Associations of HLA-A, -B and -DRB1 Types with Oral Diseases in Swiss Adults

Abstract

Human leukocyte antigens (HLA) are crucial components of host defense against microbial challenge but the associations of HLA types with oral infectious diseases have not been studied in detail. This prospective cross-sectional study examined associations of HLA-A, -B and -DRB1 types with common oral diseases in a healthy Swiss adult population. 257 subjects (107 m, 150 f, mean age: 43.5 yr; range: 21–58 yr) with known HLA-A, -B and -DRB1 profiles and comprehensive medical records were included. A thorough anamnesis was followed by oral examinations including saliva flow measurements, the DMFT score for cariological status, complete periodontal status with plaque and bleeding indexes as well as assessment of mucosal alterations and temporomandibular dysfunction (TMD). Student’s t-test and Pearson chi-square test were utilized to compare the oral diseases between HLA positive and negative subjects. Bonferroni correction for multiple comparisons was used and PBonf<0.05 was considered statistically significant. HLA types -B15 (PBonf = 0.002), -B51 (PBonf = 0.02) and -DRB1*12 (PBonf = 0.02) were associated with less periodontal disease manifestations. HLA-A32 had a positive association with TMD dysfunction (PBonf = 0.012). No other statistically significant associations were observed. In conclusion, HLA types may contribute to the development of oral diseases in generally healthy Caucasian adults.

HLA Haplotypes and Genotypes Frequencies in Brazilian Chronic Periodontitis Patients

“…HLA-B ∗ 15-DRB1 ∗ 11 was in linkage disequilibrium (Δ′ > 0.80 for all analyzed population). HLA-B ∗ 15 could be associated with the disease: no associations between DRB1 ∗ 11 and CP have been previously reported; however, in agreement with our results, Mauramo et al [ 31 ] found that patients expressing HLA-B ∗ 15 had less clinical periodontal disease manifestation and better periodontal health over a long period of time. HLA-B ∗ 15 positive individuals might have a somewhat peculiar genetic response towards periodontal bacteria challenge contributing to CP development [ 31 ].…”

“…Gender and age ratio was similar between patients and controls. Reichert et al [ 20 ] warned against the fact that gender could represent a confounding variable that should be considered in HLA and periodontitis studies; however, as HLA antigen expression has been reported to not vary as a function of gender [ 29 , 30 ], this expression was probably not taken into consideration in many of the earlier studies on HLA association for periodontal diseases [ 10 , 11 , 13 , 27 , 31 ]. Age was not related to HLA expression but can be related to the disease, although severity and prevalence were mostly related to past disease history, social and behavior factors [ 32 ], and altered inflammatory responses [ 33 ].…”

“…Individuals carrying B ∗ 40 (B60 and B61 antigens) had a three-fold higher risk of developing the disease. Contrarily, in another reporter, HLA-B ∗ 40 was associated with a lower clinical attachment loss in CP; however, this association was lost after Bonferroni correction [ 31 ].…”

The complex pattern of genetic associations of leprosy with HLA class I and class II alleles can be reduced to four amino acid positions

“…associated with protection from periodontal oral infections and recurrent typhoid fever [42,43]. An allele that needs to be considered is HLA-DRB1 � 04:05 which was associated with protection from leprosy in the present as well as other studies [38,44,45].…”

Associations of oral fluid MMP‐8 with periodontitis in Swiss adult subjects

“…Before any clinical assessments, stimulated whole saliva and GCF samples were collected. Paraffin wax stimulated whole saliva (256 samples) was collected as previously described (Mauramo et al., ). GCF samples were collected from mesiobuccal aspects of teeth 16, 12, 24, 36, 32 and 44 (i.e.…”