production. In addition, the expression of Prdx2 was decreased by H2O2 in a dose‑ and time‑dependent manner, and this decrease may have been caused by the induction of its molecular structure transformation due to H2O2 elimination. The above findings indicated that Prdx2 may prevent H2O2 accumulation in Leydig cells, and may be important in oxidative stress‑induced apoptosis and decreased testosterone production.

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Astaxanthin Protects Steroidogenesis from Hydrogen Peroxide-Induced Oxidative Stress in Mouse Leydig Cells

Cited by 39 publications

References 41 publications

Astaxanthin protects ARPE-19 cells against oxidative stress injury induced by hydrogen peroxide

Astaxanthin protects ARPE-19 cells against oxidative stress injury induced by hydrogen peroxide

Marine natural products

Role of peroxiredoxin 2 in H2O2-induced oxidative stress of primary Leydig cells

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